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AB210403

Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TLR7 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

UNQ248/PRO285, TLR7, Toll-like receptor 7

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free (AB210403)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free (AB210403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with purified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124928).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free (AB210403)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free (AB210403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124928).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free (AB210403)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free (AB210403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124928).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free (AB210403)
  • WB

Supplier Data

Western blot - Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free (AB210403)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124928). Blocking and diluting buffer and concentration : 5% NFDM /TBST. We recommend not boiling the samples after lysis to get desired WB results.

All lanes:

Western blot - Anti-TLR7 antibody [EPR2088(2)] (<a href='/en-us/products/primary-antibodies/tlr7-antibody-epr20882-ab124928'>ab124928</a>) at 1/1000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (boiled) at 15 µg

Lane 2:

Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate (unboiled) at 15 µg

Lane 3:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate (boiled) at 15 µg

Lane 4:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate (unboiled) at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 121 kDa

Observed band size: 140 kDa

false

Exposure time: 20s

OI-RD Scanning - Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free (AB210403)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TLR7 antibody [EPR2088(2)] - BSA and Azide free (AB210403)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2088(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p><strong>To achieve desired WB results, do not to boil samples after lysis.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab210403 is the carrier-free version of ab124928.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR7 also known as Toll-like receptor 7 plays a mechanical role in the immune system by recognizing single-stranded RNA molecules. This receptor with a mass of approximately 120 kDa is a member of the Toll-like receptor family. TLR7 mainly expresses in immune cells like plasmacytoid dendritic cells and B cells. Its location on the endosomal surface allows it to detect viral RNA and trigger downstream signaling cascades.
Biological function summary

TLR7 induces an immune response by activating pathways that lead to the production of cytokines and type I interferons. It does not function alone but interacts within a complex involving other adaptors such as MyD88. This interaction initiates a signaling cascade that is essential for mounting an acute response to viral infections and boosting adaptive immunity.

Pathways

Single-stranded RNA detection through TLR7 activates the MyD88-dependent pathway which is important for innate immune responses. This pathway includes key components such as NF-κB and IRF7 leading to inflammatory cytokine production. TLR7 links with these proteins working together to activate the host defense mechanism against viral infections.

TLR7 plays a significant role in autoimmunity and viral infections. Aberrant activation of TLR7 has connections with systemic lupus erythematosus (SLE) where it may contribute to pathogenesis by promoting autoantibody production. Additionally TLR7 influences the immune response in chronic infections like hepatitis C where it operates with proteins like MyD88 and contributes to disease progression and severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed : 14976261, PubMed : 32433612). Controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs (PubMed : 12738885, PubMed : 27742543, PubMed : 31608988, PubMed : 32706371, PubMed : 35477763). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed : 27742543). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively (PubMed : 27742543, PubMed : 32706371). In plasmacytoid dendritic cells, RNASET2 endonuclease cooperates with PLD3 or PLD4 5'->3' exonucleases to process RNA and release 2',3'-cyclic guanosine monophosphate (2',3'-cGMP) and cytidine-rich RNA fragments that occupy TLR7 ligand-binding pockets and trigger a signaling-competent state.
See full target information TLR7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastroenterology and hepatology 37:154-163 PubMed34734434

2021

CD155/TIGIT signaling regulates the effector function of tumor-infiltrating CD8+ T cell by NF-κB pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Li,Jihu Ding,Ying Wang,Xianling Wang,Lin Lv
View all publications
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Product promise

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