Anti-TMEM192 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody (AB236858)

A549 (human lung carcinoma cell line) cells stained for TMEM192 (green) using ab236858 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM192 antibody (AB236858)

Paraffin-embedded human adrenal gland tissue stained for TMEM192 using ab236858 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM192 antibody (AB236858)

Paraffin-embedded human glioma tissue stained for TMEM192 using ab236858 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• IP

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Immunoprecipitation - Anti-TMEM192 antibody (AB236858)

TMEM192 was immunoprecipitated from 0.5 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with ab236858 at 1/200 dilution.

Lane 1 : Control rabbit IgG IP in Jurkat whole cell lysate.

Lane 2 : ab236858 IP in Jurkat whole cell lysate.

Lane 3 : Jurkat whole cell lysate 10 μg (Input).

For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-TMEM192 antibody (ab236858)

Predicted band size: 31 kDa

false

• WB

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Western blot - Anti-TMEM192 antibody (AB236858)

All lanes:

Western blot - Anti-TMEM192 antibody (ab236858) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 31 kDa

false