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AB309572

Anti-TMEM175 antibody [EPR24415-47] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal TMEM175 antibody. Carrier free. Suitable for WB, sELISA and reacts with Human samples.

View Alternative Names

Endosomal/lysosomal proton channel TMEM175, Potassium channel TMEM175, Transmembrane protein 175, hTMEM175, TMEM175

1 Images
Sandwich ELISA - Anti-TMEM175 antibody [EPR24415-47] - BSA and Azide free (Detector) (AB309572)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-TMEM175 antibody [EPR24415-47] - BSA and Azide free (Detector) (AB309572)

Sandwich ELISA of ab309570 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

  • Carrier free

    Anti-TMEM175 antibody [EPR24415-47] (BSA and Azide free)

  • Unconjugated

    Anti-TMEM175 antibody [EPR24415-47]

  • 660 APC

    APC Anti-TMEM175 antibody [EPR24415-47]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-TMEM175 antibody [EPR24415-47]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-TMEM175 antibody [EPR24415-47]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-TMEM175 antibody [EPR24415-47]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TMEM175 antibody [EPR24415-47]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-TMEM175 antibody [EPR24415-47]

  • 578 PE

    PE Anti-TMEM175 antibody [EPR24415-47]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24415-47

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM175 also known as Transmembrane Protein 175 is a channel protein with dual function as a lysosomal potassium channel. It has an approximate mass of 66 kDa. TMEM175 plays a role in ion homeostasis and is expressed mainly in tissues with high levels of lysosomes including the brain and the immune system. This protein aids in the maintenance of the lysosomal environment important for cellular activities.
Biological function summary

This lysosomal protein contributes to the maintenance of ion stability within lysosomes an important factor in lysosomal degradation and overall cellular clearance processes. TMEM175 is an integral component of the lysosomal membrane and participates in ensuring lysosomal pH stability therefore influencing autophagic activity. TMEM175 does not participate in known protein complexes but it is pivotal for preserving lysosomal integrity.

Pathways

TMEM175 actively engages in the lysosomal degradation pathway impacting cellular waste processing. TMEM175 influences endolysosomal trafficking and autophagy which are essential for metabolite recycling. It works alongside proteins like ATPase and lysosomal enzyme hydrolases by controlling ion concentrations which can influence other lysosomal functions and pathways.

TMEM175 has a significant connection with neurodegenerative conditions such as Parkinson's disease. Mutation or dysfunction in TMEM175 impacts lysosomal function linked to protein aggregation often involving alpha-synuclein which serves as a hallmark for such neurodegenerative diseases. Additionally TMEM175 is implicated in lysosomal storage disorders where impaired lysosomal function results in cellular accumulation of substances due to ineffective degradation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-activated proton channel that catalyzes proton efflux from endosomes and lysosomes to maintain a steady-state pH (PubMed : 35333573, PubMed : 35750034, PubMed : 37390818). Activated at low pH (under pH 4.6) by luminal side protons : selectively mediates lysosomal proton release from lysosomes, eliciting a proton leak that balances V-ATPase activity to maintain pH homeostasis (PubMed : 35750034). Regulation of lumenal pH stability is required for autophagosome-lysosome fusion (PubMed : 26317472, PubMed : 32267231). Also acts as a potassium channel at higher pH, regulating potassium conductance in endosomes and lysosomes (PubMed : 26317472, PubMed : 28723891, PubMed : 32228865, PubMed : 32267231, PubMed : 33505021). Constitutes the pore-forming subunit of the lysoK(GF) complex, a complex activated by extracellular growth factors (PubMed : 33505021). The lysoK(GF) complex is composed of TMEM175 and AKT (AKT1, AKT2 or AKT3), a major target of growth factor receptors : in the complex, TMEM175 channel is opened by conformational changes by AKT, leading to its activation (PubMed : 33505021). The lysoK(GF) complex is required to protect neurons against stress-induced damage (PubMed : 33505021).
See full target information TMEM175
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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