AB185333

Anti-TMEM119 antibody - C-terminal - Microglial marker

Name: Anti-TMEM119 antibody - C-terminal (ab185333) | Abcam
Brand: Abcam Limited
SKU: AB185333
Price: 545 USD
Availability: InStock
Rating: 3 (7 reviews)

(7 Reviews)

(58 Publications)

Anti-TMEM119 antibody - C-terminal (ab185333) is a rabbit polyclonal antibody detecting TMEM119 in IHC-P. Suitable for Human.

- Over 40 publications

View Alternative Names

PSEC0199, UNQ731/PRO1415, TMEM119, Transmembrane protein 119, Osteoblast induction factor, OBIF

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal - Microglial marker (AB185333)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal - Microglial marker (AB185333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling TMEM119 with ab185333 at a dilution of 1/1000.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal - Microglial marker (AB185333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling TMEM119 with ab185333 at a dilution of 1/1000.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal - Microglial marker (AB185333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling TMEM119 with ab185333 at a dilution of 1/1000.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal - Microglial marker (AB185333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling TMEM119 with ab185333 at a dilution of 1/1000.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TMEM119 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q4V9L6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Product details

Product Specifications
Anti-TMEM119 antibody - C-terminal (ab185333) is a rabbit polyclonal antibody and is validated for use in IHC-P in human samples.
Anti-TMEM119 antibody - C-terminal (ab185333) specifically detects TMEM119 (UniProt ID: Q4V9L6; Molecular weight: 26kDa) and is sold in 100µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-TMEM119 antibody - C-terminal (ab185333) has high sensitivity and specificity.
Anti-TMEM119 antibody - C-terminal (ab185333) has been cited over 40 times in peer reviewed journals and is trusted by the scientific community.
Anti-TMEM119 antibody - C-terminal (ab185333) has 7 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.

Biological function summary

TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.

Pathways

TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.

TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts (PubMed : 20025746). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Up-regulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation (By similarity).

See full target information TMEM119

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Publications (58)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 19:1557645 PubMed40842561

2025

Decreased neuronal and increased endothelial fractalkine expression are associated with neuroinflammation in Parkinson's disease and related disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping Chu,Ashley S Harms,Ashley Boehringer,Jeffrey H Kordower

Molecular psychiatry 30:4776-4791 PubMed40527900

2025

Cell autonomous microglia defects in a stem cell model of frontotemporal dementia tau.

Applications

Unspecified application

Species

Unspecified reactive species

Abhirami K Iyer,Lisa Vermunt,Farzaneh S Mirfakhar,Miguel Minaya,Mariana Acquarone,Rama Krishna Koppisetti,Arun Renganathan,Shih-Feng You,Emma P Danhash,Kylie J Schache,Anthony Verbeck,Grant Galasso,Scott M Lee,Guangming Huang,Katherine J Miller,Jacob Marsh,Alissa L Nana,Salvatore Spina,William W Seeley,Lea T Grinberg,Sally Temple,Charlotte E Teunissen,Chihiro Sato,Celeste M Karch

Nature communications 16:1577 PubMed39948387

2025

Unraveling microglial spatial organization in the developing human brain with DeepCellMap, a deep learning approach coupled with spatial statistics.

Applications

Unspecified application

Species

Unspecified reactive species

Theo Perochon,Zeljka Krsnik,Marco Massimo,Yana Ruchiy,Alejandro Lastra Romero,Elyas Mohammadi,Xiaofei Li,Katherine R Long,Laura Parkkinen,Klas Blomgren,Thibault Lagache,David A Menassa,David Holcman

Molecular neurodegeneration 19:90 PubMed39593143

2024

Human VCP mutant ALS/FTD microglia display immune and lysosomal phenotypes independently of GPNMB.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin E Clarke,Oliver J Ziff,Giulia Tyzack,Marija Petrić Howe,Yiran Wang,Pierre Klein,Claudia A Smith,Cameron A Hall,Adel Helmy,Michael Howell,Gavin Kelly,Rickie Patani

Acta neuropathologica 147:92 PubMed38801558

2024

Neuropathological findings in Down syndrome, Alzheimer's disease and control patients with and without SARS-COV-2: preliminary findings.

Applications

Unspecified application

Species

Unspecified reactive species

Ann-Charlotte E Granholm,Elisabet Englund,Anah Gilmore,Elizabeth Head,William H Yong,Sylvia E Perez,Samuel J Guzman,Eric D Hamlett,Elliott J Mufson

Science advances 10:eadj3301 PubMed38758780

2024

Hypoxia coordinates the spatial landscape of myeloid cells within glioblastoma to affect survival.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Haley,Leoma Bere,James Minshull,Sokratia Georgaka,Natalia Garcia-Martin,Gareth Howell,David J Coope,Federico Roncaroli,Andrew King,David C Wedge,Stuart M Allan,Omar N Pathmanaban,David Brough,Kevin N Couper

Journal of neurovirology 30:362-379 PubMed38600307

2024

Modeling HIV-1 infection and NeuroHIV in hiPSCs-derived cerebral organoid cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Donadoni,Senem Cakir,Anna Bellizzi,Michael Swingler,Ilker K Sariyer

Journal of neuroinflammation 21:50 PubMed38365833

2024

Advanced patient-specific microglia cell models for pre-clinical studies in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Cuní-López,Romal Stewart,Lotta E Oikari,Tam Hong Nguyen,Tara L Roberts,Yifan Sun,Christine C Guo,Michelle K Lupton,Anthony R White,Hazel Quek

Cell research 34:193-213 PubMed38177242

2024

Decoding the spatiotemporal regulation of transcription factors during human spinal cord development.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchao Shi,Luwei Huang,Hao Dong,Meng Yang,Wenyu Ding,Xiang Zhou,Tian Lu,Zeyuan Liu,Xin Zhou,Mengdi Wang,Bo Zeng,Yinuo Sun,Suijuan Zhong,Bosong Wang,Wei Wang,Chonghai Yin,Xiaoqun Wang,Qian Wu

NPJ Parkinson's disease 10:1 PubMed38167744

2024

Aggregate-prone brain regions in Parkinson's disease are rich in unique N-terminus α-synuclein conformers with high proteolysis susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

James A Wiseman,Helen C Murray,Richard L M F Faull,Michael Dragunow,Clinton P Turner,Birger Victor Dieriks,Maurice A Curtis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TMEM119 antibody - C-terminal - Microglial marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal - Microglial marker (AB185333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal - Microglial marker (AB185333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal - Microglial marker (AB185333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - C-terminal - Microglial marker (AB185333)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (58)

Product promise