Rabbit Polyclonal TMED2 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TMED2 aa 1-100.
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Publications
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- Cancer management and research 11:2203-22142019Increased expression of TMED2 is an unfavorable prognostic factor in patients with breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia Lin et. al.PubMed 31114314
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TMED2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q15363
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase-activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing.
Alternative names
RNP24, TMED2, Transmembrane emp24 domain-containing protein 2, Membrane protein p24A, p24, p24 family protein beta-1, p24beta1
Recommended products
Rabbit Polyclonal TMED2 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TMED2 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TMED2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q15363
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab241213 was affinity purified using an epitope specific to TMED2 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Transmembrane Emp24 protein transport domain containing 2 also known as TMED2 is a protein that plays an important role in the transport of proteins between the endoplasmic reticulum (ER) and the Golgi apparatus. TMED2 weighs approximately 23 kDa. Research indicates TMED2 as highly expressed in various tissues showing a significant presence in the liver and kidney. It acts as part of the p24 family of proteins which are involved in cargo trafficking within intracellular membranes.
- Biological function summary
TMED2 facilitates the sorting and transportation of proteins by forming a coat around vesicles enabling their movement between cellular compartments. It is an essential member of the p24 protein complex which functions as a carrier in the early secretory pathway. This complex supports the maintenance of cellular protein balance and ensures proteins reach their proper destinations efficiently.
- Pathways
TMED2 contributes to the vesicular trafficking pathway which controls the flow of proteins within the cell. It participates in the secretory pathway closely interacting with COPI and COPII proteins that form vesicle coats important for cargo selection and vesicle budding. TMED2 interacts particularly with proteins like TMED10 and TMED9 forming a network that underpins its role in vesical transport from the ER to the Golgi.
- Associated diseases and disorders
TMED2 has connections to certain metabolic disorders and cancer. Improper function or expression of TMED2 can lead to defects in protein trafficking which can disrupt cellular homeostasis and contribute to conditions like congenital disorders of glycosylation. Moreover alterations in TMED2 levels have been observed in some cancer types such as colorectal cancer. It interacts with cancer-related proteins such as GRP94 influencing tumor progression and metastasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-TMED2 antibody (ab241213)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-TMED2 antibody (ab241213) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg
Lane 5: NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 30s
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