Rabbit Polyclonal TMEM205 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TMEM205 aa 100 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TMEM205 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6UW68
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
In cancer cells, plays a role in resistance to the chemotherapeutic agent cisplatin.
Alternative names
UNQ501/PRO1018, TMEM205, Transmembrane protein 205
Recommended products
Rabbit Polyclonal TMEM205 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TMEM205 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TMEM205 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6UW68
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TMEM205 also known as Transmembrane Protein 205 is an integral membrane protein involved in transportation processes across cellular membranes. It weighs approximately 26 kDa and primarily resides in the plasma membranes of cells. TMEM205 is expressed in a broad range of tissues with notable expression in the liver kidney lung and heart. This protein contributes to cellular processes by participating in the movement of various molecules across the cell envelope.
- Biological function summary
It plays a role in cellular export mechanisms and likely intersects with the multidrug resistance (MDR) pathways. It does not form part of any larger protein complex but functions independently to modulate cellular response to different substances. Its presence associates with cellular export systems and impacts the ability of the cell to handle xenobiotics which are foreign chemical substances found within an organism.
- Pathways
TMEM205 functions in the xenobiotic metabolism regulatory pathways and cellular stress response pathways. It interacts with proteins such as MDR1 (P-glycoprotein) and ABC transporters which are significant in drug resistance mechanisms. These interactions suggest its involvement in detoxification processes allowing cells to expel potentially harmful compounds and adapt to environmental changes.
- Associated diseases and disorders
TMEM205 has been connected to chemotherapy resistance in cancer notably influencing treatment outcomes. Its association with proteins like MDR1 contributes to this resistance by expelling chemotherapeutic agents from cells making treatment less effective. Additionally research indicates it may play a role in certain liver dysfunctions linking it to overall hepatic health and challenging the management of related disorders. Understanding TMEM205's function can aid in developing strategies to counteract drug resistance and support liver health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM205 antibody (ab224516)
Paraffin-embedded human urinary bladder tissue stained for TMEM205 using ab224516 at 1/1000 dilution in immunohistochemical analysis.
Western blot - Anti-TMEM205 antibody (ab224516)
All lanes: Western blot - Anti-TMEM205 antibody (ab224516) at 1/100 dilution
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3: Human plasma (IgG/HSA depleted)
Lane 4: Human liver lysate
Lane 5: Human tonsil lysate
Developed using the ECL technique.
Predicted band size: 21 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TMEM205 antibody (ab224516)
PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for TMEM205 (green) using ab224516 at 4 μg/ml in ICC/IF.
Downloads
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