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AB317197

Anti-TLR3 antibody [HL2314]

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Rabbit Monoclonal TLR3 antibody. Suitable for IHC-P, WB and reacts with Dog, Mouse, Rat, Human, Cat samples. Immunogen corresponding to Recombinant Fragment Protein within Human TLR3.

View Alternative Names

CD283, Toll-like receptor 3, TLR3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [HL2314] (AB317197)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [HL2314] (AB317197)

ab317197 detects TLR3 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat tissues. TLR3 stained by ab317197 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [HL2314] (AB317197)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [HL2314] (AB317197)

ab317197 detects TLR3 protein at cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded mouse intestine. TLR3 stained by ab317197 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-TLR3 antibody [HL2314] (AB317197)
  • WB

Supplier Data

Western blot - Anti-TLR3 antibody [HL2314] (AB317197)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317197 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-TLR3 antibody [HL2314] (ab317197) at 1/1000 dilution

Lane 1:

CAPAN-2 whole cell extracts at 30 µg

Lane 2:

MCF-10A whole cell extracts at 30 µg

Lane 3:

293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [HL2314] (AB317197)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [HL2314] (AB317197)

ab317197 detects TLR3 protein at cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded dog brain. TLR3 stained by ab317197 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-TLR3 antibody [HL2314] (AB317197)
  • WB

Supplier Data

Western blot - Anti-TLR3 antibody [HL2314] (AB317197)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317197 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-TLR3 antibody [HL2314] (ab317197) at 1/1000 dilution

Lane 1:

MDCK whole cell extracts at 30 µg

Lane 2:

PG-4 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2314

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat, Cat, Dog

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TLR3.

O15455

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR3 also known as Toll-like receptor 3 is a protein that plays an important role in immune response mechanisms. With a molecular mass of around 104 kDa TLR3 is part of the Toll-like receptor family and expresses mostly in immune cells including dendritic cells and macrophages. It locates mainly on the endosomal membrane where it recognizes double-stranded RNA (dsRNA) which is often a sign of viral infection. TLR3 initiates signaling cascades that lead to the activation of important immune transcription factors.
Biological function summary

TLR3 acts as a sensor for viral and endogenous dsRNA. This receptor forms part of the innate immune system serving as a first line of defense against microbial infections. Upon encountering its ligand TLR3 undergoes conformational changes which allow interaction with the adaptor protein TRIF (TIR-domain-containing adapter-inducing interferon-β). This interaction triggers a downstream signaling cascade that leads to the production of type I interferons and pro-inflammatory cytokines which are essential for mounting an effective antiviral response.

Pathways

TLR3 engages in important signaling cascades like the NF-kB and IRF3 pathways. These pathways are activated by the TRIF-dependent pathway which is unique among the Toll-like receptors. TLR3 influences the expression of genes involved in the inflammatory response and apoptosis. The interactions of TLR3 with other proteins such as MyD88 and IRAK1 are indirect as TLR3 does not depend on the usual MyD88-dependent pathway. This specificity makes TLR3 a critical component in pathogen recognition and response strategies within the immune system.

TLR3 has connections with several conditions primarily viral infections and autoimmune disorders like systemic lupus erythematosus (SLE). Mutations or dysregulation of TLR3 may lead to altered immune responses contributing to the pathogenesis of these diseases. TLR3's association with proteins like TRIF and IRF3 is significant in understanding its role in these conditions. Ongoing research investigates TLR3's potential as a therapeutic target to modulate immune responses in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.
See full target information TLR3
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Product promise

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