Anti-TLS/FUS antibody

• WB

Lab

Western blot - Anti-TLS/FUS antibody (AB23439)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab23439 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-TLS/FUS antibody (ab23439) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

Lane 2:

Jurkat nuclear extract lysate (<a href='/en-us/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 53 kDa

Observed band size: 75 kDa

true

Exposure time: 8min

• WB

AbReview17567****

Western blot - Anti-TLS/FUS antibody (AB23439)

Blocking Step : 5% Milk; 1% BSA for 16 hours at 4°C

All lanes:

Western blot - Anti-TLS/FUS antibody (ab23439) at 1/1000 dilution

All lanes:

Human 293T cell - whole cell lysate at 30000 Cells

Secondary

All lanes:

An HRP Goat polyclonal to Rabbit IgG at 1/5000 dilution

Predicted band size: 53 kDa

Observed band size: 45 kDa,50 kDa,72 kDa

true

This image is courtesy of an anonymous Abreview

• WB

Lab

Western blot - Anti-TLS/FUS antibody (AB23439)

Gel type : MOPS

Blocking buffer : 2% BSA block

All lanes:

Western blot - Anti-TLS/FUS antibody (ab23439) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukaemia cell line from peripheral blood) Nuclear lysate at 20 µg

Lane 3:

NIH 3T3 (Mouse embryo fibroblast cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 50000 µg

Predicted band size: 53 kDa

Observed band size: 75 kDa

false

Exposure time: 2min