Rabbit Polyclonal TLR6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TLR6.
Images
Publications
Filter by
- Diabetes, metabolic syndrome and obesity : targets and therapy 15:3069-30802022Comprehensive Diagnostics of Diabetic Nephropathy by Transcriptome RNA Sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Lei,Yihua Bai,Yang Fan,Yaling Li,Hongying Jiang,Jiaping WangPubMed 36237968
- International journal of molecular sciences 22:2021Enhances the Intestinal Epithelial Tight Junction Barrier and Protects against Intestinal Inflammation by Targeting the Toll-like Receptor-2 Pathway in an NF-κB-Independent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRana Al-Sadi,Viszwapriya Dharmaprakash,Prashant Nighot,Shuhong Guo,Meghali Nighot,Toan Do,Thomas Y MaPubMed 34360835
- Human vaccines & immunotherapeutics 14:1686-16962018Transcriptomal signatures of vaccine adjuvants and accessory immunostimulation of sentinel cells by toll-like receptor 2/6 agonists.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlex C D Salyer et. al.PubMed 29852079
- PloS one 13:e01992112018Plasmatic membrane toll-like receptor expressions in human astrocytomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabele Fattori Moretti et. al.PubMed 29912993
- International journal of oncology 52:139-1542017Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarina Galoian,Silva Abrahamyan,Gor Chailyan,Amir Qureshi,Parthik Patel,Gil Metser,Alexandra Moran,Inesa Sahakyan,Narine Tumasyan,Albert Lee,Tigran Davtyan,Samvel Chailyan,Armen GaloyanPubMed 29138803
- Gut pathogens 9:92017Role of TLR1, TLR2 and TLR6 in the modulation of intestinal inflammation and elimination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Choteau et. al.PubMed 28289440
- Molecular medicine reports 15:1243-12512017Developmental expression of Toll‑like receptors in the guinea pig lung.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingjie Ma et. al.PubMed 28098883
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TLR6. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2C9
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides (PubMed:20037584). In response to diacylated lipopeptides, forms the activation cluster TLR2:TLR6:CD14:CD36, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2 (PubMed:11441107). In complex with TLR4, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion (PubMed:11441107, PubMed:20037584).
Alternative names
CD286, Toll-like receptor 6, TLR6
Recommended products
Rabbit Polyclonal TLR6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TLR6.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TLR6. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2C9
- Purification technique
- Affinity purification Immunogen
- Specificity
TLR6 antibody is specific to TLR6. It does not recognize TLR1.
- Concentration
- Purification notes
This antibody was purified by affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Toll-like receptor 6 (TLR6) acts as an important component of the innate immune system. TLR6 is alternatively known as CD286 and has an approximate molecular mass of 91 kDa. This protein is predominantly expressed in immune cells such as monocytes dendritic cells and macrophages. It localizes mainly on the cell surface and is involved in recognizing pathogen-associated molecular patterns specifically diacyl lipopeptides and peptidoglycans.
- Biological function summary
TLR6 plays a role in pathogen recognition and activation of inflammatory responses. It often forms a functional heterodimer with TLR2 a necessity for its function. Through this complex TLR6 mediates the recognition of bacterial lipoproteins and triggers downstream signaling involved in immune cell activation and cytokine production. This signaling increases the host's immune response to bacterial infections by promoting the release of pro-inflammatory cytokines.
- Pathways
TLR6 initiates signaling cascades that are significant in the immune system such as the NF-kB and MAPK pathways. TLR6 partners with TLR2 to activate the MyD88-dependent signaling pathway an important pathway in innate immunity that affects transcription of pro-inflammatory genes. Interaction with these pathways involves proteins like MyD88 and TRAF6 which are central components in signal transduction leading to inflammation and antimicrobial responses.
- Associated diseases and disorders
TLR6 contributes to the pathogenesis of inflammatory and autoimmune diseases. Research indicates its association with conditions like rheumatoid arthritis and asthma where it influences inflammation levels and immune system dysregulation. TLR6 through its interaction with TLR2 affects the differentiation and activation of immune cells leading to overactive immune responses observed in these disorders. Its role in inflammation makes it a potential target for therapeutic intervention in diseases related to immune system dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-TLR6 antibody (ab37072)
Western Blot of Daudi cell lysate labeling TLR6 with Anti-TLR6 antibody (ab37072) at 1μg/ml in (1) the absence and (2) the presence of blocking peptide..
All lanes: Western blot - Anti-TLR6 antibody (ab37072)
Predicted band size: 92 kDa
Western blot - Anti-TLR6 antibody (ab37072)
Lane 1: Western blot - Anti-TLR6 antibody (ab37072) at 0.5 µg/mL
Lane 2: Western blot - Anti-TLR6 antibody (ab37072) at 1 µg/mL
Lane 3: Western blot - Anti-TLR6 antibody (ab37072) at 2 µg/mL
All lanes: Jurkat cell lysate at 15 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 100 kDa, 55 kDa, 80 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TLR6 antibody (ab37072)
ab37072 at 10 µg/ml staining TLR6 in Jurkat cells by ICC/IF
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com