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AB37154

Anti-TLR9 antibody

5

(1 Review)

|

(28 Publications)

Rabbit Polyclonal TLR9 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human TLR9.

View Alternative Names

CD289, UNQ5798/PRO19605, TLR9, Toll-like receptor 9

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody (AB37154)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody (AB37154)

ab37154 at 2 µg/ml staining TLR9 in mouse spleen cells by IHC.

Immunocytochemistry/ Immunofluorescence - Anti-TLR9 antibody (AB37154)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TLR9 antibody (AB37154)

Immunofluorescence of TLR9 in Mouse Spleen cells using ab37154 at 10 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody (AB37154)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody (AB37154)

Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse spleen tissue labeling TLR9 with Anti-TLR9 antibody (ab37154) at 5μg/ml.

Western blot - Anti-TLR9 antibody (AB37154)
  • WB

Supplier Data

Western blot - Anti-TLR9 antibody (AB37154)

Lane 1:

Western blot - Anti-TLR9 antibody (ab37154) at 0.5 µg/mL

Lane 2:

Western blot - Anti-TLR9 antibody (ab37154) at 1 µg/mL

All lanes:

Mouse spleen cell lysate

Predicted band size: 115 kDa

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Western blot - Anti-TLR9 antibody (AB37154)
  • WB

CiteAb

Western blot - Anti-TLR9 antibody (AB37154)

TLR9 western blot using anti-TLR9 antibody ab37154. Publication image and figure legend from Hua, F., Wang, J., et al., 2011, J Neuroinflammation, PubMed 21549006.

ab37154 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab37154 please see the product overview.

Expression of TLR2, TLR9, Ccl-2 and Cxcl-10 in traumatically injured brain with or without progesterone treatment. The figures show that TLR2, Ccl-2 and Cxcl-10, but not TLR9, significantly increased in traumatically injured brain compared with sham controls. Administration of PROG inhibited the increase of Ccl-2 and Cxcl-10, but increased the expression of TLR9 in sham and injured brain. Representative images of western blots are shown on the top of A and B. Note : a : compared with sham; b : compared with TBI; d : compared with TBI : p < 0.05. Sham : n = 3; Sham+P : n = 3; TBI : n = 5; TBI+P : n = 5.

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TLR9. The exact immunogen used to generate this antibody is proprietary information.

Q9NR96

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Imunoaffinity purified
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR9 also known as Toll-like receptor 9 is a protein that detects unmethylated CpG motifs which are common in bacterial and viral DNA. This protein weighs about 116 kDa. It mainly expresses in plasmacytoid dendritic cells B cells and in epithelial cells of various tissues. TLR9 locates in the endoplasmic reticulum and translocates to endolysosomes upon activation which is essential for its detection function.
Biological function summary

TLR9 recognizes and binds to microbial DNA triggering an immune response. It is part of the Toll-like receptor family which plays an important role in the innate immune system. TLR9 engages in the formation of complexes with other molecules such as MyD88 an adaptor protein to propagate downstream signaling. This leads to the activation of transcription factors that stimulate cytokine production promoting inflammation and defense against infections.

Pathways

TLR9 participates significantly in the NF-kB activation pathway and the type I interferon signaling cascade. During these processes TLR9 interacts with proteins like IRAK4 and TRAF6. In the NF-kB pathway TLR9's role in the early immune response is coordinated with other Toll-like receptors to detect pathogen-related patterns and initiate adaptive immunity. Its influence on type I interferon signaling facilitates antiviral defense mechanisms.

TLR9's malfunction or altered expression associates with systemic lupus erythematosus (SLE) and cancer. In SLE overactivation of TLR9 leads to autoantibody production which worsens the disease. This can involve the protein BAFF which supports B cell proliferation. In cancer TLR9 can influence tumor growth and the tumor microenvironment affecting disease progression. Its modulation is being researched for therapeutic interventions in these conditions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed : 14716310). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 11564765, PubMed : 17932028). Controls lymphocyte response to Helicobacter infection (By similarity). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (PubMed : 23857366).
See full target information TLR9

Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40867523

2025

Artesunate Ameliorates SLE Atherosclerosis Through PPARγ-Driven Cholesterol Efflux Restoration and Disruption of Lipid Raft-Organized TLR9/MyD88 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Zhang,Xinyu Pan,Yuanfang He,Kairong Sun,Zhiyu Wang,Weiyu Tian,Haonan Qiu,Yiqi Wang,Chengping Wen,Juan Chen

Cancers 16: PubMed39123407

2024

Understanding the Role of Toll-Like Receptors 9 in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Umaima Al-Alem,Alaa Al-Saruri,Hasan Bamahros,Abeer M Mahmoud,Emily Sible,Uzma A Hasan

Scientific reports 14:15789 PubMed38982195

2024

Human red blood cells express the RNA sensor TLR7.

Applications

Unspecified application

Species

Unspecified reactive species

L K Metthew Lam,Emily Oatman,Kaitlyn A Eckart,Nathan J Klingensmith,Emily Flowers,Layal Sayegh,Julia Yuen,Rebecca L Clements,Nuala J Meyer,Kellie A Jurado,Andrew E Vaughan,Stephanie C Eisenbarth,Nilam S Mangalmurti

Journal of microbiology and biotechnology 33:1213-1227 PubMed37416999

2023

The Gut Microbiota of Pregnant Rats Alleviates Fetal Growth Restriction by Inhibiting the TLR9/MyD88 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Tang,Hanmei Li,Dan Li,Jing Peng,Xian Zhang,Weitao Yang

EMBO reports 24:e54006 PubMed36416244

2022

Mitophagy bridges DNA sensing with metabolic adaption to expand lung cancer stem-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Liu,Shan Shan,Zixin Yuan,Fengying Wu,Ming Zheng,Ying Wang,Jun Gui,Wei Xu,Chunhong Wang,Tao Ren,Zhenke Wen

Frontiers in molecular neuroscience 15:1008203 PubMed36277489

2022

Neuronal toll like receptor 9 contributes to complete Freund's adjuvant-induced inflammatory pain in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chen,Hui Chen,Xiao-Chen Li,Wen-Li Mi,Yu-Xia Chu,Yan-Qing Wang,Qi-Liang Mao-Ying

Physiological research 71:259-273 PubMed35275698

2022

Protection of toll-like receptor 9 against lipopolysaccharide-induced inflammation and oxidative stress of pulmonary epithelial cells via MyD88-mediated pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhong Qi,Zhijie He,Jiaxin Chen,Mingyuan Ma,Menghua Deng,Yunhai Zhang,Tianwei Ma

Asian journal of andrology 24:380-385 PubMed34643549

2021

Coexpression of TLR9 and VEGF-C is associated with lymphatic metastasis in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Zi Zeng,Zhan-Sen Huang,Hong-Peng Fang,Jie-Ying Wu,Qun-Xiong Huang,Chu-Bin Zhuang,Jing Zhou,Jin-Ming Di

Cancers 13: PubMed34203465

2021

Hydroxychloroquine (HCQ) Modulates Autophagy and Oxidative DNA Damage Stress in Hepatocellular Carcinoma to Overcome Sorafenib Resistance via TLR9/SOD1/hsa-miR-30a-5p/Beclin-1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yao Chen,Vijesh Kumar Yadav,Yi Cheng Chu,Jiann Ruey Ong,Ting-Yi Huang,Kwai-Fong Lee,Kuen-Haur Lee,Chi-Tai Yeh,Wei-Hwa Lee

Biochemical pharmacology 188:114559 PubMed33872571

2021

Overexpression of retinoid X receptor beta provides protection against oxidized low-density lipoprotein-induced inflammation via regulating PGC1α-dependent mitochondrial homeostasis in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zeng,Chun Yan Wang,Jin Xu,Xiao Le Xu
View all publications

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