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AB186737

Anti-TMEM192 antibody [EPR14330-67]

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(4 Publications)

Rabbit Recombinant Monoclonal TMEM192 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Transmembrane protein 192, TMEM192

2 Images
Flow Cytometry (Intracellular) - Anti-TMEM192 antibody [EPR14330-67] (AB186737)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TMEM192 antibody [EPR14330-67] (AB186737)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedU-87 MG cells labeling TMEM192 with ab186737 at 1/180 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed bygoat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot - Anti-TMEM192 antibody [EPR14330-67] (AB186737)
  • WB

Supplier Data

Western blot - Anti-TMEM192 antibody [EPR14330-67] (AB186737)

All lanes:

Western blot - Anti-TMEM192 antibody [EPR14330-67] (ab186737) at 1/10000 dilution

Lane 1:

U87-MG cell lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Lane 3:

Human fetal lung lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

false

  • Carrier free

    Anti-TMEM192 antibody [EPR14330-67] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14330-67

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/180", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM192 also known as Transmembrane Protein 192 has a molecular weight of approximately 25 kDa. It is a lysosomal membrane protein expressed in various tissues including the spleen and thymus. This protein predominantly localizes to the lysosomal compartment where it plays a role in maintaining lysosomal integrity. Researchers often study TMEM192 for its role in membrane stabilization and its interaction with other lysosomal proteins.
Biological function summary

TMEM192 participates in the regulation of lysosomal functions. It integrates into the lysosomal membrane and contributes to the stability and function of this organelle. While not commonly part of larger protein complexes it influences lysosomal enzymatic activities by stabilizing the lysosomal environment. Its role in regulating pH and transport processes within the lysosome directly affects how the cell processes cellular debris and macromolecules.

Pathways

TMEM192 interacts with cellular processes related to lysosomal biogenesis and autophagy. It functions closely with lysosomal-associated membrane protein 1 (LAMP1) within the lysosomal pathway which plays a critical role in the degradation and recycling of cellular components. The lysosomal pathway where TMEM192 operates is significant for maintaining cellular homeostasis by regulating autophagic activity and responding to nutrient availability.

Disruptions in TMEM192 function associate with lysosomal storage disorders. Altered expression or mutations in TMEM192 link to Niemann-Pick disease type C which involves the accumulation of lipids within lysosomes. LAMP1 a protein associated with TMEM192 also plays a part in this disease pathway contributing to understanding the mechanism of lysosomal dysregulation in Niemann-Pick and similar disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TMEM192
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 32: PubMed40999974

2025

Atractylodin inhibits ferroptosis in sepsis‑induced acute gastrointestinal injury via SIRT3/PRDX3.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Xia Hu,Ming-Qi Chen,Jian-Lin Wang,Tao Wu,Hai-Dong Zhang,Tong-Tong Li,Hui Gao,Yu Bai

The Journal of clinical investigation 135: PubMed39724071

2024

Tagless LysoIP for immunoaffinity enrichment of native lysosomes from clinical samples.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Saarela,Pawel Lis,Sara Gomes,Raja S Nirujogi,Wentao Dong,Eshaan Rawat,Sophie Glendinning,Karolina Zeneviciute,Enrico Bagnoli,Rotimi Fasimoye,Cindy Lin,Kwamina Nyame,Fanni A Boros,Friederike Zunke,Frederic Lamoliatte,Sadik Elshani,Matthew Jaconelli,Judith Jm Jans,Margriet A Huisman,Christian Posern,Lena M Westermann,Angela Schulz,Peter M van Hasselt,Dario R Alessi,Monther Abu-Remaileh,Esther M Sammler

Nature communications 15:1516 PubMed38374070

2024

A SPLICS reporter reveals [Formula: see text]-synuclein regulation of lysosome-mitochondria contacts which affects TFEB nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Flavia Giamogante,Lucia Barazzuol,Francesca Maiorca,Elena Poggio,Alessandra Esposito,Anna Masato,Gennaro Napolitano,Alessio Vagnoni,Tito Calì,Marisa Brini

Cellular and molecular life sciences : CMLS 78:2341-2353 PubMed32986127

2020

Antioxidant functions of DHHC3 suppress anti-cancer drug activities.

Applications

Unspecified application

Species

Unspecified reactive species

Chandan Sharma,Wei Yang,Hanno Steen,Michael R Freeman,Martin E Hemler
View all publications
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Product promise

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