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AB91573

Anti-TMEM16B antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal TMEM16B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ANO2.

View Alternative Names

C12orf3, TMEM16B, ANO2, Anoctamin-2, Transmembrane protein 16B

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TMEM16B antibody (AB91573)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TMEM16B antibody (AB91573)

Immunofluorescence of TMEM16B in Human Brain cells using ab91573 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16B antibody (AB91573)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16B antibody (AB91573)

Immunohistochemical analysis of human brain tissue labelling TMEM16B with ab91573 at 5 ug/mL.

Western blot - Anti-TMEM16B antibody (AB91573)
  • WB

Supplier Data

Western blot - Anti-TMEM16B antibody (AB91573)

All lanes:

Western blot - Anti-TMEM16B antibody (ab91573) at 2 µg/mL

All lanes:

Human small intestine tissue lysate

Predicted band size: 114 kDa

Observed band size: 107 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ANO2. The exact immunogen used to generate this antibody is proprietary information.

Q9NQ90

Specificity

This antibody will not cross-react with TMEM16A.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM16B also known as ANO2 is a calcium-activated chloride channel with a mass of around 116 kDa. It plays a role in ion transport across the cell membrane. TMEM16B is expressed in various tissues most notably in the sensory neurons and the retina where it contributes to the function of sensory systems. Its activity depends on calcium presence allowing the passage of chloride ions which affects membrane potential and cellular signaling.
Biological function summary

TMEM16B influences processes like odor detection and vision. It acts as a component of a larger channel complex involved in the transduction of sensory signals. In olfactory sensory neurons TMEM16B modulates the signaling pathway by which odors lead to nerve impulses making it important for the sense of smell. In the retina it plays a role in photoreceptor signal regulation supporting proper vision.

Pathways

Protein TMEM16B interacts within the sensory transduction pathways including the visual and olfactory pathways. In the olfactory system it works with proteins like CNGA2 in the signaling cascade from odorant molecules to neuronal response. In the visual pathway TMEM16B collaborates with proteins involved in the phototransduction cascade impacting the conversion of light into electrical signals necessary for vision.

TMEM16B's malfunction links to anosmia and certain retinal degenerations. Alterations in its activity can lead to loss of smell as TMEM16B is necessary for signal propagation in olfactory neurons. In the retina its dysfunction may contribute to degenerative diseases linking with proteins like rhodopsin which plays a role in light detection. Understanding TMEM16B's role provides insight into these sensory disorders potentially guiding therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.
See full target information ANO2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 293:995-1006 PubMed29187602

2017

TMEM16A and TMEM16B channel proteins generate Ca-activated Cl current and regulate melatonin secretion in rat pineal glands.

Applications

Unspecified application

Species

Unspecified reactive species

Hisao Yamamura,Kaori Nishimura,Yumiko Hagihara,Yoshiaki Suzuki,Yuji Imaizumi
View all publications
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Product promise

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