AB185337

Anti-TMEM119 antibody - Microglial marker

Name: Anti-TMEM119 antibody - Microglial marker (ab185337) | Abcam
Brand: Abcam Limited
SKU: AB185337
Price: 400 USD
Availability: InStock
Rating: 2 (13 reviews)

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What is this?

(13 Reviews)

(12 Publications)

Rabbit Polyclonal TMEM119 antibody. Microglia marker. Suitable for IHC-P and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TMEM119 aa 100-200.

View Alternative Names

PSEC0199, UNQ731/PRO1415, TMEM119, Transmembrane protein 119, Osteoblast induction factor, OBIF

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

Immunohistochemistry analysis of Human tonsil tissue labeling TMEM119 with ab185337 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

Paraffin-embedded human testis tissue stained for TMEM119 using ab185337 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

Paraffin-embedded human liver tissue stained for TMEM119 using ab185337 at 1/50 dilution in immunohistochemical analysis.

Negative control.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

Paraffin-embedded human cerebral cortex tissue stained for TMEM119 using ab185337 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

Paraffin-embedded human lymph node tissue stained for TMEM119 using ab185337 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TMEM119 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q4V9L6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM119 also known as transmembrane protein 119 plays a role in cell membrane dynamics. It has a molecular mass of approximately 35 kDa. TMEM119 is specifically expressed in microglia in the central nervous system. Microglia serve essential functions in brain homeostasis and immune response. Given its expression profile TMEM119 serves as a marker to identify microglia especially in research focused on neurological tissues. Researchers often utilize techniques like microglial flow cytometry and microglial staining for identification and quantification of microglial populations often in frozen marker studies.

Biological function summary

TMEM119 regulates signaling pathways that maintain microglial identity and function but does not typically form part of larger complexes. It modulates microglial activation states and influences responses to central nervous system injury and inflammation. As a membrane protein it may interact with other surface molecules although specific binding partners are not well-characterized in current literature. Researchers value TMEM119 as a reliable marker in studies aiming to distinguish brain-resident microglia from infiltrating peripheral macrophages.

Pathways

TMEM119 functions as a modulator within inflammatory and neuroimmune pathways. It plays roles in the NF-kB signaling and the neuroinflammatory cascade. Its relationship with proteins like CD68 and Iba1 indicates it may influence pathways tied to immune responses and phagocytosis within nervous system contexts. Understanding the interactions and co-expression of TMEM119 with these proteins helps clarify its contribution to neuroimmune functions.

TMEM119 links with neurological conditions such as Alzheimer's disease and multiple sclerosis. Altered expression of TMEM119 appears in disease states potentially contributing to pathogenesis through disrupted inflammatory responses. Its association with proteins such as TREM2 in Alzheimer's suggests that TMEM119 may play indirect roles in the progression of neurodegeneration. Furthermore high TMEM119 levels in pathological samples provide insights into microglial involvement in disease progression and potential targets for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts (PubMed : 20025746). May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Up-regulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation (By similarity).

See full target information TMEM119

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:47 PubMed38347588

2024

Progranulin haploinsufficiency mediates cytoplasmic TDP-43 aggregation with lysosomal abnormalities in human microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Wonjae Sung,Min-Young Noh,Minyeop Nahm,Yong Sung Kim,Chang-Seok Ki,Young-Eun Kim,Hee-Jin Kim,Seung Hyun Kim

Biological psychiatry 95:676-686 PubMed37573007

2023

Loss of Function in the Neurodevelopmental Disease and Schizophrenia-Associated Gene CYFIP1 in Human Microglia-like Cells Supports a Functional Role in Synaptic Engulfment.

Applications

Unspecified application

Species

Unspecified reactive species

Steven D Sheridan,Joy E Horng,Hana Yeh,Liam McCrea,Jennifer Wang,Ting Fu,Roy H Perlis

Methods in molecular biology (Clifton, N.J.) 2683:53-68 PubMed37300766

2023

Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Wei Chen,Yu-Hui Wong

Scientific reports 13:7907 PubMed37193782

2023

Cannabidiol inhibits neuroinflammatory responses and circuit-associated synaptic loss following damage to a songbird vocal pre-motor cortical-like region.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Tripson,Karen Litwa,Ken Soderstrom

Frontiers in molecular neuroscience 16:1128545 PubMed37251648

2023

Effects of a neurokinin-1 receptor antagonist in the acute phase after thoracic spinal cord injury in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Guoli Zheng,Anna-Kathrin Harms,Mohamed Tail,Hao Zhang,Alan Nimmo,Thomas Skutella,Karl Kiening,Andreas Unterberg,Klaus Zweckberger,Alexander Younsi

Journal of neuroscience research 101:3-19 PubMed36200530

2022

Development of a novel bioengineered 3D brain-like tissue for studying primary blast-induced traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Dustin M Snapper,Bianca Reginauld,Volha Liaudanskaya,Vincent Fitzpatrick,Yeonho Kim,Irene Georgakoudi,David L Kaplan,Aviva J Symes

International forum of allergy & rhinology 13:242-254 PubMed35984636

2022

Eosinophil-derived interferon-γ drives transmembrane protein 119-induced new bone formation in chronic rhinosinusitis with nasal polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Sungsin Jo,Bong Joon Jin,Seung Hoon Lee,Hye-Ryeong Jo,Joo Mi Park,Kyung-Gyun Hwang,Mina Rho,Tae-Hwan Kim,Seok Hyun Cho

Scientific reports 12:12606 PubMed35871163

2022

Human iPSC co-culture model to investigate the interaction between microglia and motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Björn F Vahsen,Elizabeth Gray,Ana Candalija,Kaitlyn M L Cramb,Jakub Scaber,Ruxandra Dafinca,Antigoni Katsikoudi,Yinyan Xu,Lucy Farrimond,Richard Wade-Martins,William S James,Martin R Turner,Sally A Cowley,Kevin Talbot

Brain pathology (Zurich, Switzerland) 32:e13101 PubMed35748290

2022

Differential perivascular microglial activation in the deep white matter in vascular dementia developed post-stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiki Hase,Kamar E Ameen-Ali,Rachel Waller,Julie E Simpson,Charlotte Stafford,Ayushi Mahesh,Lucy Ryan,Lucy Pickering,Caroline Bodman,Mai Hase,Delphine Boche,Karen Horsburgh,Stephen B Wharton,Raj N Kalaria

Frontiers in neurology 10:728 PubMed31354608

2019

Human Inner Ear Immune Activity: A Super-Resolution Immunohistochemistry Study.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Charlotta Kämpfe Nordström,Niklas Danckwardt-Lillieström,Helge Rask-Andersen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TMEM119 antibody - Microglial marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody - Microglial marker (AB185337)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise