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AB62577

Anti-TLR9 antibody

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(1 Publication)

Rabbit Polyclonal TLR9 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TLR9.

View Alternative Names

CD289, UNQ5798/PRO19605, TLR9, Toll-like receptor 9

2 Images
Immunocytochemistry - Anti-TLR9 antibody (AB62577)
  • ICC

Unknown

Immunocytochemistry - Anti-TLR9 antibody (AB62577)

ab62577 at 2μg/ml staining TLR9 in Jurkat cells by Immunocytochemistry.

Western blot - Anti-TLR9 antibody (AB62577)
  • WB

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Western blot - Anti-TLR9 antibody (AB62577)

Lane 1:

Western blot - Anti-TLR9 antibody (ab62577) at 0.5 µg/mL

Lane 2:

Western blot - Anti-TLR9 antibody (ab62577) at 1 µg/mL

Lane 3:

Western blot - Anti-TLR9 antibody (ab62577) at 2 µg/mL

All lanes:

Jurkat cell lysate at 15 µg

Predicted band size: 115 kDa

Observed band size: 95 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human TLR9. The exact immunogen used to generate this antibody is proprietary information.

Q9NR96

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLR9 also known as Toll-like receptor 9 is a protein that detects unmethylated CpG motifs which are common in bacterial and viral DNA. This protein weighs about 116 kDa. It mainly expresses in plasmacytoid dendritic cells B cells and in epithelial cells of various tissues. TLR9 locates in the endoplasmic reticulum and translocates to endolysosomes upon activation which is essential for its detection function.
Biological function summary

TLR9 recognizes and binds to microbial DNA triggering an immune response. It is part of the Toll-like receptor family which plays an important role in the innate immune system. TLR9 engages in the formation of complexes with other molecules such as MyD88 an adaptor protein to propagate downstream signaling. This leads to the activation of transcription factors that stimulate cytokine production promoting inflammation and defense against infections.

Pathways

TLR9 participates significantly in the NF-kB activation pathway and the type I interferon signaling cascade. During these processes TLR9 interacts with proteins like IRAK4 and TRAF6. In the NF-kB pathway TLR9's role in the early immune response is coordinated with other Toll-like receptors to detect pathogen-related patterns and initiate adaptive immunity. Its influence on type I interferon signaling facilitates antiviral defense mechanisms.

TLR9's malfunction or altered expression associates with systemic lupus erythematosus (SLE) and cancer. In SLE overactivation of TLR9 leads to autoantibody production which worsens the disease. This can involve the protein BAFF which supports B cell proliferation. In cancer TLR9 can influence tumor growth and the tumor microenvironment affecting disease progression. Its modulation is being researched for therapeutic interventions in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed : 14716310). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 11564765, PubMed : 17932028). Controls lymphocyte response to Helicobacter infection (By similarity). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (PubMed : 23857366).
See full target information TLR9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of 112:E1659-68 PubMed25775528

2015

Heteromerization of chemokine (C-X-C motif) receptor 4 with α1A/B-adrenergic receptors controls α1-adrenergic receptor function.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Tripathi,P Geoff Vana,Tanmay S Chavan,Lioubov I Brueggemann,Kenneth L Byron,Nadya I Tarasova,Brian F Volkman,Vadim Gaponenko,Matthias Majetschak
View all publications
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Product promise

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