JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB37071

Anti-TLR5 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal TLR5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TLR5.

View Alternative Names

TIL3, TLR5, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TLR5 antibody (AB37071)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TLR5 antibody (AB37071)

ab37071 at 10μg/ml staining TLR5 in THP-1 cells by ICC/IF

Western blot - Anti-TLR5 antibody (AB37071)
  • WB

Unknown

Western blot - Anti-TLR5 antibody (AB37071)

Lane 1:

Western blot - Anti-TLR5 antibody (ab37071) at 0.5 µg/mL

Lane 2:

Western blot - Anti-TLR5 antibody (ab37071) at 1 µg/mL

Lane 3:

Western blot - Anti-TLR5 antibody (ab37071) at 2 µg/mL

All lanes:

THP 1 cell lysate at 15 µg

Predicted band size: 97 kDa

Observed band size: 65 kDa,97 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-TLR5 antibody (AB37071)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TLR5 antibody (AB37071)

Immunofluorescence of TLR5 in THP1 cells using ab37071 at 10 ug/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TLR5. The exact immunogen used to generate this antibody is proprietary information.

O60602

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified by affinity chromatography
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Toll-like receptor 5 (TLR5) also known as CD285 is a protein that functions mechanically as a pattern recognition receptor. It has a mass of about 98 kDa and is expressed mainly in the tissues of the lung intestine and urogenital tract. TLR5 recognizes pathogen-associated molecular patterns specifically identifying and binding to flagellin a protein component of bacterial flagella. This engagement initiates a signaling cascade that results in an immune response.
Biological function summary

This receptor plays a central role in the innate immune system. TLR5 is part of a complex network of receptors that detect microbial infections and activate immune defense. When TLR5 binds to flagellin it triggers the production of inflammatory cytokines and activation of adaptive immune responses. This interaction helps in mobilizing immune cells to the site of infection providing the body with a robust defense mechanism against bacterial pathogens.

Pathways

TLR5 activation is an important component of the NF-kB signaling and MAPK pathways. These pathways lead to the transcription of genes involved in inflammation and immune response. TLR5 interacts with MyD88 an adaptor protein to facilitate the downstream signaling cascade. This involvement links TLR5 to not only NF-kB pathway but also connects it to other Toll-like receptors further integrating the immune signaling network.

TLR5 has been connected to inflammatory bowel disease and chronic obstructive pulmonary disease (COPD). Abnormal TLR5 signaling can lead to excessive inflammation contributing to these conditions. In COPD TLR5 and its associated cytokines can exacerbate lung inflammation while in inflammatory bowel disease dysregulation may trigger improper intestinal immune responses. Research into TLR5's role in these diseases can illuminate potential therapeutic targets.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response (PubMed : 11323673, PubMed : 18490781). Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota (PubMed : 29934223). Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response (PubMed : 11489966, PubMed : 20855887). Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity).
See full target information TLR5
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:104407 PubMed35663028

2022

Neuregulin-1/ErbB4 signaling modulates HRP2-induced damage to brain cortical organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Harbuzariu,Annette Nti,Keri Oxendine Harp,Juan C Cespedes,Adel Driss,Jonathan K Stiles
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB228493

Anti-TLR5 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR5 antibody - N-terminal (AB228493)

  • 0
  • 0
Primary Antibodies

AB13876

Anti-TLR5 antibody [19D759.2]

Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (AB13876)

  • 4
  • 11
Primary Antibodies

AB62460

Anti-TLR5 antibody

Western blot - Anti-TLR5 antibody (AB62460)

  • 4
  • 4
Primary Antibodies

AB168382

Anti-TLR5 antibody [EPR10373]

RabMAb|Recombinant

Western blot - Anti-TLR5 antibody [EPR10373] (AB168382)

  • 0
  • 0
Primary Antibodies

AB115603

Anti-TLR5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR5 antibody (AB115603)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB209216

Anti-TLR2 antibody [EPR20302-119]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR2 antibody [EPR20302-119] (AB209216)

  • 0
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB37068

Anti-TIL/TLR1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TIL/TLR1 antibody (AB37068)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com