Anti-TLS/FUS antibody [CL0190]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [CL0190] (AB154141)

Immunohistochemical analysis of Human kidney tissue labeling TLS/FUS with ab154141 at 1/2500 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [CL0190] (AB154141)

Immunohistochemical analysis of Human prostate tissue labeling TLS/FUS with ab154141 at 1/2500 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [CL0190] (AB154141)

Immunohistochemical analysis of Human pancreas tissue labeling TLS/FUS with ab154141 at 1/2500 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [CL0190] (AB154141)

Immunohistochemical analysis of Human stomach tissue labeling TLS/FUS with ab154141 at 1/2500 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [CL0190] (AB154141)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TLS/FUS with ab154141 showing nuclear (without nucleoli) staining in green. Microtubule are visualized in red.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [CL0190] (AB154141)

ab154141 was shown to react with FUS in wild-type HeLa cells in Immunocytochemistry with loss of signal observed in a FUS knockout cell line. Wild-type and Knockout cells were mixed and pelleted at a 1 : 1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 5%BSA+5%goat serum (30min). The cells were then incubated with ab154141 at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-mouse secondary antibody to (Alexa Fluor^® 555) at 0.5 μg/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (knockout) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and knockout cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880).

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

• WB

Supplier Data

Western blot - Anti-TLS/FUS antibody [CL0190] (AB154141)

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

All lanes:

Western blot - Anti-TLS/FUS antibody [CL0190] (ab154141)

Lane 1:

U-251 cells, transfected with control siRNA

Lane 2:

U-251 cells, transfected with target specific siRNA probe #1

Lane 3:

U-251 cells, transfected with target specific siRNA probe #2

Predicted band size: 53 kDa

false

• WB

Collaborator

Western blot - Anti-TLS/FUS antibody [CL0190] (AB154141)

ab154141 was shown to react with FUS in wild-type HeLa cells in Western blot with loss of signal observed in a FUS knockout cell line. Wild-type HeLa and FUS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab154141 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 1/5000 before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-TLS/FUS antibody [CL0190] (ab154141)

Predicted band size: 53 kDa

false

• WB

Lab

Western blot - Anti-TLS/FUS antibody [CL0190] (AB154141)

Lanes 1- 2 : Merged signal (red and green). Green - ab154141 observed at 80 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.

ab154141 was shown to react with TLS/FUS in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266587 (knockout cell lysate ab257100) was used. Wild-type HEK-293T and FUS knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab154141 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TLS/FUS antibody [CL0190] (ab154141) at 1/500 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

FUS knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human FUS (TLS) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-fus-tls-knockout-hek-293t-cell-line-ab266587'>ab266587</a>)

Predicted band size: 53 kDa

Observed band size: 80 kDa

false

• WB

Lab

Western blot - Anti-TLS/FUS antibody [CL0190] (AB154141)

Lanes 1 - 4 : Merged signal (red and green). Green - ab154141 observed at 80 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab154141 was shown to specifically react with TLS/FUS when TLS/FUS knockout samples were used. Wild-type and TLS/FUS knockout samples were subjected to SDS-PAGE. ab154141 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD)preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-TLS/FUS antibody [CL0190] (ab154141) at 1/500 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

TLS/FUS knockout HAP1 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Predicted band size: 53 kDa

false