AB24185

Anti-TLR8 antibody

Name: Anti-TLR8 antibody (ab24185) | Abcam
Brand: Abcam Limited
SKU: AB24185

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(8 Publications)

Rabbit Polyclonal TLR8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human TLR8.

View Alternative Names

CD288, UNQ249/PRO286, TLR8, Toll-like receptor 8

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody (AB24185)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody (AB24185)

ab25185 at 2μg/ml staining TLR8 in Daudi cells by ICC/IF

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Western blot - Anti-TLR8 antibody (AB24185)

Lane 1:

Western blot - Anti-TLR8 antibody (ab24185) at 0.5 µg/mL

Lane 2:

Western blot - Anti-TLR8 antibody (ab24185) at 1 µg/mL

Lane 3:

Western blot - Anti-TLR8 antibody (ab24185) at 2 µg/mL

All lanes:

Daudi Cell lysate

Predicted band size: 120 kDa

Observed band size: 110 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TLR8. The exact immunogen used to generate this antibody is proprietary information.

Q9NR97

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified by Ion exchange chromatography.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR8 also known as Toll-like receptor 8 is a member of the Toll-like receptor family and has a molecular mass of approximately 120 kDa. This receptor is mainly expressed in immune cells such as monocytes macrophages and dendritic cells. The receptor is located within endosomes and is responsible for recognizing single-stranded RNA (ssRNA) from viruses which leads to an immune response. TLR8 plays a part in detecting pathogens and activating innate immunity.

Biological function summary

TLR8 contributes to the immune system by playing a role in the innate immune response specifically in sensing viral components. TLR8 forms part of a larger receptor complex that includes MyD88 (myeloid differentiation primary response 88) an adaptor protein important for signaling processes within innate immunity. Upon recognizing ssRNA TLR8 initiates signaling cascades to trigger the release of inflammatory cytokines and other mediators that contribute to an antiviral response.

Pathways

TLR8 engages in the innate immune signaling pathway and the NF-kB pathway. These pathways are significant for the activation of immune responses and inflammation. MyD88 bridges TLR8 and downstream signaling events leading to the production and release of pro-inflammatory cytokines. In addition to MyD88 connections to molecules like IRAK4 illustrate how TLR8 signaling maintains a robust immune response to pathogen detection.

TLR8 is linked to autoimmune conditions and viral infections. The receptor's activation by viral components can contribute to chronic inflammation seen in autoimmune diseases such as systemic lupus erythematosus. Additionally aberrant TLR8 signaling is associated with increased susceptibility to certain viral infections where its relationship with MyD88 influences both protective and pathological responses in diseases. Understanding TLR8's function may unveil new therapeutic approaches to manage immune-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed : 25297876, PubMed : 32433612). Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2 (PubMed : 31778653). Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses (PubMed : 33718825). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed : 23520111, PubMed : 25599397, PubMed : 26929371, PubMed : 33718825). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively (PubMed : 16737960, PubMed : 17932028, PubMed : 29155428).

See full target information TLR8

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of medical virology 89:1852-1860 PubMed28480962

2017

Human papillomavirus type 16 E6 oncoprotein promotes proliferation and invasion of non-small cell lung cancer cells through Toll-like receptor 3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Wang,Zhiqiang Zhang,Huimin Cao,Wenyi Niu,Mingying Li,Xiu'e Xi,Jing Wang

BioMed research international 2016:9872138 PubMed28105439

2016

Functional Roles of Pattern Recognition Receptors That Recognize Virus Nucleic Acids in Human Adipose-Derived Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Yu,Yongtao Xu,Fangchao Wang,Can Yang,Guoyan Liu,Xiangfeng Song

European journal of immunology 44:1503-16 PubMed24500834

2014

Dosage of X-linked Toll-like receptor 8 determines gender differences in the development of systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin R Umiker,Shauna Andersson,Luis Fernandez,Parimal Korgaokar,Amma Larbi,Monika Pilichowska,Craig C Weinkauf,Henry H Wortis,John F Kearney,Thereza Imanishi-Kari

PloS one 8:e58808 PubMed23516559

2013

1,25-Dihydroxyvitamin D3 suppresses TLR8 expression and TLR8-mediated inflammatory responses in monocytes in vitro and experimental autoimmune encephalomyelitis in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Li,David J Baylink,Chandra Deb,Claudia Zannetti,Fatima Rajaallah,Weirong Xing,Michael H Walter,K-H William Lau,Xuezhong Qin

Journal of immunology (Baltimore, Md. : 1950) 189:4657-65 PubMed23002436

2012

The TLR7/8 agonist CL097 primes N-formyl-methionyl-leucyl-phenylalanine-stimulated NADPH oxidase activation in human neutrophils: critical role of p47phox phosphorylation and the proline isomerase Pin1.

Applications

Species

Unspecified reactive species

Karama Makni-Maalej,Tarek Boussetta,Margarita Hurtado-Nedelec,Sahra Amel Belambri,Marie-Anne Gougerot-Pocidalo,Jamel El-Benna

PLoS genetics 7:e1001360 PubMed21483755

2011

The Toll-like receptor gene family is integrated into human DNA damage and p53 networks.

Applications

Species

Human

Daniel Menendez,Maria Shatz,Kathleen Azzam,Stavros Garantziotis,Michael B Fessler,Michael A Resnick

Blood 114:5290-8 PubMed19850743

2009

TLR8-dependent TNF-(alpha) overexpression in Fanconi anemia group C cells.

Applications

Species

Human

Scott M Vanderwerf,Johanna Svahn,Susan Olson,R Keaney Rathbun,Christina Harrington,Jane Yates,Winifred Keeble,David C Anderson,Praveen Anur,Noemi F Pereira,Daniela V Pilonetto,Ricardo Pasquini,Grover C Bagby

Proceedings of the National Academy of Sciences of 106:11725-30 PubMed19564611

2009

Toll-like receptor signaling controls reactivation of KSHV from latency.

Applications

Species

Human

Sean M Gregory,John A West,Patrick J Dillon,Chelsey Hilscher,Dirk P Dittmer,Blossom Damania

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TLR8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody (AB24185)

Western blot - Anti-TLR8 antibody (AB24185)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise