AB250834

Anti-TMEM192 antibody [EPR14330] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TMEM192 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Transmembrane protein 192, TMEM192

4 Images

Flow Cytometry (Intracellular) - Anti-TMEM192 antibody [EPR14330] - BSA and Azide free (AB250834)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TMEM192 antibody [EPR14330] - BSA and Azide free (AB250834)

This data was developed using ab185545, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis using ab185545 at 1/240 dilution on U87-MG cells. Secondary ab : Goat anti rabbit IgG (FITC) at 1/250 dilution. Fixative : 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG.

Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody [EPR14330] - BSA and Azide free (AB250834)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody [EPR14330] - BSA and Azide free (AB250834)

This data was developed using ab185545, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HepG2 cells labeling TMEM192 with ab185545 at 1/100 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative : 4% paraformaldehyde. Counter stain : Dapi.

Supplier Data

Western blot - Anti-TMEM192 antibody [EPR14330] - BSA and Azide free (AB250834)

This data was developed using ab185545, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TMEM192 antibody [EPR14330] (<a href='/en-us/products/primary-antibodies/tmem192-antibody-epr14330-ab185545'>ab185545</a>) at 1/2000 dilution

All lanes:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-TMEM192 antibody [EPR14330] - BSA and Azide free (AB250834)

This data was developed using ab185545, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TMEM192 antibody [EPR14330] (<a href='/en-us/products/primary-antibodies/tmem192-antibody-epr14330-ab185545'>ab185545</a>) at 1/10000 dilution

All lanes:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14330

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250834 is the carrier-free version of ab185545.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM192 also known as Transmembrane Protein 192 has a molecular weight of approximately 25 kDa. It is a lysosomal membrane protein expressed in various tissues including the spleen and thymus. This protein predominantly localizes to the lysosomal compartment where it plays a role in maintaining lysosomal integrity. Researchers often study TMEM192 for its role in membrane stabilization and its interaction with other lysosomal proteins.

Biological function summary

TMEM192 participates in the regulation of lysosomal functions. It integrates into the lysosomal membrane and contributes to the stability and function of this organelle. While not commonly part of larger protein complexes it influences lysosomal enzymatic activities by stabilizing the lysosomal environment. Its role in regulating pH and transport processes within the lysosome directly affects how the cell processes cellular debris and macromolecules.

Pathways

TMEM192 interacts with cellular processes related to lysosomal biogenesis and autophagy. It functions closely with lysosomal-associated membrane protein 1 (LAMP1) within the lysosomal pathway which plays a critical role in the degradation and recycling of cellular components. The lysosomal pathway where TMEM192 operates is significant for maintaining cellular homeostasis by regulating autophagic activity and responding to nutrient availability.

Disruptions in TMEM192 function associate with lysosomal storage disorders. Altered expression or mutations in TMEM192 link to Niemann-Pick disease type C which involves the accumulation of lipids within lysosomes. LAMP1 a protein associated with TMEM192 also plays a part in this disease pathway contributing to understanding the mechanism of lysosomal dysregulation in Niemann-Pick and similar disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information TMEM192

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TMEM192 antibody [EPR14330] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-TMEM192 antibody [EPR14330] - BSA and Azide free (AB250834)

Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody [EPR14330] - BSA and Azide free (AB250834)

Western blot - Anti-TMEM192 antibody [EPR14330] - BSA and Azide free (AB250834)

Western blot - Anti-TMEM192 antibody [EPR14330] - BSA and Azide free (AB250834)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise