Products
Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
Highly sensitive kit offering the most promising biomarkers for Alzheimer’s disease diagnostics. Learn about all product ranges with our product overviews.
Resources
Featured events
Make new connections at our global events.
Support
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Rabbit Recombinant Monoclonal TMEM119 antibody. Microglia marker. Suitable for IHC-FoFr, IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 139 publications.
Alternative names=Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-FoFr | IHC-P | IHC-Fr | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended |
Images
IHC-FoFr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5-1 µg/mL | Notes Perform heat mediated antigen retrieval with citrate buffer pH 6. Incubate the section with primary antibody at 4 ℃ overnight. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.1-0.5 µg/mL | Notes Tris-EDTA buffer preferred. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Tris-EDTA buffer preferred. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Tris-EDTA buffer preferred. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5-1 µg/mL | Notes We recommend using 0.3-0.5% Triton X-100. Perform heat mediated antigen retrieval before IHC-Fr staining protocol, if the signal is too weak. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes We recommend using 0.3-0.5% Triton X-100. Perform heat mediated antigen retrieval before IHC-Fr staining protocol, if the signal is too weak. |
Species Human | Dilution info - | Notes We recommend using 0.3-0.5% Triton X-100. Perform heat mediated antigen retrieval before IHC-Fr staining protocol, if the signal is too weak. |
Target data
Function
Plays an important role in bone formation and normal bone mineralization (PubMed:26207632, PubMed:22416756, PubMed:20025746). Promotes the differentiation of myoblasts into osteoblasts (PubMed:22416756, PubMed:20025746, PubMed:22579779). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway (PubMed:21239498, PubMed:22579779). Upregulates the expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Essential for normal spermatogenesis and late testicular differentiation (PubMed:26207632).
Recommended products
Rabbit Recombinant Monoclonal TMEM119 antibody. Microglia marker. Suitable for IHC-FoFr, IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 139 publications.
Alternative names=Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
28-3
- Purification technique
Affinity purification Protein A
- Specificity
This antibody recognizes mouse Tmem119, a transmembrane protein that has been reported to be a highly specific microglia marker that is not expressed by macrophages or other immune or neural cell types (Bennett et al., 2016).
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
This Tmem119 antibody has been knockout validated in IHC, meaning it demonstrated the expected staining in wild type mouse brain sections and no staining was observed in Tmem119 knockout mouse brain sections. This data is shown on this datasheet in the images section. To detect mouse Tmem119 by flow cytometry, we recommend using ab210405. To detect human TMEM119 by IHC, we recommend using ab185333.
The 28-3 clone to mouse Tmem119 is exclusively manufactured and sold by Abcam.
IHC-Frozen protocol advice:
For immunohistochemistry on frozen sections, it is recommended that a high concentration of Triton X-100 (0.5%) is used during permeabilization and antibody incubation steps. This may increase the proportion of microglia that stain positive for Tmem119.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com