Anti-TMEM106B antibody [EPR30569-128] - N-terminal

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Immunocytochemistry/ Immunofluorescence - Anti-TMEM106B antibody [EPR30569-128] - N-terminal (AB325555)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty expression vector containing a myc-His-tag®; 293T cell transfected with a human TMEM106B expression vector containing a myc-His-tag®; 293T cell transfected with a human TMEM106C expression vector containing a myc-His-tag®; 293T cells transfected with a human TMEM106A expression vector containing a myc-His-tag® cells labelling TMEM106B with ab325555 at 1/8000 (0.062 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells (shown in green) transfected with a human TMEM106B expression vector containing a myc-His-tag®, showing no staining in 293T cells transfected with a human TMEM106C/A or empty expression vector containing a myc-His-tag®The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Panel A : 293T cells transfected with an empty expression vector containing a myc-His-tag®.

Panel B : 293T cells transfected with a human TMEM106B expression vector containing a myc-His-tag®.

Panel C : 293T cells transfected with a human TMEM106C expression vector containing a myc-His-tag®.

Panel D : 293T cells transfected with a human TMEM106A expression vector containing a myc-His-tag®.

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain Myc at 1/100 dilution (Magenta).

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Immunocytochemistry/ Immunofluorescence - Anti-TMEM106B antibody [EPR30569-128] - N-terminal (AB325555)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized PANC-1(human pancreatic epithelioid carcinoma epithelial cell); Raji (human Burkitt's lymphoma B lymphocyte) cells labelling TMEM106B with ab325555 at 1/500 (0.998 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing lysosome staining in PANC-1 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control : Raji.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab25631 Anti-LAMP2 mouse monoclonal antibody - Lysosome Marker was used to counterstain lysosomes at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Magenta).

-ve control 1 : ab325555 at a 1/500 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab25631 at a 1/200 dilution followed by ab150081 at a 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Anti-TMEM106B antibody [EPR30569-128] - N-terminal (AB325555)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Wildtype A549 (wildtype human lung carcinoma epithelial cell); TMEM106B KO A549 (TMEM106B knockout A549), ab273711 cells labelling TMEM106B with ab325555 at 1/500 (0.998 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing lysosome staining in wildtype A549 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab25631 Anti-LAMP2 mouse monoclonal antibody - Lysosome Marker was used to counterstain lysosomes at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Anti-TMEM106B antibody [EPR30569-128] - N-terminal (AB325555)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling TMEM106B with ab325555 at 1/500 (0.998 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing lysosome staining in 3T3-L1 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab25631 Anti-LAMP2 mouse monoclonal antibody - Lysosome Marker was used to counterstain lysosomes at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Magenta).

-ve control 1 : ab325555 at a 1/500 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab25631 at a 1/200 dilution followed by ab150081 at a 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Anti-TMEM106B antibody [EPR30569-128] - N-terminal (AB325555)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling TMEM106B with ab325555 at 1/500 (0.998 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing lysosome staining in mouse primary neural/glia cells (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Panel A : anti-TMEM106B stained on mouse primary neural/glia cells.

Panel B : merged staining of anti-TMEM106B (ab325555, green), anti-LAMP1 (magenta), anti-MAP2 (ab11267, white) on mouse primary neural/glia cells.

Panel C : anti-LAMP1 stained on mouse primary neural/glia cells.

Panel D : anti-MAP2 stained in neurons of mouse primary neural/glia cells.

ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain neurons at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Magenta).