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AB325954

Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free

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Rabbit Recombinant Monoclonal TMEM16A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Transfected cell line - Mouse, Human, Mouse samples.

View Alternative Names

Tmem16a, Anoctamin-1, Transmembrane protein 16A, DOG1, ORAOV2, TAOS2, TMEM16A, ANO1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)

This data was developed using ab323181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse salivary gland tissue labeling TMEM16A with ab323181 at 1/1000 (0.532 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse salivary gland.
The section was incubated with ab323181 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)

This data was developed using ab323181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse colon cancer tissue labeling TMEM16A with ab323181 at 1/1000 (0.532 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse colon cancer.
The section was incubated with ab323181 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)

This data was developed using ab323181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling TMEM16A with ab323181 at 1/1000 (0.532 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse colon.
The section was incubated with ab323181 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)

This data was developed using ab323181, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse pancreas tissue staining CFTR with ab325171 at a 1 : 2000 (0.259 ug/ml) dilution, ab323181 anti-TMEM16A used at 1 : 1000 (0.532 ug/ml) dilution and ab181547 anti-Insulin used at a 1 : 20000 (0.053 ug/ml) dilution.

Panel A : anti-CFTR (green; Opal™520), anti-TMEM16A (magenta; Opal™690) and anti-Insulin (gray; Opal™570) on mouse pancreas.
Panel B : anti-CFTR staining centroacinar cells in mouse pancreas.
Panel C : anti-TMEM16A staining epithelium in mouse pancreas.
Panel D : anti-Insulin staining islet cells in mouse pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325171, ab323181 and ab181547 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)

This data was developed using ab323181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling TMEM16A with ab323181 at 1/1000 (0.532 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : Nearly no staining in mouse cardiac muscle.
The section was incubated with ab323181 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)
  • WB

Supplier Data

Western blot - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)

This data was developed using ab323181, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : LNCaP(PMID : 29899325) HEK-293(PMID : 27732935)

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TMEM16A antibody [EPR29617-2] (<a href='/en-us/products/primary-antibodies/tmem16a-antibody-epr29617-2-ab323181'>ab323181</a>) at 1/1000 dilution

Lane 1:

Capan-1 (human pancreatic adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa,36 kDa

false

Exposure time: 10s

Western blot - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)
  • WB

Supplier Data

Western blot - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)

This data was developed using ab323181, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart B16-F0

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1 : 10 seconds lane2-5 : 26 seconds

All lanes:

Western blot - Anti-TMEM16A antibody [EPR29617-2] (<a href='/en-us/products/primary-antibodies/tmem16a-antibody-epr29617-2-ab323181'>ab323181</a>) at 1/1000 dilution

Lane 1:

Mouse breast tissue lysate at 20 kDa

Lane 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

B16-F0 (mouse melanoma epithelial-like cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa,36 kDa

false

Western blot - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)
  • WB

Supplier Data

Western blot - Anti-TMEM16A antibody [EPR29617-2] - BSA and Azide free (AB325954)

This data was developed using ab323181, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse ANO2.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-TMEM16A antibody [EPR29617-2] (<a href='/en-us/products/primary-antibodies/tmem16a-antibody-epr29617-2-ab323181'>ab323181</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse ANO1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse ANO2 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29617-2

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Transfected cell line - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab325954 is the carrier-free version of ab323181

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-activated chloride channel (CaCC) (PubMed : 18724360, PubMed : 22002868, PubMed : 22634729, PubMed : 23840801, PubMed : 24913262, PubMed : 25779870, PubMed : 28561733, PubMed : 28963502, PubMed : 29236684, PubMed : 29236691, PubMed : 31147466, PubMed : 34089532, PubMed : 34433575, PubMed : 35135993). Plays a role in transepithelial anion transport and smooth muscle contraction (PubMed : 28561733, PubMed : 29236684, PubMed : 29236691). Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. Required for CFTR activation by enhancing endoplasmic reticulum Ca(2+) store release and is also required for CFTR membrane expression (By similarity). Required for basal and ATP-dependent mucus secretion in airways and intestine, probably by controlling exocytosis of mucus-filled granules by providing Ca(2+) to an apical signaling compartment (PubMed : 30586313). Contributes to airway mucus expression induced by interleukins IL3 and IL8 and by the asthma-associated protein CLCA1 and is required for expression of mucin MUC5AC (By similarity). However, was shown in another study not to be required for MUC5AC expression (By similarity). Plays a role in the propagation of Ca(2+) waves in Kolliker's organ in the cochlea and contributes to the refinement of auditory brainstem circuitries prior to hearing onset (PubMed : 35129434). In vomeronasal sensory neurons, modulates spontaneous firing patterns in the absence of stimuli as well as the firing pattern of pheromone-evoked activity (PubMed : 34433575). Responsible for calcium-activated chloride channel activity in type I taste cells of the vallate papillae (PubMed : 34089532). Acts as a heat sensor in nociceptive neurons (PubMed : 22634729). In dorsal root ganglion neurons, plays a role in mediating non-histaminergic Mas-related G-protein coupled receptor (MRGPR)-dependent itching, acting as a downstream effector of MRGPRs (PubMed : 35135993). In the developing brain, required for the Ca(2+)-dependent process extension of radial glial cells (PubMed : 31147466).
See full target information Ano1

Additional targets

ANO1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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