Anti-TMEM119 antibody [EPR25865-89] - Microglial marker

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (AB306583)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling TMEM119 with ab306583 at 1/2000 (0.255 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on stromal cells of human endometrial carcinoma. The section was incubated with ab306583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (AB306583)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling TMEM119 with ab306583 at 1/2000 (0.255 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on microglial cells of human cerebrum. The section was incubated with ab306583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (AB306583)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TMEM119 with ab306583 at 1/2000 (0.255 µg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human tonsil. The section was incubated with ab306583 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (AB306583)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue sections.

Panel A : merged staining of anti-GPR17 (magenta; Opal™690), anti-TMEM119 (green; Opal™520) and anti-GFAP (red; Opal™570) on human cerebrum.

Panel B : anti-GPR17 staining oligodendrocytes in human cerebrum.

Panel C : anti-TMEM119 staining microglia in human cerebrum.

Panel D : anti-GFAP staining astrocytes in human cerebrum.

The section was incubated in three rounds of staining : in the order of ab316105 at a 1/500 dilution, ab306583 at a 1/2000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (AB306583)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue staining TREM2 with ab318262 at a 1 : 100 (5.29 ug/ml) dilution, ab306583 anti-TMEM119 used at 1 : 2000 (0.255 ug/ml) dilution and ab218309 anti-GFAP used at a 1 : 1000 (1.325 ug/ml) dilution.

Panel A : merged staining of anti-TREM2 (green; Opal™520), anti-TMEM119 (magenta; Opal™690) and anti-GFAP (yellow; Opal™570) on human cerebrum.
Panel B : anti-TREM2 staining microglia in human cerebrum.
Panel C : anti-TMEM119 staining microglia in human cerebrum.
Panel D : anti-GFAP staining astrocytes in human cerebrum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab318262, ab306583 and ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (AB306583)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human astrocytoma tissue staining TREM2 with ab318262 at a 1 : 100 (5.29 ug/ml) dilution, ab306583 anti-TMEM119 used at 1 : 2000 (0.255 ug/ml) dilution and ab218309 anti-GFAP used at a 1 : 1000 (1.325 ug/ml) dilution.

Panel A : merged staining of anti-TREM2 (green; Opal™520), anti-TMEM119 (magenta; Opal™690) and anti-GFAP (yellow; Opal™570) on human astrocytoma.
Panel B : anti-TREM2 staining microglia in human astrocytoma.
Panel C : anti-TMEM119 staining microglia in human astrocytoma.
Panel D : anti-GFAP staining astrocyte in human astrocytoma.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab318262, ab306583 and ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.