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Rabbit Polyclonal TMEM16B antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human ANO2 conjugated to Keyhole Limpet Haemocyanin.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human ANO2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQ90
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Expected
Tested
Mouse
Predicted
Predicted
Rat
Tested
Expected

Tested
Tested

Species

Rat

Dilution info

1.00000-2.00000 µg/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.

Alternative names

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Rabbit Polyclonal TMEM16B antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human ANO2 conjugated to Keyhole Limpet Haemocyanin.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human ANO2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQ90
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

TMEM16B influences processes like odor detection and vision. It acts as a component of a larger channel complex involved in the transduction of sensory signals. In olfactory sensory neurons TMEM16B modulates the signaling pathway by which odors lead to nerve impulses making it important for the sense of smell. In the retina it plays a role in photoreceptor signal regulation supporting proper vision.

Activity summary

TMEM16B also known as ANO2 is a calcium-activated chloride channel with a mass of around 116 kDa. It plays a role in ion transport across the cell membrane. TMEM16B is expressed in various tissues most notably in the sensory neurons and the retina where it contributes to the function of sensory systems. Its activity depends on calcium presence allowing the passage of chloride ions which affects membrane potential and cellular signaling.

Pathways

Protein TMEM16B interacts within the sensory transduction pathways including the visual and olfactory pathways. In the olfactory system it works with proteins like CNGA2 in the signaling cascade from odorant molecules to neuronal response. In the visual pathway TMEM16B collaborates with proteins involved in the phototransduction cascade impacting the conversion of light into electrical signals necessary for vision.

Associated diseases and disorders

TMEM16B's malfunction links to anosmia and certain retinal degenerations. Alterations in its activity can lead to loss of smell as TMEM16B is necessary for signal propagation in olfactory neurons. In the retina its dysfunction may contribute to degenerative diseases linking with proteins like rhodopsin which plays a role in light detection. Understanding TMEM16B's role provides insight into these sensory disorders potentially guiding therapeutic strategies.

Product promise

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16B antibody (ab113443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16B antibody (ab113443)

    ab113443, at 5 µg/ml, staining TMEM16B in formalin fixed, paraffin embedded Human small intestine tissue by Immunohistochemistry.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16B antibody (ab113443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16B antibody (ab113443)

    ab113443, at 5 µg/ml, staining TMEM16B in formalin fixed, paraffin embedded Human spleen tissue by Immunohistochemistry.

  • Western blot - Anti-TMEM16B antibody (ab113443), expandable thumbnail

    Western blot - Anti-TMEM16B antibody (ab113443)

    Lane 1: Western blot - Anti-TMEM16B antibody (AB113443) at 1 µg/mL

    Lane 2: Western blot - Anti-TMEM16B antibody (AB113443) at 2 µg/mL

    All lanes: Rat brain tissue lysate

    Predicted band size: 114 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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