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Rabbit Recombinant Monoclonal TMC1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-TMC1 antibody [EPR19527] - BSA and Azide free (AB271966), expandable thumbnail
  • Immunoprecipitation - Anti-TMC1 antibody [EPR19527] - BSA and Azide free (AB271966), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-Fr
Human
Tested
Expected
Tested
Expected
Mouse
Expected
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Pore-forming subunit of the mechanotransducer (MET) non-selective cation channel complex located at the tips of stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system (By similarity). The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, and aided by several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, and the tip-link PCDH15 (By similarity). MET channel is activated by tension in the tip-link extending from the side wall of one stereocilium to the tip of the adjacent shorter stereocilium, where the channel is located (By similarity). TMC1 MET channel is highly permeable to calcium and likely transports monovalent cations (By similarity). Also involved in vestibular hair cells transduction current (By similarity).

Alternative names

Transmembrane channel-like protein 1, Transmembrane cochlear-expressed protein 1, TMC1

Recommended products

Rabbit Recombinant Monoclonal TMC1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR19527
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab271966 is the carrier-free version of Anti-TMC1 antibody [EPR19527] ab199949.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TMC1 also known as Transmembrane Channel-Like 1 is a protein that plays a vital role in mechanotransduction in hair cells of the inner ear. It is a relatively large protein with a mass of approximately 99 kDa. TMC1 is expressed mainly in cochlear and vestibular hair cells where it is believed to form or contribute to the formation of ion channels. These ion channels are essential for converting mechanical stimuli into electrical signals. This mechanistic function is critical for auditory and balance performance.

Biological function summary

TMC1 influences cellular responses to mechanical stimuli in the sensory cells. TMC1 functions as part of a complex with other proteins such as TMIE and LHFPL5 contributing to the transduction complex in hair cells. This arrangement enables the translation of physical forces into changes in membrane potential an essential process for hearing. Any disruption in this mechanism can result in profound hearing impairment due to hair cell dysfunction.

Pathways

TMC1 is central to the mechanotransduction pathway particularly in hair cells. This protein supports the conversion of sound-induced vibrations into neural signals an important process in the auditory pathway. TMC1 closely associates with proteins such as TMIE and LHFPL5 indicating a collective role in ensuring accurate signal transduction. Disturbances in this pathway can undermine the auditory system's ability to interpret external sounds correctly.

Associated diseases and disorders

TMC1 has significant associations with hearing loss conditions such as DFNA36 a form of autosomal dominant non-syndromic hearing loss and DFNB7/B11 an autosomal recessive hearing impairment. Mutations in TMC1 disrupt its function resulting in defects in the mechanotransduction process in hair cells. This malfunction is often linked to genetic hearing loss where TMC1 mutations lead to deafness through altered interactions with proteins like TMIE and LHFPL5 highlighting the protein's importance in maintaining proper auditory function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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