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AB325668

Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric)

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Chicken Monoclonal tmem119 antibody. Suitable for IHC-P, WB and reacts with Rat, Transfected cell lysate samples.
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)

Representative section of formalin fixed, paraffin-embedded rat brain stem showing microglia cells stained by ab325668 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. Magnification 100X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)

Representative section of formalin fixed, paraffin-embedded rat brain stem showing microglia cells stained by ab325668 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. Magnification 400X.

Western blot - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)
  • WB

Supplier Data

Western blot - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)

Western blotting of mock transfected and TMEM119-FLAG transfected COS-7 lysates with ab325668 (10 µg/mL) and detected with anti-chicken-HRP. The antibody recognizes exogenous TMEM119 only in TMEM119-FLAG transfected COS-7 lysates at 40 kDa.

All lanes:

Western blot - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (ab325668) at 10 µg/mL

Lane 1:

mock transfected COS-7 lysates

Lane 2:

TMEM119-FLAG transfected COS-7 lysates

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 40 kDa

false

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

L128/43

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

No cross-reactivity reported.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L128/43 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L128/43 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information tmem119
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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