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Rabbit Recombinant Monoclonal TMEM119 antibody. Microglia marker. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 16 publications.


Images

Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (AB210405), expandable thumbnail
  • Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (AB210405), expandable thumbnail
  • Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (AB210405), expandable thumbnail

Publications

  • Biomolecules 12:2022
    Advances in Visualizing Microglial Cells in Human Central Nervous System Tissue.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Christopher E G Uff,Karishma Patel,Charming Yeung,Ping K Yip
    PubMed 35625531
  • Frontiers in immunology 12:6867692021
    A Population of M2 Macrophages Associated With Bone Formation.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Elizabeth Olmsted-Davis,Julio Mejia,Elizabeth Salisbury,Zbigniew Gugala,Alan R Davis
    PubMed 34712222

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-PIHC-FrWB
Human
Expected
Not recommended
Not recommended
Tested
Mouse
Tested
Not recommended
Not recommended
Expected

Tested
Tested

Species

Mouse

Dilution info

0.10000-0.50000 µg/mL

Notes

For detailed protocol of microglia extraction from mouse brain, spinal cord or retina, please refer to PMID 26884166 and PMID 28963474

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

    4 products for Alternative Version

      Target data

      Function

      Plays an important role in bone formation and normal bone mineralization (PubMed:26207632, PubMed:22416756, PubMed:20025746). Promotes the differentiation of myoblasts into osteoblasts (PubMed:22416756, PubMed:20025746, PubMed:22579779). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway (PubMed:21239498, PubMed:22579779). Upregulates the expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Essential for normal spermatogenesis and late testicular differentiation (PubMed:26207632).

      Alternative names

      Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119

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      Rabbit Recombinant Monoclonal TMEM119 antibody. Microglia marker. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 16 publications.

      Alternative names

      Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      106-6

      Purification technique

      Affinity purification Protein A

      Specificity

      Human reactivity has not been tested.

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Please note, this antibody is suitable for flow cytometry. For IHC on mouse brain tissue we recommend Anti-TMEM119 antibody [28-3] - Microglial marker ab209064.

      Please note that the original Bennett et al. (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author's permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.

      The 106-6 clone to mouse Tmem119 is exclusively manufactured and sold by Abcam.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.

      Biological function summary

      TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.

      Pathways

      TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.

      Associated diseases and disorders

      TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      3 product images

      • Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405), expandable thumbnail

        Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)

        Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged mouse TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.

        Alexa Fluor® 488 (Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] ab225497) and R-PE (PE Anti-TMEM119 antibody [106-6] ab225496) conjugated versions are available for this clone.

      • Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405), expandable thumbnail

        Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)

        Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.

        No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).

        The data was provided by Ben Barres' lab (Stanford University).

      • Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405), expandable thumbnail

        Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405)

        Western blot: Anti-TMEM119 antibody [106-6] (ab210405) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab210405 was shown to bind specifically to TMEM119. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

        All lanes: Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (ab210405) at 1/1000 dilution

        Lane 1: HEK-293T overexpressing TMEM119 OE-1160 cell lysate at 20 µg

        Lane 2: HEK-293T cell lysate at 20 µg

        Secondary

        All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

        Performed under reducing conditions.

        Observed band size: 40 kDa, 43 kDa

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com