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AB317220

Anti-TMEM119 antibody [HL2415]

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Rabbit Monoclonal TMEM119 antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tmem119.

View Alternative Names

Transmembrane protein 119, Osteoblast induction factor, OBIF, Tmem119

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [HL2415] (AB317220)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [HL2415] (AB317220)

ab317220 detects TMEM119 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. TMEM119 stained by ab317220 diluted at 1 : 50. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [HL2415] (AB317220)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [HL2415] (AB317220)

ab317220 detects TMEM119 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. Green : TMEM119 stained by ab317220 diluted at 1 : 100. Red : beta Tubulin 3/ Tuj1 a Cytoskeleton marker stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1 : 500. Blue : Fluoroshield with DAPI. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [HL2415] (AB317220)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [HL2415] (AB317220)

ab317220 detects TMEM119 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat brain. TMEM119 stained by ab317220 diluted at 1 : 50. Red : beta Tubulin 3/ Tuj1 a Cytoskeleton marker stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1 : 500. Blue : Fluoroshield with DAPI. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2415

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Tmem119.

Q8R138

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.
Biological function summary

TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.

Pathways

TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.

TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in bone formation and normal bone mineralization (PubMed : 20025746, PubMed : 22416756, PubMed : 26207632). Promotes the differentiation of myoblasts into osteoblasts (PubMed : 20025746, PubMed : 22416756, PubMed : 22579779). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway (PubMed : 21239498, PubMed : 22579779). Up-regulates the expression of ATF4 which plays a central role in osteoblast differentiation (PubMed : 24362451). Essential for normal spermatogenesis and late testicular differentiation (PubMed : 26207632).
See full target information Tmem119
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Product promise

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