JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272077

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TLS/FUS antibody. Suitable for IHC-P, IP, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human FUS aa 1-50.
4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [BLR023E] - BSA free (AB272077)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [BLR023E] - BSA free (AB272077)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for TLS/FUS using ab243880 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243880).

Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [BLR023E] - BSA free (AB272077)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [BLR023E] - BSA free (AB272077)

Formaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling TLS/FUS using ab243880 at 1/250 dilution in ICC/IF analysis. A DyLight594-conjugated goat anti-rabbit IgG was used as the secondary antibody.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243880).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [BLR023E] - BSA free (AB272077)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [BLR023E] - BSA free (AB272077)

Formalin-fixed, paraffin-embedded human breast tissue stained for TLS/FUS using ab243880 at 1/250 dilution in immunohistochemical analysis. A DyLight594-conjugated goat anti-rabbit antibody was used as the secondary.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243880).

Immunoprecipitation - Anti-TLS/FUS antibody [BLR023E] - BSA free (AB272077)
  • IP

Unknown

Immunoprecipitation - Anti-TLS/FUS antibody [BLR023E] - BSA free (AB272077)

TLS/FUS was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243880 at 20 μl per reaction. Western blot was performed on the immunoprecipitate using ab243880 at 1/1000 dilution.

Lane 1 : ab243880 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243880).

All lanes:

Immunoprecipitation - Anti-TLS/FUS antibody [BLR023E] (<a href='/en-us/products/primary-antibodies/tls-fus-antibody-blr023e-ab243880'>ab243880</a>)

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR023E

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human FUS aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P35637

style
left-column

Alternative Products

Primary Antibodies

AB84078

Anti-TLS/FUS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody (AB84078)

  • 5
  • 2
Primary Antibodies

AB23439

Anti-TLS/FUS antibody

Western blot - Anti-TLS/FUS antibody (AB23439)

  • 5
  • 5
Primary Antibodies

AB124923

Anti-TLS/FUS antibody [EPR5812]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [EPR5812] (AB124923)

  • 5
  • 4
Primary Antibodies

AB154141

Anti-TLS/FUS antibody [CL0190]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [CL0190] (AB154141)

  • 0
  • 1
Primary Antibodies

AB245332

Anti-TLS/FUS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody (AB245332)

  • 0
  • 0
Primary Antibodies

AB232065

Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (AB232065)

  • 0
  • 0

Complementary Products

Sample Prep & Detection Kits

AB269823

Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (AB269823)

  • 5
  • 2
Sample Prep & Detection Kits

AB154876

Gold Conjugation Kit (80nm, 20 OD)

Conjugation - Gold Conjugation Kit (80nm, 20 OD) (AB154876)

  • 0
  • 0
Primary Antibodies

AB52600

Anti-hnRNP K antibody [EP943Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (AB52600)

  • 4
  • 2
Primary Antibodies

AB109535

Anti-TDP43 antibody [EPR5810]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-TDP43 antibody [EPR5810] (AB109535)

  • 5
  • 1
Primary Antibodies

AB133288

Anti-EWSR1/EWS antibody [EPR4647]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-EWSR1/EWS antibody [EPR4647] (AB133288)

  • 0
  • 0
Primary Antibodies

AB220163

Anti-YTHDF2 antibody [EPR20318]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-YTHDF2 antibody [EPR20318] (AB220163)

  • 5
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

ab272077 is the BSA-free version of ab243880.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification notes
Recombinant antibody was purified from cell culture supernatant.
Storage buffer
pH: 8.2 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLS/FUS also known as FUS and the FUS protein is an RNA-binding protein involved in various cellular processes. It has a molecular weight of approximately 53 kDa. Expressed extensively in the nucleus FUS/TLS relocates to the cytoplasm under stress conditions. It plays mechanical roles in transcription regulation RNA splicing and mRNA transport. Scientists sometimes refer to FUS as the 'fused in sarcoma' protein because of its involvement in gene fusion events.
Biological function summary

The FUS protein aids in the maintenance and stabilization of mRNA molecules and participates in the formation of stress granules distinct cytoplasmic aggregates of proteins and RNAs. It forms part of a large protein complex alongside other RNA-binding proteins. FUS stabilizes pre-mRNA structures which affects protein expression patterns essential for cell survival and differentiation. Its ability to bind to RNA and DNA indicates its fundamental role in genomic stability.

Pathways

The functions of FUS help regulate RNA metabolism-related pathways and cellular stress response pathways. FUS interacts with proteins like TAF15 and EWSR1 within these pathways showing a complex network of interactions that contribute to RNA maturation and stress granule dynamic formation. The activity of FUS in these pathways ultimately affects cellular homeostasis gene expression modulation and response to cellular stress.

FUS protein mutations or mislocalizations relate to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). These conditions are characterized by the accumulation of FUS or FUS-related aggregates in the cytoplasm leading to neuronal cell death. FUS is also connected to other RNA-binding proteins like TDP-43 which also mislocalizes and forms aggregates in these disorders highlighting the importance of protein homeostasis and normal RNA metabolism in the prevention of these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response (PubMed : 27731383). Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (PubMed : 21256132). Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing (PubMed : 26124092). Also binds its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay (PubMed : 24204307). Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair (PubMed : 10567410). In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis (By similarity).
See full target information FUS

Alternative Names

TLS, FUS, RNA-binding protein FUS, 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com