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AB220249

Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free

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(1 Review)

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(2 Publications)

Rabbit Recombinant Monoclonal TMEM119 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119

7 Images
Flow Cytometry (Intracellular) - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)

Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (Alexa Fluor® 647). Please refer to ab225494 for protocol details.

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225494 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 647 ab199093 (left). Cells were surface-stained with ab225494, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 488 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

Flow Cytometry (Intracellular) - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)

Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (Alexa Fluor® 488). Please refer to ab225497 for protocol details.

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225497 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Alexa Fluor® 488 ab199091 (left). Cells were surface-stained with ab225497, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

Flow Cytometry (Intracellular) - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)

Clone 106-6 (ab220249) has been successfully conjugated by Abcam. This image was generated using Anti-TMEM119 antibody [106-6] (PE). Please refer to ab225496 for protocol details.

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225496 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Phycoerythrin ab209478 (left). Cells were surface-stained with ab225496, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)
  • Flow Cyt

Lab

Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged mouse TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Cells were surface-stained with ab210405, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody and Alexa Fluor® 488 conjugated secondary antibody. Only Myc-tag (+) population showed TMEM119 positive staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210405).

Immunocytochemistry/ Immunofluorescence - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)

This data was developed using ab210405, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling TMEM119 with ab210405 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) antibody at 1/1000 dilution.

Confocal image showing cytoplasmic staining in SH-SY5Y cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control : U-937.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution (Magenta).

Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution.

Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)
  • Flow Cyt

Collaborator

Flow Cytometry - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)

Flow cytometric analysis of acutely isolated primary mouse microglia (P60 BL6 mouse; wildtype CD11b+CD45lo brain cells) cells  labeling TMEM119 with ab210405 at 0.5μg/mL (red) and 0.1μg/mL (blue), compared with TMEM119 KO primary mouse brain cells (black) stained with ab210405 at 0.5μg/mL. Goat anti-Rabbit IgG (Alexa Fluor®488) at 1/500 dilution was used as the secondary antibody.

No signal was detected on the surface of CD11b+CD45lo brain cells from TMEM119 KO mouse (black) stained with ab210405; whereas in wildtype CD11b+CD45lo brain cells, cell surface staining was observed (red 0.5ug/mL; blue 0.1ug/mL).

The data was provided by Ben Barres’ lab (Stanford University). PMID : 26884166.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210405).

Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)
  • WB

Lab

Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker - BSA and Azide free (AB220249)

This data was developed using the same antibody clone in a different buffer formulation (ab210405).

Western blot : Anti-TMEM119 antibody [106-6] (ab210405) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab210405 was shown to bind specifically to TMEM119. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-TMEM119 antibody [106-6] - Microglial marker (<a href='/en-us/products/primary-antibodies/tmem119-antibody-106-6-microglial-marker-ab210405'>ab210405</a>) at 1/1000 dilution

Lane 1:

HEK-293T overexpressing TMEM119 OE-1160 cell lysate at 20 µg

Lane 2:

HEK-293T cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 40 kDa,43 kDa

false

  • Unconjugated

    Anti-TMEM119 antibody [106-6] - Microglial marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TMEM119 antibody [106-6] - Microglial marker

  • 578 PE

    PE Anti-TMEM119 antibody [106-6] - Microglial marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

106-6

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

ICC/IF, Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Human reactivity has not been tested.

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Reactivity data

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Product details

ab220249 is the carrier-free version of ab210405.

Please note that the original Bennett et al . (2016) publication (PubMed: 26884166), used a combination of clones 106-6 and 85-5. With the author's permission, the decision was made to add the recombinant version of only a single clone (106-6) to the catalogue as it performed equally well on its own.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.
Biological function summary

TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.

Pathways

TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.

TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in bone formation and normal bone mineralization (PubMed : 20025746, PubMed : 22416756, PubMed : 26207632). Promotes the differentiation of myoblasts into osteoblasts (PubMed : 20025746, PubMed : 22416756, PubMed : 22579779). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway (PubMed : 21239498, PubMed : 22579779). Up-regulates the expression of ATF4 which plays a central role in osteoblast differentiation (PubMed : 24362451). Essential for normal spermatogenesis and late testicular differentiation (PubMed : 26207632).
See full target information Tmem119
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:47 PubMed39988657

2025

Astroglial modulation of synaptic function in the non-demyelinated cerebellar cortex is dependent on MyD88 signaling in a model of toxic demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie Lohrberg,Lena Sünke Mortensen,Carolina Thomas,Franziska Fries,Franziska van der Meer,Alexander Götz,Carolin Landt,Hong Jun Rhee,JeongSeop Rhee,David Gómez-Varela,Manuela Schmidt,Wiebke Möbius,Torben Ruhwedel,Luis A Pardo,Linus Remling,Nadine Kramann,Claudia Wrzos,Erik Bahn,Christine Stadelmann,Alonso Barrantes-Freer

Proceedings of the National Academy of Sciences of 113:E1738-46 PubMed26884166

2016

New tools for studying microglia in the mouse and human CNS.

Applications

Unspecified application

Species

Unspecified reactive species

Mariko L Bennett,F Chris Bennett,Shane A Liddelow,Bahareh Ajami,Jennifer L Zamanian,Nathaniel B Fernhoff,Sara B Mulinyawe,Christopher J Bohlen,Aykezar Adil,Andrew Tucker,Irving L Weissman,Edward F Chang,Gordon Li,Gerald A Grant,Melanie G Hayden Gephart,Ben A Barres
View all publications
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