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AB216043

Anti-TMEM166 antibody

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(2 Publications)

Rabbit Polyclonal TMEM166 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EVA1A.

View Alternative Names

FAM176A, TMEM166, SP24, EVA1A, Protein eva-1 homolog A, Protein FAM176A, Transmembrane protein 166

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM166 antibody (AB216043)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM166 antibody (AB216043)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling TMEM166 with ab216043 at 1/30 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM166 antibody (AB216043)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM166 antibody (AB216043)

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling TMEM166 with ab216043 at 1/30 dilution.

Western blot - Anti-TMEM166 antibody (AB216043)
  • WB

Supplier Data

Western blot - Anti-TMEM166 antibody (AB216043)

Gel : 12%SDS-PAGE.

All lanes:

Western blot - Anti-TMEM166 antibody (ab216043) at 1/550 dilution

All lanes:

Human liver lysate at 40 µg

Predicted band size: 17 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human EVA1A. The exact immunogen used to generate this antibody is proprietary information.

Q9H8M9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM166 also known as TN601 is a transmembrane protein involved in various cellular processes. It has a molecular mass of approximately 17 kDa. Researchers found TMEM166 expressed in multiple tissues particularly in brain lung and liver. It is located in the membrane structures within the cell contributing to its mechanistic roles in cellular activities where it interacts with other cellular components to influence important processes like autophagy and apoptosis.
Biological function summary

TMEM166 collaborates with cellular components to promote the regulation of cell death and survival. Its involvement in autophagy a process critical for cellular cleanliness and nutrient recycling highlights its role in ensuring cellular homeostasis. TMEM166 may not function as part of a large protein complex but it works in sync with other autophagy and apoptosis-related factors. Its interactions and functionality can influence cellular response to stress helping to maintain balance in cell populations.

Pathways

TMEM166 plays roles that intersect with major cellular processes. It notably contributes to the apoptosis and autophagy pathways. In the apoptosis pathway TMEM166 can interact with factors like BCL-2 family proteins which regulate cell death to influence whether a cell will undergo programmed death. Within the context of autophagy TMEM166 assists in signaling cascades that regulate the formation and maturation of autophagic vesicles. Its activity can therefore modulate critical responses to nutrient availability and stress.

TMEM166 exhibits associations with neurodegenerative diseases and certain cancers. Aberrations in TMEM166 expression or function may lead to improper regulation of apoptosis fostering environments conducive to cancer development. In neurodegenerative conditions altered TMEM166 activity might affect neuronal survival through its impact on autophagy and apoptosis contributing to disease progression. Studies have hinted at connections between TMEM166 and proteins like LC3B which is key in autophagosome formation offering insights into the target’s potential clinical relevance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.
See full target information EVA1A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36078115

2022

The Degradation of TMEM166 by Autophagy Promotes AMPK Activation to Protect SH-SY5Y Cells Exposed to MPP.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaozhong Liao,Zunshuang Gong,Zhe Wang,Weiyan Yang,Wenjing Liu,Lin Hou,Xiaokun Liu,Junnan Hua,Bin Wang,Ning Li

Orthopaedic surgery 13:616-622 PubMed33620145

2021

H3.3 K36M Mutation as a Clinical Diagnosis Method of Suspected Chondroblastoma Cases.

Applications

Unspecified application

Species

Unspecified reactive species

Haoran Mu,Yafei Jiang,Linghang Xue,Yingqi Hua,Jun Lin,Zhengdong Cai
View all publications
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