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Rabbit Recombinant Monoclonal T106B antibody. Suitable for WB, ICC/IF and reacts with Recombinant fragment - Human, Human, Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-TMEM106B antibody [EPR27525-70] (AB321970), expandable thumbnail
  • Western blot - Anti-TMEM106B antibody [EPR27525-70] (AB321970), expandable thumbnail
  • Western blot - Anti-TMEM106B antibody [EPR27525-70] (AB321970), expandable thumbnail
  • Western blot - Anti-TMEM106B antibody [EPR27525-70] (AB321970), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-FrIHC-P
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

In neurons, involved in the transport of late endosomes/lysosomes (PubMed:25066864). May be involved in dendrite morphogenesis and maintenance by regulating lysosomal trafficking (PubMed:25066864). May act as a molecular brake for retrograde transport of late endosomes/lysosomes, possibly via its interaction with MAP6 (By similarity). In motoneurons, may mediate the axonal transport of lysosomes and axonal sorting at the initial segment (By similarity). It remains unclear whether TMEM106B affects the transport of moving lysosomes in the anterograde or retrograde direction in neurites and whether it is important in the sorting of lysosomes in axons or in dendrites (By similarity). In neurons, may also play a role in the regulation of lysosomal size and responsiveness to stress (PubMed:25066864). Required for proper lysosomal acidification (By similarity). (Microbial infection) Plays a role in human coronavirus SARS-CoV-2 infection, but not in common cold coronaviruses HCoV-229E and HCoV-OC43 infections. Involved in ACE2-independent SARS-CoV-2 cell entry. Required for post-endocytic stage of virus entry, facilitates spike-mediated membrane fusion. Virus attachment and endocytosis can also be mediated by other cell surface receptors.

Alternative names

Transmembrane protein 106B, TMEM106B

Recommended products

Rabbit Recombinant Monoclonal T106B antibody. Suitable for WB, ICC/IF and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27525-70
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for mouse ICC.
This antibody cross-react with recombinant human TMEM106C by western blot.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TMEM106B also known as transmembrane protein 106B is a type II single-pass transmembrane protein with a molecular mass of approximately 33 kDa. It is expressed in specific regions of the brain as well as in other tissue types such as the lungs and immune system cells. Its expression levels are especially elevated in microglia and oligodendrocytes which are brain cells that play important roles in immune response and myelination respectively. Structurally TMEM106B contains a transmembrane domain and its precise biochemical function is still under investigation though it is thought to participate in lysosomal and vesicular trafficking.

Biological function summary

TMEM106B is involved in several cellular processes impacting lysosome function and trafficking. It may become part of a protein complex that manages lysosomal positioning and pathway regulation within the cell. TMEM106B also affects cellular homeostasis and protein degradation which suggests a role in maintaining the proper balance of proteins and lipids within lysosomes. Alteration in TMEM106B expression or function can cause changes in lysosomal size and distribution indicating its role in cellular logistics.

Pathways

The TMEM106B protein becomes involved in the endolysosomal pathway and the autophagy-lysosome pathway. These pathways are critical for processing and breakdown of cellular waste and recycling of cellular components. In these pathways TMEM106B interacts with other proteins such as SORT1 (sortilin 1) and GRN (progranulin) both of which have important roles in protein sorting and trafficking. By influencing these pathways TMEM106B helps maintain cellular health and function.

Associated diseases and disorders

TMEM106B links to neurodegenerative conditions such as frontotemporal lobar degeneration and possibly Alzheimer's disease. Mutations or abnormal expression levels of TMEM106B have been implicated in the pathogenesis and progression of these diseases. In frontotemporal lobar degeneration TMEM106B interacts with proteins like GRN where changes can lead to neurodegeneration. In Alzheimer's disease abnormal lysosomal function tied to TMEM106B may contribute to the accumulation of pathogenic proteins.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-TMEM106B antibody [EPR27525-70] (ab321970), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TMEM106B antibody [EPR27525-70] (ab321970)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling TMEM106B with ab321970 at 1/500 (1.034 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in A549 cell line (shown in green) and no staining in TMEM106B KO A549 cell line. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-TMEM106B antibody [EPR27525-70] (ab321970), expandable thumbnail

    Western blot - Anti-TMEM106B antibody [EPR27525-70] (ab321970)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody cross-react with recombinant human TMEM106C by western blot.

    All lanes: Western blot - Anti-TMEM106B antibody [EPR27525-70] (ab321970) at 1/1000 dilution

    Lane 1: His-tagged human TMEM106B recombinant fragment at 10 ng

    Lane 2: His-tagged human TMEM106C recombinant fragment at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 14 kDa

    Exposure time: 26s

  • Western blot - Anti-TMEM106B antibody [EPR27525-70] (ab321970), expandable thumbnail

    Western blot - Anti-TMEM106B antibody [EPR27525-70] (ab321970)

    Blocking buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS.

    Diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBST

    The samples were run on a Bis-Tris gel under reducing conditions.

    Western blot: Anti-TMEM106B antibody (ab321970) staining at 1/1000 dilution, shown in green; Mouse anti-Tubulin antibody [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta.

    In Western blot, ab321970 was shown to bind specifically to TMEM106B. Target of interest was observed at 40 kDa in wild-type A549 cell lysates (lane 1) with no signal observed at this size in TMEM106B knockout cell line (lane 2, knockout cell line Human TMEM106B knockout A549 cell line ab273711 / knockout cell lysate Human TMEM106B knockout A549 cell lysate ab273769). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.

    Negative control: Raji.

    Exposure time: N/A

    All lanes: Western blot - Anti-TMEM106B antibody [EPR27525-70] (ab321970) at 1/1000 dilution

    Lane 1: Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: TMEM106B knockout A549 whole cell lysate at 20 µg

    Lane 3: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 40 kDa

  • Western blot - Anti-TMEM106B antibody [EPR27525-70] (ab321970), expandable thumbnail

    Western blot - Anti-TMEM106B antibody [EPR27525-70] (ab321970)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22895706).

    All lanes: Western blot - Anti-TMEM106B antibody [EPR27525-70] (ab321970) at 1/1000 dilution

    Lane 1: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 2: 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: Mouse testis tissue lysate at 20 µg

    Lane 5: Mouse kidney tissue lysate at 20 µg

    Lane 6: Mouse pancreas tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Exposure time: 81s

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Product protocols

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