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AB238740

Anti-TMEM2 antibody

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(1 Publication)

Rabbit Polyclonal TMEM2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CEMIP2 aa 700-900.

View Alternative Names

KIAA1412, TMEM2, CEMIP2, Cell surface hyaluronidase CEMIP2, Cell migration-inducing hyaluronidase 2, Transmembrane protein 2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB238740)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB238740)

Paraffin-embedded human brain tissue stained for TMEM2 using ab238740 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-TMEM2 antibody (AB238740)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMEM2 antibody (AB238740)

A549 (Human lung carcinoma cell line) cells stained for TMEM2 (Green) using ab238740 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counterstain is DAPI.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CEMIP2 aa 700-900. The exact immunogen used to generate this antibody is proprietary information.

Q9UHN6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM2 also known as Transmembrane Protein 2 is a protein involved in the hydrolysis of hyaluronan a component of the extracellular matrix. TMEM2 is a single-pass type II transmembrane protein with a molecular mass of approximately 150 kDa. It is expressed in various tissues with heightened expression in the heart skeletal muscle and connective tissues. Researchers have noted its importance in the remodeling of the matrix during developmental processes and tissue repair.
Biological function summary

The protein TMEM2 plays a role in hyaluronan turnover which affects processes like cell motility and proliferation. It is not part of a larger protein complex but its activity influences the availability of hyaluronan fragments that interact with other cell surface receptors. This interaction can influence cellular responses such as inflammation and tissue modeling.

Pathways

TMEM2 is significant in the matrix degradation pathway impacting cellular and tissue development signaling. It is related to the extracellular matrix organization pathway where its function in hyaluronan degradation directly affects other matrix proteins. This interaction is often seen with proteins such as CD44 and RHAMM which are known to bind hyaluronan fragments and mediate further signaling.

TMEM2 mutations or dysfunctions have links to cardiovascular diseases and certain cancers. Its role in hyaluronan turnover has implications in pathological processes like tumor metastasis given that altered extracellular matrix can affect cancer cell mobility. The protein interacts with CD44 a receptor involved in tumor progression and metastasis highlighting its potential importance in oncological research. Understanding TMEM2 and its pathways could provide insights into therapeutic targets for treating cancer and cardiovascular conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 10-5 kDa fragments (PubMed : 37527776). Very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate. Has an essential function in systemic hyaluronan catabolism and turnover and regulates cell adhesion and migration via hyaluronan degradation at focal adhesion sites (By similarity). Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity).
See full target information CEMIP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:5323-5333 PubMed34499008

2021

The tumor-suppressive effects of alpha-ketoglutarate-dependent dioxygenase FTO via N6-methyladenosine RNA methylation on bladder cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfa Yi,Yong Yu,Yafei Li,Juan Yang,Shuying Gao,Lingfen Xu
View all publications
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