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AB306584

Anti-TMEM119 antibody [EPR25865-89] - BSA and Azide free

  • BOND RX™ Validated
  • Recombinant
  • Advanced Validation
  • RabMAb
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Rabbit Recombinant Monoclonal TMEM119 antibody. Carrier free. Suitable for mIHC, IHC-P and reacts with Human, Transfected cell lysate - Human samples.

View Alternative Names

PSEC0199, UNQ731/PRO1415, TMEM119, Transmembrane protein 119, Osteoblast induction factor, OBIF

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - BSA and Azide free (AB306584)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - BSA and Azide free (AB306584)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - BSA and Azide free (AB306584)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - BSA and Azide free (AB306584)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - BSA and Azide free (AB306584)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - BSA and Azide free (AB306584)
Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] - BSA and Azide free (AB306584)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] - BSA and Azide free (AB306584)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-TMEM119 (green; Opal™520) and anti-GFAP (red; Opal™570) on human cerebrum.

Panel B : anti-GPR17 staining oligodendrocytes in human cerebrum.

Panel C : anti-TMEM119 staining microglia in human cerebrum.

Panel D : anti-GFAP staining astrocytes in human cerebrum.

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab306583 at a 1/2000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab306583, the same antibody clone in a different buffer formulation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25865-89

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, mIHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.
Biological function summary

TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.

Pathways

TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.

TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts (PubMed : 20025746). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Up-regulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation (By similarity).
See full target information TMEM119
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Product promise

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For full details, please see our Terms & Conditions

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