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AB134368

Anti-TLR9 antibody [26C593.2]

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(36 Publications)

Anti-TLR9 antibody [26C593.2] (ab134368) is a mouse monoclonal antibody detecting TLR9 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 20 publications

View Alternative Names

CD289, UNQ5798/PRO19605, TLR9, Toll-like receptor 9

5 Images
Western blot - Anti-TLR9 antibody [26C593.2] (AB134368)
  • WB

Unknown

Western blot - Anti-TLR9 antibody [26C593.2] (AB134368)

All lanes:

Western blot - Anti-TLR9 antibody [26C593.2] (ab134368) at 3 µg/mL

Lane 1:

Human peripheral blood mononuclear cells (PBMCs) at 40 µg

Lane 2:

Human intestine tissue lysate at 40 µg

Lane 3:

Mouse intestine tissue lysate at 40 µg

Lane 4:

Rat intestine tissue lysate at 40 µg

Secondary

All lanes:

Goat anti-mouse HRP conjugate

Predicted band size: 115 kDa

Observed band size: 120 kDa

false

Flow Cytometry (Intracellular) - Anti-TLR9 antibody [26C593.2] (AB134368)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TLR9 antibody [26C593.2] (AB134368)

Flow Cytometric analysis of TLR9 in Ramos (Human Burkitt's lymphoma cell line) cells labelled with ab134368 at 0.1 μg (pink), no antibody (blue) or isotype control antibody (green). Goat anti-mouse IgG FITC secondary was used.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody [26C593.2] (AB134368)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody [26C593.2] (AB134368)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TLR9 with ab134368 at 5 μg/ml.

Staining was enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Flow Cytometry (Intracellular) - Anti-TLR9 antibody [26C593.2] (AB134368)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TLR9 antibody [26C593.2] (AB134368)

Flow Cytometric analysis of TLR9 in human PBMC cells labelled with ab134368 at 0.5 μg (red) or an isotype control antibody (green).

Immunocytochemistry/ Immunofluorescence - Anti-TLR9 antibody [26C593.2] (AB134368)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TLR9 antibody [26C593.2] (AB134368)

Immunohistochemical analysis of TLR9 in frozen sections of A549 (Human lung carcinoma cell line) cells, using ab134368 at a 1/100 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

26C593.2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human TLR9 aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9NR96

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Reactivity data

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Product details

What is this antibody validated in?
Anti-TLR9 antibody [26C593.2] (ab134368) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of TLR9?
Anti-TLR9 [26C593.2] (ab134368) specifically detects a band for TLR9 (UniProt: Q9NR96) at a molecular weight of 115kDa.

Trusted by the scientific community
Anti-TLR9 [26C593.2] (ab134368) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 6 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR9 also known as Toll-like receptor 9 is a protein that detects unmethylated CpG motifs which are common in bacterial and viral DNA. This protein weighs about 116 kDa. It mainly expresses in plasmacytoid dendritic cells B cells and in epithelial cells of various tissues. TLR9 locates in the endoplasmic reticulum and translocates to endolysosomes upon activation which is essential for its detection function.
Biological function summary

TLR9 recognizes and binds to microbial DNA triggering an immune response. It is part of the Toll-like receptor family which plays an important role in the innate immune system. TLR9 engages in the formation of complexes with other molecules such as MyD88 an adaptor protein to propagate downstream signaling. This leads to the activation of transcription factors that stimulate cytokine production promoting inflammation and defense against infections.

Pathways

TLR9 participates significantly in the NF-kB activation pathway and the type I interferon signaling cascade. During these processes TLR9 interacts with proteins like IRAK4 and TRAF6. In the NF-kB pathway TLR9's role in the early immune response is coordinated with other Toll-like receptors to detect pathogen-related patterns and initiate adaptive immunity. Its influence on type I interferon signaling facilitates antiviral defense mechanisms.

TLR9's malfunction or altered expression associates with systemic lupus erythematosus (SLE) and cancer. In SLE overactivation of TLR9 leads to autoantibody production which worsens the disease. This can involve the protein BAFF which supports B cell proliferation. In cancer TLR9 can influence tumor growth and the tumor microenvironment affecting disease progression. Its modulation is being researched for therapeutic interventions in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed : 14716310). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 11564765, PubMed : 17932028). Controls lymphocyte response to Helicobacter infection (By similarity). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (PubMed : 23857366).
See full target information TLR9
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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biochemistry 480:6225-6238 PubMed40859095

2025

NETs accelerate aortic valve calcification by promoting M1 macrophage polarization through the TLR9 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Luyao Liu,Zhenzhen Lan,Xincan Liu,Yun Chen,Zhihao Chen,Li Cheng,Ting Hu

Journal of orthopaedic translation 50:274-294 PubMed39902261

2025

Humanin reduces nucleus pulposus cells ferroptosis to alleviate intervertebral disc degeneration: An in vitro and in vivo study.

Applications

Unspecified application

Species

Unspecified reactive species

Daxue Zhu,Zhaoheng Wang,Yanhu Li,Shijie Chen,Xuewen Kang

Cell death & disease 15:598 PubMed39153998

2024

TLR9 activation in large wound induces tissue repair and hair follicle regeneration via γδT cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinhui Li,Tiantian An,Yang Yang,Zhaoyu Xu,Shuaidong Chen,Zumu Yi,Chen Deng,Feng Zhou,Yi Man,Chen Hu

Infection and immunity 92:e0006324 PubMed38899879

2024

Recognition of by Toll-like receptor 9 is altered during persistence.

Applications

Unspecified application

Species

Unspecified reactive species

Aissata Diallo,Grace Overman,Prakash Sah,George W Liechti

The Journal of biological chemistry 300:105744 PubMed38354781

2024

Activation of innate immune receptor TLR9 by mitochondrial DNA plays essential roles in the chemical long-term depression of hippocampal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Naoya Atarashi,Misaki Morishita,Shinji Matsuda

Cells 13: PubMed38201300

2024

TLR9 Monotherapy in Immune-Competent Mice Suppresses Orthotopic Prostate Tumor Development.

Applications

Unspecified application

Species

Unspecified reactive species

Mark A Miles,Raymond Luong,Eunice E To,Jonathan R Erlich,Stella Liong,Felicia Liong,Jessica M Logan,John O'Leary,Doug A Brooks,Stavros Selemidis

Scientific reports 13:19320 PubMed37935734

2023

Toll-like receptor 9-positive plasmacytoid dendritic cells promote Th17 immune responses in oral lichen planus stimulated by epithelium-derived cathepsin K.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Miyahara,Hu Chen,Masafumi Moriyama,Keita Mochizuki,Naoki Kaneko,A S M Rafiul Haque,Akira Chinju,Kazuki Kai,Mizuki Sakamoto,Noriko Kakizoe-Ishiguro,Masaki Yamauchi,Kenichi Ogata,Tamotsu Kiyoshima,Shintaro Kawano,Seiji Nakamura

Journal of microbiology and biotechnology 33:1213-1227 PubMed37416999

2023

The Gut Microbiota of Pregnant Rats Alleviates Fetal Growth Restriction by Inhibiting the TLR9/MyD88 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Tang,Hanmei Li,Dan Li,Jing Peng,Xian Zhang,Weitao Yang

World journal of emergency medicine 14:209-216 PubMed37152539

2023

Mutual promotion of mitochondrial fission and oxidative stress contributes to mitochondrial-DNA-mediated inflammation and epithelial-mesenchymal transition in paraquat-induced pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Wen-Jing Li,Shi-Qiang Chen,Ze Chen,Chen Zhang,Ran Ying,Hong-Bing Liu,Long-Wang Chen,Ya-Hui Tang,Zhong-Qiu Lu,Guang-Ju Zhao

Frontiers in immunology 14:1094871 PubMed36845129

2023

Dual stimulation by autoantigen and CpG fosters the proliferation of exhausted rheumatoid factor-specific CD21 B cells in hepatitis C virus-cured mixed cryoglobulinemia.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Del Padre,Ramona Marrapodi,Ylenia A Minafò,Eva Piano Mortari,Giovanna Radicchio,Chiara Bocci,Laura Gragnani,Alessandro Camponeschi,Stefania Colantuono,Lucia Stefanini,Stefania Basili,Rita Carsetti,Massimo Fiorilli,Milvia Casato,Marcella Visentini
View all publications
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