Anti-TLS/FUS antibody [P3-A6C4] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [P3-A6C4] - BSA and Azide free (AB324851)

This data was developed using ab323832, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized FUS KO HAP1 (FUS knockout human chronic myelogenous leukemia near-haploid cell) cells labelling TLS/FUS with ab323832 at 1/1000 (1.06 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in parental HAP1 cell line (shown in green) and no staining in FUS KO HAP1 cells. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• WB

Supplier Data

Western blot - Anti-TLS/FUS antibody [P3-A6C4] - BSA and Azide free (AB324851)

This data was developed using ab323832, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35289333).

Lane 1 in this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-TLS/FUS antibody [P3-A6C4] (<a href='/en-us/products/primary-antibodies/tls-fus-antibody-p3-a6c4-ab323832'>ab323832</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 73 kDa,35 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-TLS/FUS antibody [P3-A6C4] - BSA and Azide free (AB324851)

This data was developed using ab323832, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35289333).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TLS/FUS antibody [P3-A6C4] (<a href='/en-us/products/primary-antibodies/tls-fus-antibody-p3-a6c4-ab323832'>ab323832</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

FUS knockout HAP1 whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 73 kDa,35 kDa,36 kDa

false

Exposure time: 180s