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AB281567

Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free

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Rabbit Recombinant Monoclonal TLR3 antibody. Carrier free. Suitable for Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CD283, Toll-like receptor 3, TLR3

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Flow Cytometry (Intracellular) - Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (AB281567)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (AB281567)

This data was developed using ab281560, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days cells labelling TLR3 with ab281560 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD11c conjugated to Alexa Fluor® 647 and anti-HLA-DR conjugated to PE, then stained with rabbit IgG or ab281560.Gated on HLA-DR+ population.

Flow Cytometry (Intracellular) - Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (AB281567)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (AB281567)

This data was developed using ab281560, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days cells labelling TLR3 with ab281560 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24454-103

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB288924

Human TLR3 ELISA Kit

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Sandwich ELISA - Human TLR3 ELISA Kit (AB288924)

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Secondary Antibodies

AB150077

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Primary Antibodies

AB242514

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Primary Antibodies

AB253242

Anti-BCMA antibody [EPR22457-260]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-BCMA antibody [EPR22457-260] (AB253242)

  • 0
  • 0
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Reactivity data

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Product details

ab281567 is the carrier-free version of ab281560.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR3 also known as Toll-like receptor 3 is a protein that plays an important role in immune response mechanisms. With a molecular mass of around 104 kDa TLR3 is part of the Toll-like receptor family and expresses mostly in immune cells including dendritic cells and macrophages. It locates mainly on the endosomal membrane where it recognizes double-stranded RNA (dsRNA) which is often a sign of viral infection. TLR3 initiates signaling cascades that lead to the activation of important immune transcription factors.
Biological function summary

TLR3 acts as a sensor for viral and endogenous dsRNA. This receptor forms part of the innate immune system serving as a first line of defense against microbial infections. Upon encountering its ligand TLR3 undergoes conformational changes which allow interaction with the adaptor protein TRIF (TIR-domain-containing adapter-inducing interferon-β). This interaction triggers a downstream signaling cascade that leads to the production of type I interferons and pro-inflammatory cytokines which are essential for mounting an effective antiviral response.

Pathways

TLR3 engages in important signaling cascades like the NF-kB and IRF3 pathways. These pathways are activated by the TRIF-dependent pathway which is unique among the Toll-like receptors. TLR3 influences the expression of genes involved in the inflammatory response and apoptosis. The interactions of TLR3 with other proteins such as MyD88 and IRAK1 are indirect as TLR3 does not depend on the usual MyD88-dependent pathway. This specificity makes TLR3 a critical component in pathogen recognition and response strategies within the immune system.

TLR3 has connections with several conditions primarily viral infections and autoimmune disorders like systemic lupus erythematosus (SLE). Mutations or dysregulation of TLR3 may lead to altered immune responses contributing to the pathogenesis of these diseases. TLR3's association with proteins like TRIF and IRF3 is significant in understanding its role in these conditions. Ongoing research investigates TLR3's potential as a therapeutic target to modulate immune responses in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.
See full target information TLR3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com