Rabbit Polyclonal TMED2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TMED2 aa 1-150.
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Publications
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- World journal of surgical oncology 20:322022Transmembrane p24 trafficking protein 2 regulates inflammation through the TLR4/NF-κB signaling pathway in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLonghua Feng,Pengjiang Cheng,Zhengyun Feng,Xiaoyu ZhangPubMed 35135563
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TMED2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q15363
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Chinese hamster | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info - | Notes - |
Target data
Function
Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase-activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing.
Alternative names
RNP24, TMED2, Transmembrane emp24 domain-containing protein 2, Membrane protein p24A, p24, p24 family protein beta-1, p24beta1
Recommended products
Rabbit Polyclonal TMED2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TMED2 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TMED2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q15363
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Transmembrane Emp24 protein transport domain containing 2 also known as TMED2 is a protein that plays an important role in the transport of proteins between the endoplasmic reticulum (ER) and the Golgi apparatus. TMED2 weighs approximately 23 kDa. Research indicates TMED2 as highly expressed in various tissues showing a significant presence in the liver and kidney. It acts as part of the p24 family of proteins which are involved in cargo trafficking within intracellular membranes.
- Biological function summary
TMED2 facilitates the sorting and transportation of proteins by forming a coat around vesicles enabling their movement between cellular compartments. It is an essential member of the p24 protein complex which functions as a carrier in the early secretory pathway. This complex supports the maintenance of cellular protein balance and ensures proteins reach their proper destinations efficiently.
- Pathways
TMED2 contributes to the vesicular trafficking pathway which controls the flow of proteins within the cell. It participates in the secretory pathway closely interacting with COPI and COPII proteins that form vesicle coats important for cargo selection and vesicle budding. TMED2 interacts particularly with proteins like TMED10 and TMED9 forming a network that underpins its role in vesical transport from the ER to the Golgi.
- Associated diseases and disorders
TMED2 has connections to certain metabolic disorders and cancer. Improper function or expression of TMED2 can lead to defects in protein trafficking which can disrupt cellular homeostasis and contribute to conditions like congenital disorders of glycosylation. Moreover alterations in TMED2 levels have been observed in some cancer types such as colorectal cancer. It interacts with cancer-related proteins such as GRP94 influencing tumor progression and metastasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-TMED2 antibody (ab251705)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TMED2 (green) using ab251705 at 4 µg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMED2 antibody (ab251705)
Paraffin-embedded human cerebral cortex tissue stained for TMED2 using ab251705 at 1/20 dilution in immunohistochemical analysis.
Western blot - Anti-TMED2 antibody (ab251705)
All lanes: Western blot - Anti-TMED2 antibody (ab251705) at 0.4 µg/mL
Lane 1: RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG (Human brain glioma cell line) cell lysate
Predicted band size: 22 kDa
Western blot - Anti-TMED2 antibody (ab251705)
All lanes: Western blot - Anti-TMED2 antibody (ab251705) at 0.4 µg/mL
Lane 1: NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2: NBT-II (Rat cell line) cell lysate
Predicted band size: 22 kDa
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