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AB241992

Anti-TMEM109/Mitsugumin-23 antibody

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(1 Publication)

Rabbit Polyclonal TMEM109/Mitsugumin-23 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TMEM109 aa 1-100.

View Alternative Names

Voltage-gated monoatomic cation channel TMEM109, Mitsugumin-23, Transmembrane protein 109, Mg23, TMEM109

2 Images
Immunoprecipitation - Anti-TMEM109/Mitsugumin-23 antibody (AB241992)
  • IP

Supplier Data

Immunoprecipitation - Anti-TMEM109/Mitsugumin-23 antibody (AB241992)

TMEM109/Mitsugumin-23 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241992 at 20 μL per reaction. Western blot was performed from the immunoprecipitate using ab241992 at 1/100 dilution.

Lane 1 : ab241992 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

Prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-TMEM109/Mitsugumin-23 antibody (ab241992)

Predicted band size: 26 kDa

false

Western blot - Anti-TMEM109/Mitsugumin-23 antibody (AB241992)
  • WB

Supplier Data

Western blot - Anti-TMEM109/Mitsugumin-23 antibody (AB241992)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-TMEM109/Mitsugumin-23 antibody (ab241992) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 26 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TMEM109 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9BVC6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to TMEM109/Mitsugumin-23 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM109 also known as Mitsugumin-23 (MG23) functions as a transmembrane protein with a mass of approximately 20-25 kDa. It resides in the endoplasmic reticulum membrane where it acts as an important component in maintaining cellular homeostasis. This protein is primarily observed across various tissues with a higher expression in muscle tissue and certain types of epithelial cells. Its structure suggests roles in calcium signaling due to domains that allow ion passage across cellular membranes.
Biological function summary

TMEM109 plays a role in the regulation of calcium ion concentrations within cells contributing to the mitochondrial permeability transition pore (MPTP) complex. This complex controls mitochondrial function by regulating the passage of ions and small metabolites which is critical for cell survival and apoptosis. Through these activities TMEM109 influences processes like muscle contraction and cell death highlighting its significance in cellular energy management and programmed cell death pathways.

Pathways

TMEM109 has roles in calcium signaling and apoptotic pathways two significant biological processes for cellular health. In the calcium signaling pathway it interacts with other proteins such as SERCA1 (Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 1) to help manage calcium ion levels within cells. In apoptotic pathways TMEM109's involvement in the MPTP complex links it to the BAX protein which is an essential regulator of apoptosis facilitating the release of cytochrome c from mitochondria triggering cascade events leading to cell death.

Irregular function or expression of TMEM109 associates with conditions such as muscular dystrophy and neurodegenerative disorders. For example in muscular dystrophy altered calcium regulation and signaling through MG23 may impact muscle contraction and integrity. Additionally abnormal TMEM109 activity can connect to Alzheimer’s disease where disrupted calcium homeostasis and apoptotic signaling play roles. These disease connections make TMEM109 a considerable target for therapeutic research and intervention often intersecting pathways with proteins like dystrophin in muscular dystrophy cases and amyloid precursor protein (APP) in Alzheimer's disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a voltage-gated monoatomic cation channel permeable to both potassium and calcium (By similarity). Plays a role in the cellular response to DNA damage (PubMed : 23542032).
See full target information TMEM109
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine : PubMed32757436

2020

Quantitative proteomics identified 3 oxidative phosphorylation genes with clinical prognostic significance in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Su,Fen-Fang Zhou,Tao Zhang,Dan-Wen Wang,Da Zhao,Xiao-Ming Hou,Mao-Hui Feng
View all publications
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Product promise

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