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AB243537

Anti-TMC3 antibody

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(1 Publication)

Rabbit Polyclonal TMC3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TMC3 aa 1 to C-terminus.

View Alternative Names

Transmembrane channel-like protein 3, TMC3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMC3 antibody (AB243537)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMC3 antibody (AB243537)

Formalin-fixed, paraffin-embedded human liver tissue stained for TMC3 with ab243537 at a 1/10000 dilution in immunohistochemical analysis. No positivity observed.

Immunocytochemistry/ Immunofluorescence - Anti-TMC3 antibody (AB243537)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMC3 antibody (AB243537)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling TMC3 using ab243537 at 4 μg/ml (green) in ICC/IF. Nuclear probe is visualized in blue, microtublues in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMC3 antibody (AB243537)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMC3 antibody (AB243537)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for TMC3 with ab243537 at a 1/10000 dilution in immunohistochemical analysis. No positivity observed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMC3 antibody (AB243537)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMC3 antibody (AB243537)

Formalin-fixed, paraffin-embedded human parathyroid gland tissue stained for TMC3 with ab243537 at a 1/10000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMC3 antibody (AB243537)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMC3 antibody (AB243537)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for TMC3 with ab243537 at a 1/10000 dilution in immunohistochemical analysis. No positivity observed.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TMC3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7Z5M5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMC3 also known as Transmembrane Channel-like 3 is a protein encoded by the TMC3 gene in humans. It has a molecular mass of approximately 110 kDa. This protein is part of the transmembrane channel-like family and is primarily expressed in the cochlea and vestibular system of the inner ear. TMC3 is believed to act as a cation channel which is important for the sensory function of both hearing and balance in mammals. The structural configuration allows it to facilitate ion transport across cell membranes.
Biological function summary

TMC3 functions as a component in mechanotransduction processes in the inner ear. As part of the transmembrane channel complex it works collaboratively with other proteins like TMC1 and TMC2 to mediate mechanosensory transduction in hair cells. This activity is essential for converting mechanical stimuli into electrical signals which then travel to the brain as part of auditory and vestibular processing. Though not as well studied as TMC1 the role of TMC3 in this complex suggests it might have stabilizing or supportive functions in the transduction pathway.

Pathways

TMC3 is involved in the mechanosensory signaling pathway within the inner ear. It interacts with other proteins like TMC1 and TMC2 in this pathway to facilitate auditory and balance perception. The pathway includes calcium signaling critical for maintaining hair cell function and communication with the nervous system. TMC3 is not only distinguished by these associations but it also plays a part in synaptic activity important for hearing and balance.

TMC3's disruption may contribute to conditions like hearing loss and vestibular dysfunction. Genetic variations in TMC3 and related proteins such as TMC1 and TMC2 are linked to hereditary forms of hearing impairment. Although research is still ongoing evidence suggests that altered TMC3 expression can impair mechanotransduction leading to sensorineural hearing issues. Understanding TMC3's role could provide insight into therapeutic targets for treating such auditory disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable component of an ion channel (Probable). Molecular function hasn't been characterized yet (Probable).
See full target information TMC3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 53:1579-1589 PubMed34693452

2021

Identification of lipid metabolism-related genes as prognostic indicators in papillary thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shishuai Wen,Yi Luo,Weili Wu,Tingting Zhang,Yichen Yang,Qinghai Ji,Yijun Wu,Rongliang Shi,Ben Ma,Midie Xu,Ning Qu
View all publications
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