Rabbit Recombinant Monoclonal TMC2 antibody. Suitable for WB, ICC/IF, IHC - Wmt, Flow Cyt (Intra) and reacts with Transfected cell lysate - Mouse, Mouse, Transfected cell line - Mouse samples.
Images
![Western blot - Anti-TMC2 antibody [EPR19446] (AB200039), expandable thumbnail](https://content.abcam.com/products/images/tmc2-antibody-epr19446-ab200039--western-blot-img13307.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TMC2 antibody [EPR19446] (AB200039)")
![IHC - Wholemount - Anti-TMC2 antibody [EPR19446] (AB200039), expandable thumbnail](https://content.abcam.com/products/images/tmc2-antibody-epr19446-ab200039--ihc-wholemount-img120175.jpg/_jcr_content/renditions/webp-475.webp "IHC - Wholemount - Anti-TMC2 antibody [EPR19446] (AB200039)")
![Immunocytochemistry/ Immunofluorescence - Anti-TMC2 antibody [EPR19446] (AB200039), expandable thumbnail](https://content.abcam.com/products/images/tmc2-antibody-epr19446-ab200039--immunocytochemistry-immunofluorescence-img44802.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TMC2 antibody [EPR19446] (AB200039)")
![Flow Cytometry (Intracellular) - Anti-TMC2 antibody [EPR19446] (AB200039), expandable thumbnail](https://content.abcam.com/products/images/tmc2-antibody-epr19446-ab200039--flow-cytometry-intracellular-img26808.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TMC2 antibody [EPR19446] (AB200039)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC - Wmt | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Mouse | Expected | Expected | Tested | Expected |
| Transfected cell line - Mouse | Not recommended | Tested | Not recommended | Tested |
| Transfected cell lysate - Mouse | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse | Dilution info 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse | Dilution info - | Notes - |
IHC - Wmt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse, Transfected cell line - Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse | Dilution info - | Notes - |
Target data
Function
Pore-forming subunit of the mechanotransducer (MET) non-selective cation channel complex located at the tips of stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system (PubMed:11850618, PubMed:23871232). The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, the tip-link PCDH15, and possibly the PIEZO subunits (By similarity). MET channel is activated by tension in the tip-link extending from the side wall of one stereocilium to the tip of the adjacent shorter stereocilium, where the channel is located (By similarity). TMC2 MET channel is highly permeable to calcium and likely transports monovalent cations (PubMed:23871232). Also involved in vestibular hair cell transduction current of the mammalian inner ear (PubMed:23871232).
Alternative names
Transmembrane channel-like protein 2, Transmembrane cochlear-expressed protein 2, Tmc2
Recommended products
Rabbit Recombinant Monoclonal TMC2 antibody. Suitable for WB, ICC/IF, IHC - Wmt, Flow Cyt (Intra) and reacts with Transfected cell lysate - Mouse, Mouse, Transfected cell line - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19446
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TMC2 or Transmembrane Channel-like 2 is a protein that serves as a component of mechanotransduction channels. It has a mass of approximately 104 kDa. TMC2 is primarily expressed in sensory hair cells of the inner ear playing a critical mechanical function in the conversion of sound vibrations into electrical signals. TMC2 operates by forming part of ion channels that are essential to the sensory perception of auditory stimuli.
- Biological function summary
TMC2 participates in mechanosensory systems playing an important role in hearing. It forms or associates with ion channel complexes that facilitate the flow of ions into sensory cells which is necessary for initiating receptor potentials. Its presence in sensory hair cells indicates its significant role in the auditory transduction process where it likely interacts with other integral membrane proteins to modulate ion channel activity.
- Pathways
Hair cell mechanotransduction involves TMC2 contributing to the mechanotransduction pathway. This pathway is essential for the mechanical-to-electrical sound transduction process. TMC2 likely collaborates with proteins such as TMC1 and other components of the mechanotransduction complex that function in this pathway facilitating ion influx in response to hair bundle deflections caused by sound waves.
- Associated diseases and disorders
Mutations or malfunctions in TMC2 are associated with hearing impairments. Genetic studies indicate its relation to deafness particularly in syndromic and non-syndromic forms of hereditary hearing loss. TMC2's functional relationship with related proteins like TMC1 highlights the critical collaboration between these proteins in maintaining normal auditory function and the risk of auditory disorders when these proteins' functions go awry.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-TMC2 antibody [EPR19446] (ab200039), expandable thumbnail](https://content.abcam.com/products/images/tmc2-antibody-epr19446-ab200039--western-blot-img13307.jpg "Western blot - Anti-TMC2 antibody [EPR19446] (ab200039)")
Western blot - Anti-TMC2 antibody [EPR19446] (ab200039)
Blocking/Dilution buffer: 5% NFDM/TBST.
The Cell lysates were kindly provided by our collaborator Dr. Yun Shi. Nanjing University MARC.
The lysates were not boiled prior to loading on the gel.
All lanes: Western blot - Anti-TMC2 antibody [EPR19446] (ab200039) at 1/5000 dilution
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) transfected with a control vector containing DDDDK tag, whole cell lysate at 10 µg
Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) transfected with DDDDK tagged mouse TMC2 expression vector, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: 101 kDa
Exposure time: 5s
![IHC - Wholemount - Anti-TMC2 antibody [EPR19446] (ab200039), expandable thumbnail](https://content.abcam.com/products/images/tmc2-antibody-epr19446-ab200039--ihc-wholemount-img120175.jpg "IHC - Wholemount - Anti-TMC2 antibody [EPR19446] (ab200039)")
IHC - Wholemount - Anti-TMC2 antibody [EPR19446] (ab200039)
Immunohistochemical analysis of 4% PFA-fixed whole mount P21 mouse cochlea tissue labeling TMC2 with ab200039 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (DyLight 488) secondary antibody at 1/500 dilution (green). The results showed cytoplasmic staining on mouse cochlea inner hair cells.
Red color: F-actin.
The data was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.
![Immunocytochemistry/ Immunofluorescence - Anti-TMC2 antibody [EPR19446] (ab200039), expandable thumbnail](https://content.abcam.com/products/images/tmc2-antibody-epr19446-ab200039--immunocytochemistry-immunofluorescence-img44802.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TMC2 antibody [EPR19446] (ab200039)")
Immunocytochemistry/ Immunofluorescence - Anti-TMC2 antibody [EPR19446] (ab200039)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with DDDDK tagged mouse TMC2 expression vector, labeling TMC2 with ab200039 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HEK-293T cells transfected with DDDDK tagged mouse TMC2 expression vector.
The nuclear counter stain is DAPI (blue). FLAG is detected with Anti-FLAG® M2 (red) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
The negative controls are as follows:
-ve control 1: ab200039 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-FLAG® M2 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Flow Cytometry (Intracellular) - Anti-TMC2 antibody [EPR19446] (ab200039), expandable thumbnail](https://content.abcam.com/products/images/tmc2-antibody-epr19446-ab200039--flow-cytometry-intracellular-img26808.jpg "Flow Cytometry (Intracellular) - Anti-TMC2 antibody [EPR19446] (ab200039)")
Flow Cytometry (Intracellular) - Anti-TMC2 antibody [EPR19446] (ab200039)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK tagged mouse TMC2 expression vector cell line labeling TMC2 with ab200039 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
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