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AB223541

Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TMC1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

Transmembrane channel-like protein 1, Transmembrane cochlear-expressed protein 1, TMC1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (AB223541)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (AB223541)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with mouse TMC1-GFP or GFP only labeling TMC1 with ab199949 at 1/500 dilution followed by Alexa Fluor® 594 Goat anti-Rabbit (ab150080) secondary antibody at 1/1000 dilution (red).

The results show cytoplasmic staining on mouse TMC1-GFP transfected HEK-293T cells.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150115 (Alexa Fluor® 647 Goat anti-Mouse secondary (white)) at 1/1000 dilution.

The negative controls are as follows :
-ve control 1 : ab199949 at 1/500 dilution followed by ab150115 (Alexa Fluor® 647 Goat anti-Mouse secondary (white)) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150080 (Alexa Fluor® 594 Goat anti-Rabbit secondary) at 1/1000 dilution.

Mouse TMC1 plasmid was kindly provided by our collaborator Dr. Yun Shi Nanjing University MARC.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab199949).

Immunoprecipitation - Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (AB223541)
  • IP

Supplier Data

Immunoprecipitation - Anti-TMC1 antibody [EPR19527] - Low endotoxin, Azide free (AB223541)

TMC1 was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, transfected with pcDNA3.1-TMC1, with ab199949 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199949 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1, 10 μg (Input).

Lane 2 : ab199949 IP in HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199949 in HEK-293T whole cell lysate transfected with pcDNA3.1-TMC1.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

Mouse TMC1 plasmid was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199949).

All lanes:

Immunoprecipitation - Anti-TMC1 antibody [EPR19527] (<a href='/en-us/products/primary-antibodies/tmc1-antibody-epr19527-ab199949'>ab199949</a>)

Predicted band size: 88 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19527

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-Fr, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

ab223541 is the carrier-free version of ab199949.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMC1 also known as Transmembrane Channel-Like 1 is a protein that plays a vital role in mechanotransduction in hair cells of the inner ear. It is a relatively large protein with a mass of approximately 99 kDa. TMC1 is expressed mainly in cochlear and vestibular hair cells where it is believed to form or contribute to the formation of ion channels. These ion channels are essential for converting mechanical stimuli into electrical signals. This mechanistic function is critical for auditory and balance performance.
Biological function summary

TMC1 influences cellular responses to mechanical stimuli in the sensory cells. TMC1 functions as part of a complex with other proteins such as TMIE and LHFPL5 contributing to the transduction complex in hair cells. This arrangement enables the translation of physical forces into changes in membrane potential an essential process for hearing. Any disruption in this mechanism can result in profound hearing impairment due to hair cell dysfunction.

Pathways

TMC1 is central to the mechanotransduction pathway particularly in hair cells. This protein supports the conversion of sound-induced vibrations into neural signals an important process in the auditory pathway. TMC1 closely associates with proteins such as TMIE and LHFPL5 indicating a collective role in ensuring accurate signal transduction. Disturbances in this pathway can undermine the auditory system's ability to interpret external sounds correctly.

TMC1 has significant associations with hearing loss conditions such as DFNA36 a form of autosomal dominant non-syndromic hearing loss and DFNB7/B11 an autosomal recessive hearing impairment. Mutations in TMC1 disrupt its function resulting in defects in the mechanotransduction process in hair cells. This malfunction is often linked to genetic hearing loss where TMC1 mutations lead to deafness through altered interactions with proteins like TMIE and LHFPL5 highlighting the protein's importance in maintaining proper auditory function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pore-forming subunit of the mechanotransducer (MET) non-selective cation channel complex located at the tips of stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system (By similarity). The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, and aided by several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, and the tip-link PCDH15 (By similarity). MET channel is activated by tension in the tip-link extending from the side wall of one stereocilium to the tip of the adjacent shorter stereocilium, where the channel is located (By similarity). TMC1 MET channel is highly permeable to calcium and likely transports monovalent cations (By similarity). Also involved in vestibular hair cells transduction current (By similarity).
See full target information TMC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1213-1227 PubMed40259083

2025

Multi-omic and spatial analysis of mouse kidneys highlights sex-specific differences in gene regulation across the lifespan.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Chen,Ruiyang Liu,Chia-Kuei Mo,Michael C Wendl,Andrew Houston,Preet Lal,Yanyan Zhao,Wagma Caravan,Andrew T Shinkle,Atieh Abedin-Do,Nataly Naser Al Deen,Kazuhito Sato,Xiang Li,André Luiz N Targino da Costa,Yize Li,Alla Karpova,John M Herndon,Maxim N Artyomov,Joshua B Rubin,Sanjay Jain,Xue Li,Sheila A Stewart,Li Ding,Feng Chen
View all publications
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