Anti-TMEM119 antibody [28-3] - Microglial marker is a rabbit recombinant monoclonal antibody that is used to detect TMEM119 in IHC-FoFr, IHC-Fr, IHC-P. Suitable for Mouse samples.
- Recognises Mouse TMEM119, a highly specific microglia marker not expressed by macrophages, other immune or neural cell types
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 135 publications
pH: 7.2 - 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
IHC-FoFr | IHC-P | IHC-Fr | |
---|---|---|---|
Human | Not recommended | Not recommended | Not recommended |
Mouse | Tested | Tested | Tested |
Rat | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1-5 µg/mL | Notes Perform heat mediated antigen retrieval with citrate buffer pH 6. Incubate the section with primary antibody at 4 ℃ overnight. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.1-0.5 µg/mL | Notes Tris-EDTA buffer preferred. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes Tris-EDTA buffer preferred. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Tris-EDTA buffer preferred. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1-5 µg/mL | Notes We recommend using 0.3-0.5% Triton X-100. Perform heat mediated antigen retrieval before IHC-Fr staining protocol, if the signal is too weak. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes We recommend using 0.3-0.5% Triton X-100. Perform heat mediated antigen retrieval before IHC-Fr staining protocol, if the signal is too weak. |
Species Human | Dilution info - | Notes We recommend using 0.3-0.5% Triton X-100. Perform heat mediated antigen retrieval before IHC-Fr staining protocol, if the signal is too weak. |
Select an associated product type
Plays an important role in bone formation and normal bone mineralization (PubMed:20025746, PubMed:22416756, PubMed:26207632). Promotes the differentiation of myoblasts into osteoblasts (PubMed:20025746, PubMed:22416756, PubMed:22579779). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway (PubMed:21239498, PubMed:22579779). Up-regulates the expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Essential for normal spermatogenesis and late testicular differentiation (PubMed:26207632).
Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119
Anti-TMEM119 antibody [28-3] - Microglial marker is a rabbit recombinant monoclonal antibody that is used to detect TMEM119 in IHC-FoFr, IHC-Fr, IHC-P. Suitable for Mouse samples.
- Recognises Mouse TMEM119, a highly specific microglia marker not expressed by macrophages, other immune or neural cell types
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 135 publications
pH: 7.2 - 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
This antibody recognizes mouse Tmem119, a transmembrane protein that has been reported to be a highly specific microglia marker that is not expressed by macrophages or other immune or neural cell types (Bennett et al., 2016).
FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the support & downloads section.
Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-FoFr, IHC-Fr and IHC-P
Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064) was first used in a scientific publication in 2016 and has been cited over 139 times in peer reviewed journals. It's performance in IHC and Immunofluorescence in mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064) has been confirmed by IHC testing in TMEM119 knockout Mouse brain cells.
Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064) has 20 independent reviews from customers.
Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064) specifically detects TMEM119 (UniProt ID: Q4V9L6; Molecular weight: 27kDa) and is sold in a convenient trial size to enable initial testing (10 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone 28-3 - Anti-TMEM119 antibody [28-3] - BSA and Azide free ab234501.
TMEM119 is a marker for microglia, the resident immune cells of the brain. In Alzheimer's disease (AD) research, TMEM119 is used to differentiate microglia from other brain-resident cells and infiltrating macrophages. Altered TMEM119 expression in AD brains reflects microglial activation and neuroinflammation, making it a valuable tool for studying the immune response and pathology in Alzheimer's disease.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.
TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.
TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-κB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.
TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab209064 staining TMEM119 in Mouse corpus callosum sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 0.5% BSA for 1 hour at 23°C. Samples were incubated with primary antibody at 1.4μg/ml for 18 hours at 4°C. An Alexa Fluor® 488 -conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
TMEM119 (green), Iba1 (red) and DAPI (blue)
Female C57BL/6 mice (6–8 weeks) were deeply anesthetized with 3% chloral hydrate and a laminectomy was performed. After fixing the spine, 1 μl of 1% lysolecithin in a 0.9% sodium chloride solution was injected into the dorsal funiculus at the level of the T11–T12 vertebrae. The day of lysolecithin injection was designated day 0 (0 dpi). The spinal cord around the injection point was isolated and cut into serial cryosections.
Tissue sections were fixed, permeabilized, and incubated with the primary antibody overnight at 4°C, followed by 2 h of incubation with TRITC‐ or FITC‐conjugated secondary antibodies. Then, the samples were counterstained with Hoechst 33342.
Representative images of sham (d–g) and hypoperfusion (h–k) at 12 weeks post-surgery are shown to illustrate Iba-1 immunostaining in sham (d) and hypoperfused (h); TMEM119 immunostaining in sham (e) and hypoperfused (i) and then Iba-1/TMEM119 co-localisation in sham (f,g) and hypoperfused (j,k) white matter. All Iba-1+ cells in both sham and hypoperfused cohorts were also TMEM119+ indicating that the cells in the corpus callosum were resident microglia. Scale bars; d-f and h-j are 50μm, g and k 10 μm. The number of microglial cells significantly correlated with nodal gap length.
Free floating cryo-preserved sections cut at 30 μm thickness. Sections were incubated with the primary antibodies (anti-Iba-1 (1/100) and anti-TMEM119 (1/500, ab209064)) overnight at 4°C. Sections were stained at the outset with haematoxylin and eosin to determine the presence and absence of ischemic neuronal perikaryal damage as part of the inclusion/exclusion criteria.
IHC image of TMEM119 staining in a section of frozen normal mouse brain wild type (upper panel) and TMEM119 knockout (lower panel). No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in PBS containing 0.5% (v/v) Triton X-100, 0.3 M (w/v) glycine and 1% (w/v) BSA for 1 h at room temperature. The section was then incubated overnight at +4°C in PBS containing 0.5% (v/v) Triton X-100 and 1% (w/v) BSA with ab209064 at 0.5 μg/ml. The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150087 (shown in red) used at 2 μg/ml for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Images were taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
IHC image of TMEM119 and Iba1 co-staining in a section of formalin-fixed paraffin-embedded normal mouse brain. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in PBS containing 0.5% (v/v) Triton X-100, 0.3 M (w/v) glycine and 1% (w/v) BSA for 1 h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.5% (v/v) Triton X-100 and 1% (w/v) BSA with ab209064 at 1 μg/ml and Anti-Iba1 antibody ab5076 at 5 μg/ml. The secondary antibodies were Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150087 (shown in red) and Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed ab150133 (shown in green) used at 2 μg/ml for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Images were taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
IHC image of TMEM119 staining in a section of formalin fixed, paraffin embedded normal mouse brain. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in PBS containing 0.5% (v/v) Triton X-100, 0.3 M (w/v) glycine and 1% (w/v) BSA for 1 h at room temperature. The section was then incubated overnight at +4°C in PBS containing 0.5% (v/v) Triton X-100 and 1% (w/v) BSA with ab209064 at 0.1 μg/ml. The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150087 (shown in red) used at 2 μg/ml for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
IHC image of TMEM119 staining in a section of frozen normal mouse brain. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in PBS containing 0.5% (v/v) Triton X-100, 0.3 M (w/v) glycine and 1% (w/v) BSA for 1 h at room temperature. The section was then incubated overnight at +4°C in PBS containing 0.5% (v/v) Triton X-100 and 1% (w/v) BSA with ab209064 at 0.5 μg/ml. The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150087 (shown in red) used at 2 μg/ml for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
IHC image of TMEM119 staining in a section of formalin fixed, paraffin embedded normal mouse brain, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab209064, 0.5 μg/ml, for 15 mins at room temperature. A goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with ab209064 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.
Normal (WT) mouse brain, stained for TMEM119 (red), Iba1 (green) and DAPI (blue). Samples were baked onto slides for 10 minutes at 60oC, rehydrated with PBS and blocked with blocking buffer (10% serum in PBST). ab209064 at a concentration of 1 μg/mL was incubated with the sample overnight at 4oC. Slides were washed with PBS and a goat anti-rabbit Alexa Fluor 488® was used as the secondary antibody at a concentration of 4 μg/mL.
Normal mouse choroid plexus, stained for TMEM119 (red), Iba1 (green) and DAPI (blue). Choroid plexus macrophages are positive for Iba1 and negative for TMEM119. Samples were baked onto slides for 10 minutes at 60oC, rehydrated with PBS and blocked with blocking buffer (10% serum in PBST). ab209064 at a concentration of 1 μg/mL was incubated with the sample overnight at 4oC. Slides were washed with PBS and a goat anti-rabbit Alexa Fluor 488® was used as the secondary antibody at a concentration of 4 μg/mL.
Immunohistochemical analysis of formalin fixed paraffin embedded mouse cerebrum labelling TMEM119 with ab209064 at 1/1000 dilution. The secondary used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Sections were counterstained with DAPI. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Positive staining on microglial cells of mouse cerebrum. The section was incubated with ab209064 at 4°C overnight.
Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labelling TMEM119 with ab209064 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a ChromoMap DAB kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
ab209064 anti-TMEM119 antibody [28-3] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
IHC image of TMEM119 staining in a section of Mouse cerebrum using ab209064 at 1:50 dilution.The section was fixed with 4% PFA then permeabilized with 0.2% Triton X-100. The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at 1:1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control: PBS instead of the primary antibody.
Positive staining on mouse cerebrum.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com