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AB85859

Anti-TLR8 antibody [44C143]

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(3 Publications)

Mouse Monoclonal TLR8 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TLR8 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD288, UNQ249/PRO286, TLR8, Toll-like receptor 8

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR8 antibody [44C143] (AB85859)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR8 antibody [44C143] (AB85859)

ab85859 at 5 μg/ml staining TLR8 in formalin fixed and paraffin embedded human spleen tissue

Flow Cytometry (Intracellular) - Anti-TLR8 antibody [44C143] (AB85859)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TLR8 antibody [44C143] (AB85859)

ab85859 at 0.5μg in 1x106 Ramos cells Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents TLR8 antibody.

Western blot - Anti-TLR8 antibody [44C143] (AB85859)
  • WB

Unknown

Western blot - Anti-TLR8 antibody [44C143] (AB85859)

All lanes:

Western blot - Anti-TLR8 antibody [44C143] (ab85859) at 2 µg/mL

All lanes:

293 cell lysate transfected with Human TLR8

Predicted band size: 120 kDa

Observed band size: 120 kDa

false

  • 578 PE

    PE Anti-TLR8 antibody [44C143]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

44C143

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

Synthetic Peptide within Human TLR8 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9NR97

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.5 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.005% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR8 also known as Toll-like receptor 8 is a member of the Toll-like receptor family and has a molecular mass of approximately 120 kDa. This receptor is mainly expressed in immune cells such as monocytes macrophages and dendritic cells. The receptor is located within endosomes and is responsible for recognizing single-stranded RNA (ssRNA) from viruses which leads to an immune response. TLR8 plays a part in detecting pathogens and activating innate immunity.
Biological function summary

TLR8 contributes to the immune system by playing a role in the innate immune response specifically in sensing viral components. TLR8 forms part of a larger receptor complex that includes MyD88 (myeloid differentiation primary response 88) an adaptor protein important for signaling processes within innate immunity. Upon recognizing ssRNA TLR8 initiates signaling cascades to trigger the release of inflammatory cytokines and other mediators that contribute to an antiviral response.

Pathways

TLR8 engages in the innate immune signaling pathway and the NF-kB pathway. These pathways are significant for the activation of immune responses and inflammation. MyD88 bridges TLR8 and downstream signaling events leading to the production and release of pro-inflammatory cytokines. In addition to MyD88 connections to molecules like IRAK4 illustrate how TLR8 signaling maintains a robust immune response to pathogen detection.

TLR8 is linked to autoimmune conditions and viral infections. The receptor's activation by viral components can contribute to chronic inflammation seen in autoimmune diseases such as systemic lupus erythematosus. Additionally aberrant TLR8 signaling is associated with increased susceptibility to certain viral infections where its relationship with MyD88 influences both protective and pathological responses in diseases. Understanding TLR8's function may unveil new therapeutic approaches to manage immune-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed : 25297876, PubMed : 32433612). Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2 (PubMed : 31778653). Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses (PubMed : 33718825). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed : 23520111, PubMed : 25599397, PubMed : 26929371, PubMed : 33718825). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively (PubMed : 16737960, PubMed : 17932028, PubMed : 29155428).
See full target information TLR8
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation (London, England) 13:38 PubMed27980459

2016

Increased adipose tissue expression of TLR8 in obese individuals with or without type-2 diabetes: significance in metabolic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Rasheed Ahmad,Shihab Kochumon,Reeby Thomas,Valerie Atizado,Sardar Sindhu

Journal of hepatology 63:1077-85 PubMed26144659

2015

Expression and functionality of Toll- and RIG-like receptors in HepaRG cells.

Applications

Unspecified application

Species

Unspecified reactive species

Souphalone Luangsay,Malika Ait-Goughoulte,Maud Michelet,Océane Floriot,Marc Bonnin,Marion Gruffaz,Michel Rivoire,Simon Fletcher,Hassan Javanbakht,Julie Lucifora,Fabien Zoulim,David Durantel

The Journal of clinical investigation 125:1081-97 PubMed25642772

2015

Inhibition of IRAK1/4 sensitizes T cell acute lymphoblastic leukemia to chemotherapies.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoyang Li,Kenisha Younger,Ronald Gartenhaus,Ann Mary Joseph,Fang Hu,Maria R Baer,Patrick Brown,Eduardo Davila
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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