Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405) is a rabbit monoclonal antibody detecting TMEM119 in Western Blot, Flow Cytometry. Suitable for Mouse.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
Images
![Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (AB210405), expandable thumbnail](https://content.abcam.com/products/images/tmem119-antibody-106-6-microglial-marker-ab210405--flow-cytometry-img132219.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (AB210405)")
![Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (AB210405), expandable thumbnail](https://content.abcam.com/products/images/tmem119-antibody-106-6-microglial-marker-ab210405--flow-cytometry-img81029.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (AB210405)")
![Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (AB210405), expandable thumbnail](https://content.abcam.com/products/images/tmem119-antibody-106-6-microglial-marker-ab210405--western-blot-img435365.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (AB210405)")
Publications
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- Biomolecules 12:2022Advances in Visualizing Microglial Cells in Human Central Nervous System Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher E G Uff,Karishma Patel,Charming Yeung,Ping K YipPubMed 35625531
- Frontiers in immunology 12:6867692021A Population of M2 Macrophages Associated With Bone Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth Olmsted-Davis,Julio Mejia,Elizabeth Salisbury,Zbigniew Gugala,Alan R DavisPubMed 34712222
- Drug design, development and therapy 15:1717-17242021Alendronate Alleviated Femoral Head Necrosis and Upregulated BMP2/EIF2AK3/EIF2A/ATF4 Pathway in Liquid Nitrogen Treated Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Rong,Xiaoliu Li,Weimin Jiang,Xuhua Wu,Qingquan Xia,Jie Chen,Xiaofan YinPubMed 33935494
- Nature communications 12:11512021Single-cell RNA sequencing reveals functional heterogeneity of glioma-associated brain macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia Ochocka,Pawel Segit,Kacper Adam Walentynowicz,Kamil Wojnicki,Salwador Cyranowski,Julian Swatler,Jakub Mieczkowski,Bozena KaminskaPubMed 33608526
- Immunity 54:484-498.e82021Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin M.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Elizabeth Deerhake,Keiko Danzaki,Makoto Inoue,Emre D Cardakli,Toshiaki Nonaka,Nupur Aggarwal,William E Barclay,Ru-Rong Ji,Mari L ShinoharaPubMed 33581044
- Nature neuroscience 23:167-1712019Microglial microRNAs mediate sex-specific responses to tau pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLay Kodama,Elmer Guzman,Jon I Etchegaray,Yaqiao Li,Faten A Sayed,Lu Zhou,Yungui Zhou,Lihong Zhan,David Le,Joe C Udeochu,Claire D Clelland,Zuolin Cheng,Guoqiang Yu,Qingyun Li,Kenneth S Kosik,Li GanPubMed 31873194
- Nature communications 10:38902019Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia P Figueiredo et. al.PubMed 31488835
- Frontiers in immunology 10:14672019Indispensable Role of CXCR1 Dendritic Cells in Regulation of Virus-Induced Neuroinflammation Through Rapid Development of Antiviral Immunity in Peripheral Lymphoid Tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Young Choi et. al.PubMed 31316515
- Cytometry. Part A : the journal of the International Society for Analytical Cytology 95:422-4262019OMIP-054: Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass Cytometry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie A Dusoswa,Jan Verhoeff,Juan J Garcia-VallejoPubMed 30701669
- Neuron 101:207-223.e102019Developmental Heterogeneity of Microglia and Brain Myeloid Cells Revealed by Deep Single-Cell RNA Sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingyun Li,Zuolin Cheng,Lu Zhou,Spyros Darmanis,Norma F Neff,Jennifer Okamoto,Gunsagar Gulati,Mariko L Bennett,Lu O Sun,Laura E Clarke,Julia Marschallinger,Guoqiang Yu,Stephen R Quake,Tony Wyss-Coray,Ben A BarresPubMed 30606613
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | IHC-P | IHC-Fr | WB | |
|---|---|---|---|---|
| Human | Expected | Not recommended | Not recommended | Tested |
| Mouse | Tested | Not recommended | Not recommended | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.10000-0.50000 µg/mL | Notes For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474 |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Plays an important role in bone formation and normal bone mineralization (PubMed:20025746, PubMed:22416756, PubMed:26207632). Promotes the differentiation of myoblasts into osteoblasts (PubMed:20025746, PubMed:22416756, PubMed:22579779). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway (PubMed:21239498, PubMed:22579779). Up-regulates the expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Essential for normal spermatogenesis and late testicular differentiation (PubMed:26207632).
Alternative names
Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119
Recommended products
Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405) is a rabbit monoclonal antibody detecting TMEM119 in Western Blot, Flow Cytometry. Suitable for Mouse.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 106-6
- Purification technique
- Affinity purification Protein A
- Specificity
Human reactivity has not been tested.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt) in Mouse samples.
What is the molecular weight of TMEM119?
Anti-TMEM119 [106-6] - Microglial marker (ab210405) specifically detects a band for TMEM119 (UniProt: Q8R138) at a molecular weight of 29kDa.
Trusted by the scientific community
Anti-TMEM119 [106-6] - Microglial marker (ab210405) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.
Reviewed by scientists
Anti-TMEM119 [106-6] - Microglial marker (ab210405) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405) has been confirmed by Flow Cytometry testing in Tmem119 Knockout Mouse microglia samples.
Other related products
We have a range of other formats of antibody clone [106-6] also available for your convenience:
ab210405, Carrier free - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free ab220249, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] - Microglial marker ab225494, PE - PE Anti-TMEM119 antibody [106-6] - Microglial marker ab225496, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker ab225497
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.
- Biological function summary
TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.
- Pathways
TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.
- Associated diseases and disorders
TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405), expandable thumbnail](https://content.abcam.com/products/images/tmem119-antibody-106-6-microglial-marker-ab210405--flow-cytometry-img132219.jpg "Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)")
Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)
Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged mouse TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.
Alexa Fluor® 488 (Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker ab225497) and R-PE (PE Anti-TMEM119 antibody [106-6] - Microglial marker ab225496) conjugated versions are available for this clone.
![Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405), expandable thumbnail](https://content.abcam.com/products/images/tmem119-antibody-106-6-microglial-marker-ab210405--flow-cytometry-img81029.jpg "Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)")
Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)
Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.
No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).
The data was provided by Ben Barres' lab (Stanford University). PMID: 26884166.
![Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405), expandable thumbnail](https://content.abcam.com/products/images/tmem119-antibody-106-6-microglial-marker-ab210405--western-blot-img435365.jpg "Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)")
Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)
Western blot: Anti-TMEM119 antibody [106-6] (ab210405) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab210405 was shown to bind specifically to TMEM119. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405) at 1/1000 dilution
Lane 1: HEK-293T overexpressing TMEM119 OE-1160 cell lysate at 20 µg
Lane 2: HEK-293T cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 40 kDa, 43 kDa
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