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AB222237

Anti-TMEM135 antibody

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(1 Publication)

Rabbit Polyclonal TMEM135 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TMEM135 aa 150-250.

View Alternative Names

Transmembrane protein 135, Peroxisomal membrane protein 52, PMP52, TMEM135

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TMEM135 antibody (AB222237)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMEM135 antibody (AB222237)

4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for TMEM135 (green) using ab222237 at 4 μg/ml in ICC/IF.

Western blot - Anti-TMEM135 antibody (AB222237)
  • WB

Supplier Data

Western blot - Anti-TMEM135 antibody (AB222237)

All lanes:

Western blot - Anti-TMEM135 antibody (ab222237) at 1/250 dilution

All lanes:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Predicted band size: 52 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TMEM135 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q86UB9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM135 also known as Transmembrane Protein 135 has a molecular mass of approximately 50 kDa. This protein is expressed in multiple tissues with high levels found in adipose tissue and heart. It functions mechanically by regulating lipid metabolism and its activity is associated with mitochondria. TMEM135 helps maintain mitochondrial dynamics including fusion and fission processes which are critical for proper energy homeostasis in cells.
Biological function summary

TMEM135 participates in energy balance and lipid metabolism. This protein does not function alone; it is part of complexes involving mitochondrial membranes. Within these complexes TMEM135 helps orchestrate the metabolism of lipids impacting overall energy distribution in cells. Its role extends to regulating oxidative stress suggesting it plays a part in cellular responses to metabolic changes.

Pathways

TMEM135 functions contribute to the mitochondrial apoptosis pathway and the peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways are essential in controlling cell viability and energy metabolism respectively. TMEM135 interacts with proteins like DRP1 and PGC-1α which further help in mitochondrial division and energy metabolism regulation reflecting its importance in cellular energetics.

TMEM135 displays implications in metabolic syndrome and cardiovascular disease. Its influence on lipid metabolism and mitochondrial function links it to these conditions. In metabolic syndrome altered function of TMEM135 may affect PGC-1α involved in the regulation of glucose and lipid homeostasis. In cardiovascular disease disrupted mitochondrial dynamics due to TMEM135 dysfunction can lead DRP1-driven imbalances resulting in compromised heart function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in mitochondrial metabolism by regulating the balance between mitochondrial fusion and fission. May act as a regulator of mitochondrial fission that promotes DNM1L-dependent fission through activation of DNM1L. May be involved in peroxisome organization.
See full target information TMEM135
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 44:1627-1637 PubMed32945512

2020

MicroRNA‑137 exerts protective effects on hypoxia‑induced cell injury by inhibiting autophagy/mitophagy and maintaining mitochondrial function in breast cancer stem‑like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiongying Hu,Yun Yuan,Yeke Wu,Yongliang Huang,Ziyi Zhao,Chong Xiao
View all publications
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Product promise

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