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AB124928

Anti-TLR7 antibody [EPR2088(2)]

4

(5 Reviews)

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(19 Publications)

Rabbit Recombinant Monoclonal TLR7 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 19 publications.

View Alternative Names

UNQ248/PRO285, TLR7, Toll-like receptor 7

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (AB124928)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (AB124928)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with purified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (AB124928)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (AB124928)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (AB124928)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (AB124928)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-TLR7 antibody [EPR2088(2)] (AB124928)
  • WB

Supplier Data

Western blot - Anti-TLR7 antibody [EPR2088(2)] (AB124928)

Blocking and diluting buffer and concentration : 5% NFDM /TBST. We recommend not boiling the samples after lysis to get desired WB results.

All lanes:

Western blot - Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 1/1000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (boiled) at 15 µg

Lane 2:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (unboiled) at 15 µg

Lane 3:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate (boiled) at 15 µg

Lane 4:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate (unboiled) at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 121 kDa

Observed band size: 140 kDa

false

Exposure time: 20s

OI-RD Scanning - Anti-TLR7 antibody [EPR2088(2)] (AB124928)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TLR7 antibody [EPR2088(2)] (AB124928)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2088(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p><strong>For unpurified use at 1/30.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/300.</strong></p><p><strong>To achieve desired WB results, do not to boil samples after lysis.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR7 also known as Toll-like receptor 7 plays a mechanical role in the immune system by recognizing single-stranded RNA molecules. This receptor with a mass of approximately 120 kDa is a member of the Toll-like receptor family. TLR7 mainly expresses in immune cells like plasmacytoid dendritic cells and B cells. Its location on the endosomal surface allows it to detect viral RNA and trigger downstream signaling cascades.
Biological function summary

TLR7 induces an immune response by activating pathways that lead to the production of cytokines and type I interferons. It does not function alone but interacts within a complex involving other adaptors such as MyD88. This interaction initiates a signaling cascade that is essential for mounting an acute response to viral infections and boosting adaptive immunity.

Pathways

Single-stranded RNA detection through TLR7 activates the MyD88-dependent pathway which is important for innate immune responses. This pathway includes key components such as NF-κB and IRF7 leading to inflammatory cytokine production. TLR7 links with these proteins working together to activate the host defense mechanism against viral infections.

TLR7 plays a significant role in autoimmunity and viral infections. Aberrant activation of TLR7 has connections with systemic lupus erythematosus (SLE) where it may contribute to pathogenesis by promoting autoantibody production. Additionally TLR7 influences the immune response in chronic infections like hepatitis C where it operates with proteins like MyD88 and contributes to disease progression and severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed : 14976261, PubMed : 32433612). Controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs (PubMed : 12738885, PubMed : 27742543, PubMed : 31608988, PubMed : 32706371, PubMed : 35477763). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed : 27742543). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively (PubMed : 27742543, PubMed : 32706371).
See full target information TLR7
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Nature cancer 6:175-193 PubMed39849091

2025

Systemic IFN-I combined with topical TLR7/8 agonists promotes distant tumor suppression by c-Jun-dependent IL-12 expression in dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Sanlorenzo,Philipp Novoszel,Igor Vujic,Tommaso Gastaldi,Martina Hammer,Ourania Fari,Cristiano De Sa Fernandes,Alina D Landau,Bilge V Göcen-Oguz,Martin Holcmann,Babak Monshi,Klemens Rappersberger,Agnes Csiszar,Maria Sibilia

Journal of the European Academy of Dermatology and Venereology : JEADV 38:e816-e819 PubMed38407349

2024

Increased expression of in situ CD123 and reduced Toll-like receptor 7/9 signalling pathway suggest impaired activation of plasmacytoid dendritic cells in recurrent erythema multiforme.

Applications

Unspecified application

Species

Unspecified reactive species

N F Dias de Oliveira,C G Santi,C W Maruta,D Miyamoto,N V Pereira,M N Sotto,L F F da Silva,V Aoki

Cell death discovery 9:315 PubMed37626060

2023

Neutrophil extracellular traps contribute to immunothrombosis formation via the STING pathway in sepsis-associated lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shuainan Zhu,Ying Yu,Mengdi Qu,Zhiyun Qiu,Hao Zhang,Changhong Miao,Kefang Guo

Frontiers in immunology 14:1177968 PubMed37465687

2023

Identification and validation of immune-associated NETosis subtypes and biomarkers in anti-neutrophil cytoplasmic antibody associated glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Tao,Yiqing He,Lijuan Li,Yuyan Li,Wenwen Liao,Haihang Nie,Ping Gao

Frontiers in pharmacology 13:952775 PubMed36210830

2022

Triptolide in the treatment of systemic lupus erythematosus - regulatory effects on miR-146a in B cell TLR7 signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,FengQi Zhang,YiNi Gao,MeiJiao Wang,Yan Gao,HaiChang Li,Jing Sun,ChengPing Wen,ZhiJun Xie

Journal of neuroinflammation 18:209 PubMed34530852

2021

TLR7 is expressed by support cells, but not sensory neurons, in ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Becky J Proskocil,Karol Wai,Katherine M Lebold,Mason A Norgard,Katherine A Michaelis,Ubaldo De La Torre,Madeline Cook,Daniel L Marks,Allison D Fryer,David B Jacoby,Matthew G Drake

mBio 12: PubMed33785626

2021

Multiple Viral microRNAs Regulate Interferon Release and Signaling Early during Infection with Epstein-Barr Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Mickaël Bouvet,Stefanie Voigt,Takanobu Tagawa,Manuel Albanese,Yen-Fu Adam Chen,Yan Chen,Devin N Fachko,Dagmar Pich,Christine Göbel,Rebecca L Skalsky,Wolfgang Hammerschmidt

JCI insight 5: PubMed32699192

2020

TLR7 in B cells promotes renal inflammation and Gd-IgA1 synthesis in IgA nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Nuoyan Zheng,Kaifeng Xie,Hongjian Ye,Yu Dong,Bing Wang,Ning Luo,Jinjin Fan,Jiaqing Tan,Wei Chen,Xueqing Yu

JCI insight 5: PubMed32554924

2020

TLR7 dosage polymorphism shapes interferogenesis and HIV-1 acute viremia in women.

Applications

WB

Species

Unspecified reactive species

Pascal Azar,José Enrique Mejía,Claire Cenac,Arnoo Shaiykova,Ali Youness,Sophie Laffont,Asma Essat,Jacques Izopet,Caroline Passaes,Michaela Müller-Trutwin,Pierre Delobel,Laurence Meyer,Jean-Charles Guéry

Sleep & breathing = Schlaf & Atmung 24:1573-1580 PubMed32076951

2020

TLRs and RAGE are elevated in carotid plaques from patients with moderate-to-severe obstructive sleep apnea syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Wioletta Olejarz,Alicja Głuszko,Agata Cyran,Katarzyna Bednarek-Rajewska,Robert Proczka,David F Smith,Stacey L Ishman,Ewa Migacz,Wojciech Kukwa
View all publications
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