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Rabbit Recombinant Monoclonal TMEM161A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (AB250284), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (AB250284), expandable thumbnail
  • Western blot - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (AB250284), expandable thumbnail
  • Western blot - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (AB250284), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

May play a role in protection against oxidative stress. Overexpression leads to reduced levels of oxidant-induced DNA damage and apoptosis.

Alternative names

UNQ582/PRO1152, TMEM161A, Transmembrane protein 161A, Adaptive response to oxidative stress protein 29, AROS-29

Recommended products

Rabbit Recombinant Monoclonal TMEM161A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14369
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250284 is the carrier-free version of Anti-TMEM161A antibody [EPR14369] ab180954.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TMEM161A also known as Transmembrane Protein 161A is a member of the larger transmembrane protein family. Structurally TMEM161A is characterized by multiple transmembrane domains. Its mass is approximately 55 kDa. Researchers have observed its expression in various tissues including the brain and kidney. The exact mechanical role is not fully elucidated but it is suggested to play a role in cellular communication through its membrane localization.

Biological function summary

TMEM161A contributes to cellular processes involving intercellular signaling and ion transport. Although scientists have not yet identified a specific complex that includes TMEM161A its function as a transmembrane protein implies potential involvement in forming channels or receptors. These elements are important for maintaining ionic balances and signaling pathways inside cells indicating TMEM161A as a supportive component in these processes.

Pathways

Recent findings indicate TMEM161A's potential involvement in cell signaling and transport pathways. An important pathway that features TMEM161A activity includes signal transduction where it may interact with proteins like ion channels such as voltage-gated channels. These interactions suggest TMEM161A could modulate responses to cellular environment changes linking it to broader cellular response mechanisms.

Associated diseases and disorders

Scientists have noted potential links between TMEM161A and neurological disorders. Some studies suggest its involvement in conditions like epilepsy where abnormal ion transport plays a significant role. Alterations in TMEM161A expression might impact channel proteins such as voltage gated potassium channels which are important for maintaining action potentials in neurons potentially exacerbating these conditions. Research continues to explore TMEM161A's full role in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)

    This data was developed using Anti-TMEM161A antibody [EPR14369] ab180954, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling TMEM161A with Anti-TMEM161A antibody [EPR14369] ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)

    This data was developed using Anti-TMEM161A antibody [EPR14369] ab180954, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling TMEM161A with Anti-TMEM161A antibody [EPR14369] ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284), expandable thumbnail

    Western blot - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)

    This data was developed using Anti-TMEM161A antibody [EPR14369] ab180954, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-TMEM161A antibody [EPR14369] (Anti-TMEM161A antibody [EPR14369] ab180954) at 1/20000 dilution

    All lanes: Human fetal liver tissue lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa

    Observed band size: 54 kDa

  • Western blot - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284), expandable thumbnail

    Western blot - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)

    This data was developed using Anti-TMEM161A antibody [EPR14369] ab180954, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-TMEM161A antibody [EPR14369] (Anti-TMEM161A antibody [EPR14369] ab180954) at 1/100000 dilution

    Lane 1: HT1080 cell lysate at 20 µg

    Lane 2: HepG2 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 51 kDa, 53 kDa

    Observed band size: 54 kDa, 66 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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