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AB13876

Anti-TLR5 antibody [19D759.2]

4

(11 Reviews)

|

(20 Publications)

Mouse Monoclonal TLR5 antibody. Suitable for ICC, IP, WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human TLR5 aa 700-800 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

TIL3, TLR5, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

2 Images
Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (AB13876)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (AB13876)

Flow Cytometry (Intracellular) analysis of TLR5 in 106 Ramos cells using (A) 2μg of isotype control and (B) 2μg of ab13876

Western blot - Anti-TLR5 antibody [19D759.2] (AB13876)
  • WB

Unknown

Western blot - Anti-TLR5 antibody [19D759.2] (AB13876)

Western blot analysis of TLR5 in human Ramos cell lysate (A) and mouse Raw cell,lysate (B) using ab13876 at 2μg/ml

All lanes:

Western blot - Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/mL

Lane 1:

Ramos cell lysate

Lane 2:

Raw cell lysate

Predicted band size: 97 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

19D759.2

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC, ICC/IF, IP, Flow Cyt (Intra), IHC-Fr, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human TLR5 aa 700-800 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60602

Specificity

This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval with citrate buffer pH 6.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>(see Miller et al).</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody</p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval with citrate buffer pH 6.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>(see Miller et al).</p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Toll-like receptor 5 (TLR5) also known as CD285 is a protein that functions mechanically as a pattern recognition receptor. It has a mass of about 98 kDa and is expressed mainly in the tissues of the lung intestine and urogenital tract. TLR5 recognizes pathogen-associated molecular patterns specifically identifying and binding to flagellin a protein component of bacterial flagella. This engagement initiates a signaling cascade that results in an immune response.
Biological function summary

This receptor plays a central role in the innate immune system. TLR5 is part of a complex network of receptors that detect microbial infections and activate immune defense. When TLR5 binds to flagellin it triggers the production of inflammatory cytokines and activation of adaptive immune responses. This interaction helps in mobilizing immune cells to the site of infection providing the body with a robust defense mechanism against bacterial pathogens.

Pathways

TLR5 activation is an important component of the NF-kB signaling and MAPK pathways. These pathways lead to the transcription of genes involved in inflammation and immune response. TLR5 interacts with MyD88 an adaptor protein to facilitate the downstream signaling cascade. This involvement links TLR5 to not only NF-kB pathway but also connects it to other Toll-like receptors further integrating the immune signaling network.

TLR5 has been connected to inflammatory bowel disease and chronic obstructive pulmonary disease (COPD). Abnormal TLR5 signaling can lead to excessive inflammation contributing to these conditions. In COPD TLR5 and its associated cytokines can exacerbate lung inflammation while in inflammatory bowel disease dysregulation may trigger improper intestinal immune responses. Research into TLR5's role in these diseases can illuminate potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response (PubMed : 11323673, PubMed : 18490781). Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota (PubMed : 29934223). Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response (PubMed : 11489966, PubMed : 20855887). Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity).
See full target information TLR5
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Animal science journal = Nihon chikusan Gakkaiho 95:e13972 PubMed38923622

2024

TLR5 ligand induces the gene expression of antimicrobial peptides and CXCL8 through IL-1β gene expression in cultured rumen epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Koki Nishihara,Yutaka Suzuki,Satoshi Haga,Sanggun Roh

Nature communications 15:46 PubMed38167804

2024

Mucosal TLR5 activation controls healthspan and longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Sung Lim,Eun Jae Jeon,Hye Sun Go,Hyung-Jin Kim,Kye Young Kim,Thi Quynh Trang Nguyen,Da Young Lee,Kyu Suk Kim,Federico Pietrocola,Seol Hee Hong,Shee Eun Lee,Kyoung-Shim Kim,Tae-Shin Park,Dong-Hee Choi,Yu-Jin Jeong,Jong-Hwan Park,Hyeon Sik Kim,Jung-Joon Min,Yong Sook Kim,Joon Tae Park,Jae-Ho Cho,Gil-Woo Lee,Ji Hyeon Lee,Hyon E Choy,Sang Chul Park,Chul-Ho Lee,Joon Haeng Rhee,Manuel Serrano,Kyung A Cho

Heliyon 9:e20591 PubMed37860535

2023

Fecal microbiota transplantation regulates TFH/TFR cell imbalance via TLR/MyD88 pathway in experimental autoimmune hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Ma,Jianguo Song,Xueping Chen,Duan Dai,Jianping Chen,Liwen Zhang

Gut 72:2112-2122 PubMed37491158

2023

generated butyrate boosts anti-PD-1 efficacy in colorectal cancer by activating cytotoxic CD8 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Kang,Changan Liu,Yanqiang Ding,Yunbi Ni,Fenfen Ji,Harry Cheuk Hay Lau,Lanping Jiang,Joseph Jy Sung,Sunny H Wong,Jun Yu

BMJ open gastroenterology 10: PubMed37433685

2023

Toll-like receptor 5 tunes hepatic and pancreatic stellate cells activation.

Applications

Unspecified application

Species

Unspecified reactive species

Pietro Di Fazio,Sophia Mielke,Isabell T Böhm,Malte Buchholz,Sami Matrood,Detlef Schuppan,Thaddeus Wissniowski

Phytotherapy research : PTR 37:731-742 PubMed36196887

2022

Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Huang,Tiantian Wu,Youbao Zhong,Jiaqi Huang,Zengping Kang,Bugao Zhou,Haimei Zhao,Duanyong Liu

Nutrients 14: PubMed35893896

2022

and Its Metabolite Butyrate Inhibit Colitis and Upregulate TLR5 through the SP3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guangcong Ruan,Minjia Chen,Lu Chen,Fenghua Xu,Zhifeng Xiao,Ailin Yi,Yuting Tian,Yi Ping,Linling Lv,Yi Cheng,Yanling Wei

Cell death & disease 13:183 PubMed35210436

2022

M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huaili Jiang,Lei Zhou,Na Shen,Xianhui Ning,Daquan Wu,Kanglun Jiang,Xinsheng Huang

Acta neuropathologica communications 8:159 PubMed32912327

2020

Activation of Toll-like receptor 5 in microglia modulates their function and triggers neuronal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Masataka Ifuku,Lukas Hinkelmann,Leonard D Kuhrt,Ibrahim E Efe,Victor Kumbol,Alice Buonfiglioli,Christina Krüger,Philipp Jordan,Marcus Fulde,Mami Noda,Helmut Kettenmann,Seija Lehnardt

Tissue barriers 8:1728165 PubMed32079482

2020

Short-term tissue permeability actions of dextran sulfate sodium studied in a colon organ culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth M Danielsen,Alba De Haro Hernando,Mohammad Yassin,Karina Rasmussen,Jørgen Olsen,Gert H Hansen,E Michael Danielsen
View all publications
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