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Rabbit Recombinant Monoclonal TMC1 antibody. Suitable for IP, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse samples. Cited in 3 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-TMC1 antibody [EPR19527] (AB199949), expandable thumbnail
  • Immunoprecipitation - Anti-TMC1 antibody [EPR19527] (AB199949), expandable thumbnail
  • Western blot - Anti-TMC1 antibody [EPR19527] (AB199949), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-Fr
Human
Tested
Tested
Tested
Expected
Mouse
Expected
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Pore-forming subunit of the mechanotransducer (MET) non-selective cation channel complex located at the tips of stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system (By similarity). The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, and aided by several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, and the tip-link PCDH15 (By similarity). MET channel is activated by tension in the tip-link extending from the side wall of one stereocilium to the tip of the adjacent shorter stereocilium, where the channel is located (By similarity). TMC1 MET channel is highly permeable to calcium and likely transports monovalent cations (By similarity). Also involved in vestibular hair cells transduction current (By similarity).

Alternative names

Transmembrane channel-like protein 1, Transmembrane cochlear-expressed protein 1, TMC1

Recommended products

Rabbit Recombinant Monoclonal TMC1 antibody. Suitable for IP, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19527
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TMC1 also known as Transmembrane Channel-Like 1 is a protein that plays a vital role in mechanotransduction in hair cells of the inner ear. It is a relatively large protein with a mass of approximately 99 kDa. TMC1 is expressed mainly in cochlear and vestibular hair cells where it is believed to form or contribute to the formation of ion channels. These ion channels are essential for converting mechanical stimuli into electrical signals. This mechanistic function is critical for auditory and balance performance.

Biological function summary

TMC1 influences cellular responses to mechanical stimuli in the sensory cells. TMC1 functions as part of a complex with other proteins such as TMIE and LHFPL5 contributing to the transduction complex in hair cells. This arrangement enables the translation of physical forces into changes in membrane potential an essential process for hearing. Any disruption in this mechanism can result in profound hearing impairment due to hair cell dysfunction.

Pathways

TMC1 is central to the mechanotransduction pathway particularly in hair cells. This protein supports the conversion of sound-induced vibrations into neural signals an important process in the auditory pathway. TMC1 closely associates with proteins such as TMIE and LHFPL5 indicating a collective role in ensuring accurate signal transduction. Disturbances in this pathway can undermine the auditory system's ability to interpret external sounds correctly.

Associated diseases and disorders

TMC1 has significant associations with hearing loss conditions such as DFNA36 a form of autosomal dominant non-syndromic hearing loss and DFNB7/B11 an autosomal recessive hearing impairment. Mutations in TMC1 disrupt its function resulting in defects in the mechanotransduction process in hair cells. This malfunction is often linked to genetic hearing loss where TMC1 mutations lead to deafness through altered interactions with proteins like TMIE and LHFPL5 highlighting the protein's importance in maintaining proper auditory function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-TMC1 antibody [EPR19527] (ab199949), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TMC1 antibody [EPR19527] (ab199949)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells, transfected with mouse TMC1-GFP or GFP only, labeling TMC1 with ab199949 at 1/500 dilution, followed by Alexa Fluor® 594 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) secondary antibody at 1/1000 dilution (red).

    The results show cytoplasmic staining on mouse TMC1-GFP transfected HEK-293T cells.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) ab150115 (Alexa Fluor® 647 Goat anti-Mouse secondary (white)) at 1/1000 dilution.


    The negative controls are as follows:
    -ve control 1: ab199949 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) ab150115 (Alexa Fluor® 647 Goat anti-Mouse secondary (white)) at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 (Alexa Fluor® 594 Goat anti-Rabbit secondary) at 1/1000 dilution.

    Mouse TMC1 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

  • Immunoprecipitation - Anti-TMC1 antibody [EPR19527] (ab199949), expandable thumbnail

    Immunoprecipitation - Anti-TMC1 antibody [EPR19527] (ab199949)

    TMC1 was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, transfected with pcDNA3.1-TMC1, with ab199949 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199949 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1, 10 μg (Input).

    Lane 2: ab199949 IP in HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199949 in HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    Mouse TMC1 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

    All lanes: Immunoprecipitation - Anti-TMC1 antibody [EPR19527] (ab199949)

    Predicted band size: 88 kDa

  • Western blot - Anti-TMC1 antibody [EPR19527] (ab199949), expandable thumbnail

    Western blot - Anti-TMC1 antibody [EPR19527] (ab199949)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Anti-DDDDK tag antibody used is Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody ab1162.

    Mouse TMC1 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

    WB detection of endogenous TMC1 is not shown due to unavailability of appropriate testing material.

    All lanes: Western blot - Anti-TMC1 antibody [EPR19527] (ab199949) at 1/2000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty DDDDK tag expression vector at 2 µg

    Lane 2: HEK-293T whole cell lysate transfected with DDDDK tagged mouse TMC1 at 2 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa

    Observed band size: 87 kDa

    Exposure time: 1s

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Product protocols

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