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Rabbit Recombinant Monoclonal TMEM119 antibody. Suitable for mIHC, IHC-P and reacts with Human, Transfected cell lysate - Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (AB306583), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (AB306583), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (AB306583), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] (AB306583), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] (AB306583), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-PWBIHC-FrICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Transfected cell lysate - Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts (PubMed:20025746). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Up-regulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation (By similarity).

Alternative names

PSEC0199, UNQ731/PRO1415, TMEM119, Transmembrane protein 119, Osteoblast induction factor, OBIF

Recommended products

Rabbit Recombinant Monoclonal TMEM119 antibody. Suitable for mIHC, IHC-P and reacts with Human, Transfected cell lysate - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25865-89
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.

Biological function summary

TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.

Pathways

TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.

Associated diseases and disorders

TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (ab306583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (ab306583)

    TMEM119 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-TMEM119 antibody

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling TMEM119 with ab306583 at 1/2000 (0.255 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on microglial cells of human cerebrum. The section was incubated with ab306583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (ab306583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (ab306583)

    TMEM119 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human tonsil using rabbit Anti-TMEM119 antibody

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TMEM119 with ab306583 at 1/2000 (0.255 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human tonsil. The section was incubated with ab306583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (ab306583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (ab306583)

    TMEM119 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human endometrial carcinoma using rabbit Anti-TMEM119 antibody

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling TMEM119 with ab306583 at 1/2000 (0.255 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on stromal cells of human endometrial carcinoma. The section was incubated with ab306583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] (ab306583), expandable thumbnail

    Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] (ab306583)

    Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue sections.

    Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-TMEM119 (green; Opal™520) and anti-GFAP (red; Opal™570) on human cerebrum.

    Panel B: anti-GPR17 staining oligodendrocytes in human cerebrum.

    Panel C: anti-TMEM119 staining microglia in human cerebrum.

    Panel D: anti-GFAP staining astrocytes in human cerebrum.

    The section was incubated in three rounds of staining: in the order of Anti-GPCR GPR17 antibody [EPR26423-34] ab316105 at a 1/500 dilution, ab306583 at a 1/2000 dilution, and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] (ab306583), expandable thumbnail

    Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] (ab306583)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human astrocytoma tissue staining TREM2 with Anti-TREM2 antibody [EPR26209-22] ab318262 at a 1:100 (5.29 ug/ml) dilution, ab306583 anti-TMEM119 used at 1:2000 (0.255 ug/ml) dilution and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 anti-GFAP used at a 1:1000 (1.325 ug/ml) dilution.

    Panel A: merged staining of anti-TREM2 (green; Opal™520), anti-TMEM119 (magenta; Opal™690) and anti-GFAP (yellow; Opal™570) on human astrocytoma.

    Panel B: anti-TREM2 staining microglia in human astrocytoma.

    Panel C: anti-TMEM119 staining microglia in human astrocytoma.

    Panel D: anti-GFAP staining astrocyte in human astrocytoma.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-TREM2 antibody [EPR26209-22] ab318262, ab306583 and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] (ab306583), expandable thumbnail

    Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] (ab306583)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue staining TREM2 with Anti-TREM2 antibody [EPR26209-22] ab318262 at a 1:100 (5.29 ug/ml) dilution, ab306583 anti-TMEM119 used at 1:2000 (0.255 ug/ml) dilution and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 anti-GFAP used at a 1:1000 (1.325 ug/ml) dilution.

    Panel A: merged staining of anti-TREM2 (green; Opal™520), anti-TMEM119 (magenta; Opal™690) and anti-GFAP (yellow; Opal™570) on human cerebrum.

    Panel B: anti-TREM2 staining microglia in human cerebrum.

    Panel C: anti-TMEM119 staining microglia in human cerebrum.

    Panel D: anti-GFAP staining astrocytes in human cerebrum.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-TREM2 antibody [EPR26209-22] ab318262, ab306583 and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

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