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AB184011

Anti-TLX3 antibody

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(2 Publications)

Rabbit Polyclonal TLX3 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TLX3 aa 100-200.

View Alternative Names

HOX11L2, TLX3, T-cell leukemia homeobox protein 3, Homeobox protein Hox-11L2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLX3 antibody (AB184011)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLX3 antibody (AB184011)

Immunohistochemical analysis of paraffin-embedded mouse muscle labeling TLX3 using ab184011 at 1/500 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLX3 antibody (AB184011)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLX3 antibody (AB184011)

Immunohostochemical analysis of paraffin-embedded mouse fore brain labeling TLX3 with ab184011 a 1/500

Western blot - Anti-TLX3 antibody (AB184011)
  • WB

Supplier Data

Western blot - Anti-TLX3 antibody (AB184011)

12% SDS PAGE

All lanes:

Western blot - Anti-TLX3 antibody (ab184011) at 1/1000 dilution

Lane 1:

SK-N-SH whole cell lysate at 30 µg

Lane 2:

IMR32 whole cell lysate at 30 µg

Lane 3:

SK-N-AS whole cell lysate at 30 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TLX3 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

O43711

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TLX3 also known as T-cell leukemia homeobox protein 3 is a transcription factor with a mass of approximately 33 kDa. It belongs to the homeobox gene family and plays a role in regulating gene expression. TLX3 shows expression mostly in the nervous system and immature T-cells particularly during development. The protein acts by binding to specific DNA sequences leading to activation or suppression of target genes essential for the control of developmental processes.
Biological function summary

TLX3 influences the fate of neuronal cell types and is critical in determining neurotransmitter identity. It functions as part of a regulatory complex that guides the differentiation of neurons especially in the dorsal spinal cord. Its activity impacts pathways that promote the production of excitatory glutamatergic neurons over inhibitory GABAergic neurons ensuring proper balance in the central nervous system.

Pathways

TLX3 operates in the gene regulatory networks connected to neuronal differentiation and survival. It interacts importantly with the Notch signaling pathway influencing neurogenesis and cell fate decisions. TLX3 shares regulatory pathways with proteins such as TLX1 and influences the expression of downstream genes that are required for neuronal identity and function. These interactions indicate its role in diverse neuronal processes.

TLX3 is linked to T-cell acute lymphoblastic leukemia (T-ALL) and has implications in certain neurodevelopmental disorders. In T-ALL overexpression of TLX3 disrupts normal T-cell development leading to malignancy. It interacts with proteins like TAL1 contributing to leukemogenesis through modulation of gene networks that control cell proliferation and differentiation. Additionally altered TLX3 expression may correlate with developmental issues in neural patterning showing its importance in both cancer and nervous system diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TLX3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 24:102827 PubMed34381965

2021

Transplantable human motor networks as a neuron-directed strategy for spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary T Olmsted,Cinzia Stigliano,Annalisa Scimemi,Tatiana Wolfe,Jose Cibelli,Philip J Horner,Janet L Paluh

International journal of biological sciences 15:1696-1711 PubMed31360112

2019

TLX3 repressed SNAI1-induced epithelial-mesenchymal transition by directly constraining STAT3 phosphorylation and functionally sensitized 5-FU chemotherapy in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Wang,Changwei Dou,Yufeng Wang,Zhikui Liu,Lewis Roberts,Xin Zheng
View all publications
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