Anti-TLR8 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody (AB180610)

Immunofluorescence staining of rat spleen tissue stained for TLR8 with ab180610 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody (AB180610)

Immunofluorescence staining of mouse spleen tissue stained for TLR8 with ab180610 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

• WB

Supplier Data

Western blot - Anti-TLR8 antibody (AB180610)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-TLR8 antibody (ab180610) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat AntiRabbit IgG (H+L)

Predicted band size: 120 kDa

true

• WB

CiteAb

Western blot - Anti-TLR8 antibody (AB180610)

TLR8 western blot using anti-TLR8 antibody ab180610. Publication image and figure legend from Yang, B. Y., Cheng, Y. G., et al., 2019, Molecules, PubMed 31181689.

ab180610 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab180610 please see the product overview.

EPD suppressed the activation of the Toll-like receptor 7–myeloid differentiation primary response gene 88–nuclear factor-κB (TLR7/8–MyD88–NF-κB) signaling pathways. (A) The protein expressions of TLR7, TLR8, TRAF6, MyD88, p-IKKα, IKKα, p-IKBα, IKBα, p-NF-κB, and NF-κB were detected using Western blot assay. (B–H) The quantification of protein levels of TLR7, TLR8, TLAF6, MyD88, p-IKKα, IKKα, p-IKBα, IKBα, p-NF-κB, and NF-κB. Values are presented as the means ± SD of three independent experiments. ** p < 0.01 vs. control group, #p < 0.05, ##p < 0.01 vs. model group.

false