Rabbit Polyclonal TMEM192 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TMEM192 aa 150 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TMEM192 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IY95
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Orangutan | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Alternative names
Transmembrane protein 192, TMEM192
Recommended products
Rabbit Polyclonal TMEM192 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TMEM192 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TMEM192 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IY95
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TMEM192 also known as Transmembrane Protein 192 has a molecular weight of approximately 25 kDa. It is a lysosomal membrane protein expressed in various tissues including the spleen and thymus. This protein predominantly localizes to the lysosomal compartment where it plays a role in maintaining lysosomal integrity. Researchers often study TMEM192 for its role in membrane stabilization and its interaction with other lysosomal proteins.
- Biological function summary
TMEM192 participates in the regulation of lysosomal functions. It integrates into the lysosomal membrane and contributes to the stability and function of this organelle. While not commonly part of larger protein complexes it influences lysosomal enzymatic activities by stabilizing the lysosomal environment. Its role in regulating pH and transport processes within the lysosome directly affects how the cell processes cellular debris and macromolecules.
- Pathways
TMEM192 interacts with cellular processes related to lysosomal biogenesis and autophagy. It functions closely with lysosomal-associated membrane protein 1 (LAMP1) within the lysosomal pathway which plays a critical role in the degradation and recycling of cellular components. The lysosomal pathway where TMEM192 operates is significant for maintaining cellular homeostasis by regulating autophagic activity and responding to nutrient availability.
- Associated diseases and disorders
Disruptions in TMEM192 function associate with lysosomal storage disorders. Altered expression or mutations in TMEM192 link to Niemann-Pick disease type C which involves the accumulation of lipids within lysosomes. LAMP1 a protein associated with TMEM192 also plays a part in this disease pathway contributing to understanding the mechanism of lysosomal dysregulation in Niemann-Pick and similar disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-TMEM192 antibody (ab236858)
All lanes: Western blot - Anti-TMEM192 antibody (ab236858) at 1/1000 dilution
All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM192 antibody (ab236858)
Paraffin-embedded human adrenal gland tissue stained for TMEM192 using ab236858 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM192 antibody (ab236858)
Paraffin-embedded human glioma tissue stained for TMEM192 using ab236858 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Immunoprecipitation - Anti-TMEM192 antibody (ab236858)
TMEM192 was immunoprecipitated from 0.5 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with ab236858 at 1/200 dilution.
Lane 1: Control rabbit IgG IP in Jurkat whole cell lysate.
Lane 2: ab236858 IP in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate 10 μg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
All lanes: Immunoprecipitation - Anti-TMEM192 antibody (ab236858)
Predicted band size: 31 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody (ab236858)
A549 (human lung carcinoma cell line) cells stained for TMEM192 (green) using ab236858 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C
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