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AB251705

Anti-TMED2 antibody

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(3 Publications)

Rabbit Polyclonal TMED2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TMED2 aa 1-150.

View Alternative Names

RNP24, TMED2, Transmembrane emp24 domain-containing protein 2, Membrane protein p24A, p24, p24 family protein beta-1, p24beta1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-TMED2 antibody (AB251705)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TMED2 antibody (AB251705)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TMED2 (green) using ab251705 at 4 µg/ml in ICC/IF.

Western blot - Anti-TMED2 antibody (AB251705)
  • WB

Unknown

Western blot - Anti-TMED2 antibody (AB251705)

All lanes:

Western blot - Anti-TMED2 antibody (ab251705) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 22 kDa

false

Western blot - Anti-TMED2 antibody (AB251705)
  • WB

Unknown

Western blot - Anti-TMED2 antibody (AB251705)

All lanes:

Western blot - Anti-TMED2 antibody (ab251705) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat cell line) cell lysate

Predicted band size: 22 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMED2 antibody (AB251705)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMED2 antibody (AB251705)

Paraffin-embedded human cerebral cortex tissue stained for TMED2 using ab251705 at 1/20 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TMED2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q15363

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transmembrane Emp24 protein transport domain containing 2 also known as TMED2 is a protein that plays an important role in the transport of proteins between the endoplasmic reticulum (ER) and the Golgi apparatus. TMED2 weighs approximately 23 kDa. Research indicates TMED2 as highly expressed in various tissues showing a significant presence in the liver and kidney. It acts as part of the p24 family of proteins which are involved in cargo trafficking within intracellular membranes.
Biological function summary

TMED2 facilitates the sorting and transportation of proteins by forming a coat around vesicles enabling their movement between cellular compartments. It is an essential member of the p24 protein complex which functions as a carrier in the early secretory pathway. This complex supports the maintenance of cellular protein balance and ensures proteins reach their proper destinations efficiently.

Pathways

TMED2 contributes to the vesicular trafficking pathway which controls the flow of proteins within the cell. It participates in the secretory pathway closely interacting with COPI and COPII proteins that form vesicle coats important for cargo selection and vesicle budding. TMED2 interacts particularly with proteins like TMED10 and TMED9 forming a network that underpins its role in vesical transport from the ER to the Golgi.

TMED2 has connections to certain metabolic disorders and cancer. Improper function or expression of TMED2 can lead to defects in protein trafficking which can disrupt cellular homeostasis and contribute to conditions like congenital disorders of glycosylation. Moreover alterations in TMED2 levels have been observed in some cancer types such as colorectal cancer. It interacts with cancer-related proteins such as GRP94 influencing tumor progression and metastasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase-activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing.
See full target information TMED2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 22:5 PubMed39958879

2025

Relationship between transmembrane emp24 domain containing 2 expression and tumor stem cell characteristics in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhui Wu,Shunchu Zhang,Guimei Zou

Cell cycle (Georgetown, Tex.) 21:2403-2416 PubMed35833665

2022

Linc00883 affects colorectal cancer through miR-577/FKBP14 axis: a novel mechanism for regulating colorectal cancer cell proliferation, invasion, and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Bai,Ling Li,Zhiyong Zhang

World journal of surgical oncology 20:32 PubMed35135563

2022

Transmembrane p24 trafficking protein 2 regulates inflammation through the TLR4/NF-κB signaling pathway in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Longhua Feng,Pengjiang Cheng,Zhengyun Feng,Xiaoyu Zhang
View all publications
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