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AB199949

Anti-TMC1 antibody [EPR19527]

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(6 Publications)

Rabbit Recombinant Monoclonal TMC1 antibody. Suitable for IP, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse samples. Cited in 6 publications.

View Alternative Names

Transmembrane channel-like protein 1, Transmembrane cochlear-expressed protein 1, TMC1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TMC1 antibody [EPR19527] (AB199949)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMC1 antibody [EPR19527] (AB199949)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with mouse TMC1-GFP or GFP only labeling TMC1 with ab199949 at 1/500 dilution followed by Alexa Fluor® 594 Goat anti-Rabbit (ab150080) secondary antibody at 1/1000 dilution (red).

The results show cytoplasmic staining on mouse TMC1-GFP transfected HEK-293T cells.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150115 (Alexa Fluor® 647 Goat anti-Mouse secondary (white)) at 1/1000 dilution.

The negative controls are as follows :
-ve control 1 : ab199949 at 1/500 dilution followed by ab150115 (Alexa Fluor® 647 Goat anti-Mouse secondary (white)) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150080 (Alexa Fluor® 594 Goat anti-Rabbit secondary) at 1/1000 dilution.

Mouse TMC1 plasmid was kindly provided by our collaborator Dr. Yun Shi Nanjing University MARC.

Immunoprecipitation - Anti-TMC1 antibody [EPR19527] (AB199949)
  • IP

Supplier Data

Immunoprecipitation - Anti-TMC1 antibody [EPR19527] (AB199949)

TMC1 was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, transfected with pcDNA3.1-TMC1, with ab199949 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199949 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1, 10 μg (Input).

Lane 2 : ab199949 IP in HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199949 in HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

Mouse TMC1 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

All lanes:

Immunoprecipitation - Anti-TMC1 antibody [EPR19527] (ab199949)

Predicted band size: 88 kDa

false

Western blot - Anti-TMC1 antibody [EPR19527] (AB199949)
  • WB

Supplier Data

Western blot - Anti-TMC1 antibody [EPR19527] (AB199949)

Blocking/Dilution buffer : 5% NFDM/TBST.

Anti-DDDDK tag antibody used is ab1162.

Mouse TMC1 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

WB detection of endogenous TMC1 is not shown due to unavailability of appropriate testing material.

All lanes:

Western blot - Anti-TMC1 antibody [EPR19527] (ab199949) at 1/2000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty DDDDK tag expression vector at 2 µg

Lane 2:

HEK-293T whole cell lysate transfected with DDDDK tagged mouse TMC1 at 2 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 88 kDa

Observed band size: 87 kDa

false

Exposure time: 1s

  • Carrier free

    Anti-TMC1 antibody [EPR19527] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19527

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, IHC-Fr, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMC1 also known as Transmembrane Channel-Like 1 is a protein that plays a vital role in mechanotransduction in hair cells of the inner ear. It is a relatively large protein with a mass of approximately 99 kDa. TMC1 is expressed mainly in cochlear and vestibular hair cells where it is believed to form or contribute to the formation of ion channels. These ion channels are essential for converting mechanical stimuli into electrical signals. This mechanistic function is critical for auditory and balance performance.
Biological function summary

TMC1 influences cellular responses to mechanical stimuli in the sensory cells. TMC1 functions as part of a complex with other proteins such as TMIE and LHFPL5 contributing to the transduction complex in hair cells. This arrangement enables the translation of physical forces into changes in membrane potential an essential process for hearing. Any disruption in this mechanism can result in profound hearing impairment due to hair cell dysfunction.

Pathways

TMC1 is central to the mechanotransduction pathway particularly in hair cells. This protein supports the conversion of sound-induced vibrations into neural signals an important process in the auditory pathway. TMC1 closely associates with proteins such as TMIE and LHFPL5 indicating a collective role in ensuring accurate signal transduction. Disturbances in this pathway can undermine the auditory system's ability to interpret external sounds correctly.

TMC1 has significant associations with hearing loss conditions such as DFNA36 a form of autosomal dominant non-syndromic hearing loss and DFNB7/B11 an autosomal recessive hearing impairment. Mutations in TMC1 disrupt its function resulting in defects in the mechanotransduction process in hair cells. This malfunction is often linked to genetic hearing loss where TMC1 mutations lead to deafness through altered interactions with proteins like TMIE and LHFPL5 highlighting the protein's importance in maintaining proper auditory function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of the mechanotransducer (MET) non-selective cation channel complex located at the tips of stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system (By similarity). The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, and aided by several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, and the tip-link PCDH15 (By similarity). MET channel is activated by tension in the tip-link extending from the side wall of one stereocilium to the tip of the adjacent shorter stereocilium, where the channel is located (By similarity). TMC1 MET channel is highly permeable to calcium and likely transports monovalent cations (By similarity). Also involved in vestibular hair cells transduction current (By similarity).
See full target information TMC1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell research 35:243-264 PubMed39966628

2025

A force-sensitive adhesion GPCR is required for equilibrioception.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Yang,Shu-Hua Zhou,Qi-Yue Zhang,Zhi-Chen Song,Wen-Wen Liu,Yu Sun,Ming-Wei Wang,Xiao-Long Fu,Kong-Kai Zhu,Ying Guan,Jie-Yu Qi,Xiao-Hui Wang,Yu-Nan Sun,Yan Lu,Yu-Qi Ping,Yue-Tong Xi,Zhen-Xiao Teng,Lei Xu,Peng Xiao,Zhi-Gang Xu,Wei Xiong,Wei Qin,Wei Yang,Fan Yi,Ren-Jie Chai,Xiao Yu,Jin-Peng Sun

PeerJ 12:e18543 PubMed39588003

2024

Identification and validation of three diagnostic autophagy-related genes associated with advanced plaques and immune cell infiltration in carotid atherosclerosis based on integrated bioinformatics analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Tiegen Huang,Chen Su,Quanli Su,Yali Nie,Zhenni Xiao,Yao Tang,Jiahao Wang,Xiaotian Luo,Yixin Tang

Stem cell research & therapy 15:4 PubMed38167128

2024

Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Luo,Kaiwen Wu,Xiaolong Zhang,Hongyang Wang,Qiuju Wang

PloS one 18:e0287249 PubMed37352201

2023

The cytosolic N-terminal region of heterologously-expressed transmembrane channel-like protein 1 (TMC1) can be cleaved in HEK293 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Soichiro Yamaguchi,Maho Kamino,Maho Hamamura,Ken-Ichi Otsuguro

Science advances 8:eadc9236 PubMed36542715

2022

GABA signaling triggered by TMC-1/Tmc delays neuronal aging by inhibiting the PKC pathway in .

Applications

Unspecified application

Species

Unspecified reactive species

Jieyu Wu,Liuyang Wang,John F Ervin,Shih-Hsiu J Wang,Erik Soderblom,Dennis Ko,Dong Yan

International journal of molecular sciences 21: PubMed32899784

2020

A Mechanosensitive Channel, Mouse Transmembrane Channel-Like Protein 1 (Mtmc1) Is Translated from a Splice Variant but Not from the Other Variant .

Applications

Unspecified application

Species

Unspecified reactive species

Soichiro Yamaguchi,Maho Hamamura,Ken-Ichi Otsuguro
View all publications
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