AB97624

Anti-TMEM147 antibody

Name: Anti-TMEM147 antibody (ab97624) | Abcam
Brand: Abcam Limited
SKU: AB97624

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(4 Publications )

Rabbit Polyclonal TMEM147 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TMEM147 aa 50-150.

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-TMEM147 antibody (AB97624)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TMEM147 antibody (AB97624)

1/200 ab97624 staining TMEM147 in paraformaldehyde-fixed A431 cells by immunofluorescence.
The lower image was co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM147 antibody (AB97624)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM147 antibody (AB97624)

1/500 ab97624 staining TMEM147 in paraffin-embedded NCI-N87 xenograft by immunohistochemistry.

Unknown

Western blot - Anti-TMEM147 antibody (AB97624)

12% SDS PAGE

All lanes:

Western blot - Anti-TMEM147 antibody (ab97624) at 1/1500 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TMEM147 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q9BVK8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM147 also known as NBR1 is a transmembrane protein with a molecular mass of approximately 28 kDa. It resides primarily in the endoplasmic reticulum and is widely expressed across various tissues such as the brain and liver. The protein spans the membrane multiple times indicating its role in maintaining cellular structure and function within the membrane environment. TMEM147 is often associated with regulatory processes that affect membrane-related activities.

Biological function summary

TMEM147 interacts with proteins in the ribosome maturation process and is involved in assembling the nuclear pore complex. It forms part of the N-oligosaccharyltransferase (OST) complex which plays an essential role in protein modification through glycosylation. This complex activity helps in the proper folding and stability of proteins necessary for cell function. Therefore TMEM147 influences cellular processes by regulating the maturation and transport of proteins within cells.

Pathways

TMEM147 contributes significantly to the N-glycosylation and protein biosynthesis pathways. Within these pathways the protein interacts with enzymes like STT3A and STT3B which are key catalytic components of the oligosaccharyltransferase complex. Through this interaction TMEM147 ensures proper protein folding and efficiency in glycoprotein processing. It also influences cellular homeostasis and stress responses by coordinating with proteins related to ER stress pathways.

TMEM147 shows a connection to neurodegenerative diseases and certain types of cancer. Studies highlight its involvement in Alzheimer's disease where it interacts with the γ-secretase complex including proteins like presenilin-1 (PSEN1) affecting amyloid-beta precursor protein processing. In cancer abnormal TMEM147 expression correlates with altered N-glycosylation patterns leading to changes in tumor cell behavior. This association suggests TMEM147's potential role as a biomarker or therapeutic target in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed : 32820719, PubMed : 36261522). The MPT complex takes over after the SEC61 complex : following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions (PubMed : 36261522). Also acts as a negative regulator of CHRM3 function, most likely by interfering with its trafficking to the cell membrane (PubMed : 21056967). Negatively regulates CHRM3-mediated calcium mobilization and activation of RPS6KA1/p90RSK activity (PubMed : 21056967). Regulates LBR localization to the nucleus inner membrane (PubMed : 32694168).

See full target information TMEM147

Alternative Names

BOS complex subunit TMEM147, Protein NIFIE 14, Transmembrane protein 147, TMEM147

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Publications (4)

Recent publications for all applications. Explore the full listand refine your search

Journal of translational medicine 22:1082 PubMed39614244

2024

Innovations in radiotherapy for tongue squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Songling Hu,Xiaofei Li,Bin Yang,Tian Yu,Fangyu Yi,Xiurong Qin,Cong Chen,Can Wang,Xin Yu,Jing Zhu

Journal of experimental & clinical cancer research : CR 42:286 PubMed37891677

2023

TMEM147 aggravates the progression of HCC by modulating cholesterol homeostasis, suppressing ferroptosis, and promoting the M2 polarization of tumor-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Huang,Huayang Pan,Jing Sun,Jiaming Wu,Qiyue Xuan,Jinge Wang,Shanjia Ke,Shounan Lu,Zihao Li,Zhigang Feng,Yongliang Hua,Qingan Yu,Bing Yin,Baolin Qian,Menghua Zhou,Yanan Xu,Miaoyu Bai,Yingmei Zhang,Yaohua Wu,Yong Ma,Hongchi Jiang,Wenjie Dai

Technology in cancer research & treatment 22:15330338231178391 PubMed37489256

2023

Tubulin Alpha-1b as a Potential Biomarker for Lung Adenocarcinoma Diagnosis and Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Li Pang,Hong-Fei Du,Fang Nie,Xiang-Gui Yang,Ying Xu

Analytical cellular pathology (Amsterdam) 2023:4413049 PubMed37305689

2023

TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Cheng,Jutang Li,Ming Xu,Qun Bao,Jiaoxiang Wu,Peng Sun,Bo Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TMEM147 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TMEM147 antibody (AB97624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM147 antibody (AB97624)

Western blot - Anti-TMEM147 antibody (AB97624)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (4)

Product promise